In this paper, we conduct research on the precision Japanese literary translation mode from the general perspectives of cultural equivalence and metaphor. Language is a nation all enrichment culture information, the s...In this paper, we conduct research on the precision Japanese literary translation mode from the general perspectives of cultural equivalence and metaphor. Language is a nation all enrichment culture information, the semantics of the accurate grasp, flexible use of expression, this requires the translator to the nation＇ s culture will have a more profound understanding. Accumulating knowledge of Japanese culture, to understand the background knowledge of the translator of the original has a very important role in the accuracy of the translation in the context of Japanese culture, not in a few words to give full expression to the Japanese national characteristics of the life and customs. Under this background, our research proposes the novel paradigm for the corresponding researches that will enhance our understanding of the Japanese which is meaningful.展开更多
The application of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)can be limited due to a lack of compatible protospacer adjacent motif(PAM)sequences in the DNA reg...The application of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)can be limited due to a lack of compatible protospacer adjacent motif(PAM)sequences in the DNA regions of interest.Recently,SpRY,a variant of Streptococcus pyogenes Cas9(SpCas9),was reported,which nearly completely fulfils the PAM requirement.Meanwhile,PAMs for Sp RY have not been well addressed.In our previous study,we developed the PAM Definition by Observable Sequence Excision(PAM-DOSE)and green fluorescent protein(GFP)-reporter systems to study PAMs in human cells.Herein,we endeavored to identify the PAMs of SpRY with these two methods.The results indicated that 5’-NRN-3’,5’-NTA-3’,and 5’-NCK-3’could be considered as canonical PAMs.5’-NCA-3’and 5’-NTK-3’may serve as non-priority PAMs.At the same time,PAM of 5’-NYC-3’is not recommended for human cells.These findings provide further insights into the application of SpRY for human genome editing.展开更多
文摘In this paper, we conduct research on the precision Japanese literary translation mode from the general perspectives of cultural equivalence and metaphor. Language is a nation all enrichment culture information, the semantics of the accurate grasp, flexible use of expression, this requires the translator to the nation＇ s culture will have a more profound understanding. Accumulating knowledge of Japanese culture, to understand the background knowledge of the translator of the original has a very important role in the accuracy of the translation in the context of Japanese culture, not in a few words to give full expression to the Japanese national characteristics of the life and customs. Under this background, our research proposes the novel paradigm for the corresponding researches that will enhance our understanding of the Japanese which is meaningful.
基金supported by Lin HE’s Academician Workstation of New Medicine and Clinical Translation(No.18331105)the Program for Basic Science and Technology Cooperation Projects of Wenzhou City(No.H22010011),China。
文摘The application of clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated proteins(Cas)can be limited due to a lack of compatible protospacer adjacent motif(PAM)sequences in the DNA regions of interest.Recently,SpRY,a variant of Streptococcus pyogenes Cas9(SpCas9),was reported,which nearly completely fulfils the PAM requirement.Meanwhile,PAMs for Sp RY have not been well addressed.In our previous study,we developed the PAM Definition by Observable Sequence Excision(PAM-DOSE)and green fluorescent protein(GFP)-reporter systems to study PAMs in human cells.Herein,we endeavored to identify the PAMs of SpRY with these two methods.The results indicated that 5’-NRN-3’,5’-NTA-3’,and 5’-NCK-3’could be considered as canonical PAMs.5’-NCA-3’and 5’-NTK-3’may serve as non-priority PAMs.At the same time,PAM of 5’-NYC-3’is not recommended for human cells.These findings provide further insights into the application of SpRY for human genome editing.
