Cucumber(Cucumis sativus L.)is an agriculturally and economically important vegetable crop worldwide.The thickness of mesocarp is an important quality pertaining to edibility.The development of mesocarp,however,has re...Cucumber(Cucumis sativus L.)is an agriculturally and economically important vegetable crop worldwide.The thickness of mesocarp is an important quality pertaining to edibility.The development of mesocarp,however,has received little attention.In this study,we selected one thick mesocarp line D8 to analyze the gene expression profiles of mesocarp at 0,3,6,and 9 days after pollination(DAP)by RNA sequencing.Genes mainly related to ribosomes,carbon metabolism,biosynthesis of amino acids,and oxidative phosphorylation were up-regulated at 3 DAP.From 3 to 6 DAP,the genes related to oxidative phosphorylation,photosynthesis,and phagosome were highly induced.From6 to 9 DAP,the genes related to plant hormone signal transduction were highly induced.Differentially expressed genes linked to cyclin,hormone biosynthesis and signaling,carbon metabolism,and transcription factors MYB and WRKY were common up-regulated at 3,6,9 DAP in comparison with 0 DAP.The auxin-responsive and cytokinin-responsive genes were significantly up-regulated in mesocarp development.The higher expression levels of the auxin-responsive gene indole-3-acetic acid-amido synthetase 3.6 and the cytokinin response regulator 3/4b in the thick mesocarp cultivar D8 than in the thin mesocarp cultivar XUE1 might have explained the different growth rates of the mesocarp.展开更多
近年来社会拥挤逐渐成为营销领域的一个研究热点,但仍较为零散,大量研究着重于探寻引起社会拥挤的原因等。消费行为往往受到微妙的环境因素的影响,随着拥挤状况在消费场所中日益频现,人们的消费倾向会发生改变,尤其在不同情境下,是否会...近年来社会拥挤逐渐成为营销领域的一个研究热点,但仍较为零散,大量研究着重于探寻引起社会拥挤的原因等。消费行为往往受到微妙的环境因素的影响,随着拥挤状况在消费场所中日益频现,人们的消费倾向会发生改变,尤其在不同情境下,是否会对消费群体的特殊消费行为产生影响尚未进行研究。鉴于此,本文针对社会拥挤,借助pajek针对web of science上核心论文进行网络分析,在现有研究成果的可视化基础,构建以食品与医疗行业为整体的研究框架,进行社会拥挤对放纵性消费的双情境实验对比研究。结果表明,餐饮情境下社会拥挤会显著降低放纵性消费水平,而在医疗情境下社会拥挤则会显著增加放纵性消费的水平,以期为学术研究和企业的营销管理提供参考和建议。展开更多
Based on the absorption property of a diazine that can be formed by reaction of glyoxal and 3-methyl-2-benzothiazolinone hydrazone(MBTH)in the Ultravioletvisible(UV-vis)spectral region,a HPLC method was developed for ...Based on the absorption property of a diazine that can be formed by reaction of glyoxal and 3-methyl-2-benzothiazolinone hydrazone(MBTH)in the Ultravioletvisible(UV-vis)spectral region,a HPLC method was developed for the determination of glyoxal in acetaldehyde solution.Glyoxal was derivatised from MBTH and the derivatives(diazine)were analyzed by HPLC for identification and quantification.The determination was performed on a ZORBAX Eclipse XDB-C18 column(4.6250 mm,5 mm)at 35℃ with an injection volume of 10 mL,using a mixture of acetonitrile-water solvent(99∶5,v∶v)as a mobile phase with a flow rate of 0.8 mL·min^(–1).The proper derivative reaction conditions were the temperature of 70℃,MBTH to carbonyl molar ratio of 12,and reaction time of 110 min.The glyoxal diazine was a yellow dye with a maximum molar absorptivity at 401 nm and its retention time was 5.2 min under optimal HPLC conditions.The standard curve for glyoxal had a strong linear relationship with a regression coefficient(R2=0.999)in the range of 0.002–0.020 g·L^(–1).The analysis of glyoxal in an oxidising solution gave accurate results with a relative standard deviation(RSD)value of 0.55%.The average relative recovery was 102%.This efficient HPLC technique is also proposed for detecting other dicarbonyl compounds besides glyoxal.展开更多
基金the National Key Research and Development Program of China(Grant No.2018YFD0100702)Major agricultural varieties creation project in Jiangsu Province(Grant No.PZCZ201720)+1 种基金Jiangsu Agricultural Science and Technology Innovation Fund(Grant No.CX(19)3027)the Modern Agriculture Foundation of Yangzhou(Grant No.YZ2019042).
文摘Cucumber(Cucumis sativus L.)is an agriculturally and economically important vegetable crop worldwide.The thickness of mesocarp is an important quality pertaining to edibility.The development of mesocarp,however,has received little attention.In this study,we selected one thick mesocarp line D8 to analyze the gene expression profiles of mesocarp at 0,3,6,and 9 days after pollination(DAP)by RNA sequencing.Genes mainly related to ribosomes,carbon metabolism,biosynthesis of amino acids,and oxidative phosphorylation were up-regulated at 3 DAP.From 3 to 6 DAP,the genes related to oxidative phosphorylation,photosynthesis,and phagosome were highly induced.From6 to 9 DAP,the genes related to plant hormone signal transduction were highly induced.Differentially expressed genes linked to cyclin,hormone biosynthesis and signaling,carbon metabolism,and transcription factors MYB and WRKY were common up-regulated at 3,6,9 DAP in comparison with 0 DAP.The auxin-responsive and cytokinin-responsive genes were significantly up-regulated in mesocarp development.The higher expression levels of the auxin-responsive gene indole-3-acetic acid-amido synthetase 3.6 and the cytokinin response regulator 3/4b in the thick mesocarp cultivar D8 than in the thin mesocarp cultivar XUE1 might have explained the different growth rates of the mesocarp.
文摘近年来社会拥挤逐渐成为营销领域的一个研究热点,但仍较为零散,大量研究着重于探寻引起社会拥挤的原因等。消费行为往往受到微妙的环境因素的影响,随着拥挤状况在消费场所中日益频现,人们的消费倾向会发生改变,尤其在不同情境下,是否会对消费群体的特殊消费行为产生影响尚未进行研究。鉴于此,本文针对社会拥挤,借助pajek针对web of science上核心论文进行网络分析,在现有研究成果的可视化基础,构建以食品与医疗行业为整体的研究框架,进行社会拥挤对放纵性消费的双情境实验对比研究。结果表明,餐饮情境下社会拥挤会显著降低放纵性消费水平,而在医疗情境下社会拥挤则会显著增加放纵性消费的水平,以期为学术研究和企业的营销管理提供参考和建议。
基金Financial support by the China Petroleum and Chemical Corporation is gratefully acknowledged.
文摘Based on the absorption property of a diazine that can be formed by reaction of glyoxal and 3-methyl-2-benzothiazolinone hydrazone(MBTH)in the Ultravioletvisible(UV-vis)spectral region,a HPLC method was developed for the determination of glyoxal in acetaldehyde solution.Glyoxal was derivatised from MBTH and the derivatives(diazine)were analyzed by HPLC for identification and quantification.The determination was performed on a ZORBAX Eclipse XDB-C18 column(4.6250 mm,5 mm)at 35℃ with an injection volume of 10 mL,using a mixture of acetonitrile-water solvent(99∶5,v∶v)as a mobile phase with a flow rate of 0.8 mL·min^(–1).The proper derivative reaction conditions were the temperature of 70℃,MBTH to carbonyl molar ratio of 12,and reaction time of 110 min.The glyoxal diazine was a yellow dye with a maximum molar absorptivity at 401 nm and its retention time was 5.2 min under optimal HPLC conditions.The standard curve for glyoxal had a strong linear relationship with a regression coefficient(R2=0.999)in the range of 0.002–0.020 g·L^(–1).The analysis of glyoxal in an oxidising solution gave accurate results with a relative standard deviation(RSD)value of 0.55%.The average relative recovery was 102%.This efficient HPLC technique is also proposed for detecting other dicarbonyl compounds besides glyoxal.
