Deep neural network based on multi-level wavelet and attention for structured illumination microscopy

Structured illumination microscopy(SIM)is a popular and powerful super-resolution(SR)technique in biomedical research.However,the conventional reconstruction algorithm for SIM heavily relies on the accurate prior knowledge of illumination patterns and signal-to-noise ratio(SNR)of raw images.To obtain high-quality SR images,several raw images need to be captured under high fluorescence level,which further restricts SIM’s temporal resolution and its applications.Deep learning(DL)is a data-driven technology that has been used to expand the limits of optical microscopy.In this study,we propose a deep neural network based on multi-level wavelet and attention mechanism(MWAM)for SIM.Our results show that the MWAM network can extract high-frequency information contained in SIM raw images and accurately integrate it into the output image,resulting in superior SR images compared to those generated using wide-field images as input data.We also demonstrate that the number of SIM raw images can be reduced to three,with one image in each illumination orientation,to achieve the optimal tradeoff between temporal and spatial resolution.Furthermore,our MWAM network exhibits superior reconstruction ability on low-SNR images compared to conventional SIM algorithms.We have also analyzed the adaptability of this network on other biological samples and successfully applied the pretrained model to other SIM systems.

Implications of the Stellar Mass Density of High-z Massive Galaxies from JWST on Warm Dark Matter

A significant excess of the stellar mass density at high redshift has been discovered from the early data release of James Webb Space Telescope(JWST),and it may require a high star formation efficiency.However,this will lead to large number density of ionizing photons in the epoch of reionization(EoR),so that the reionization history will be changed,which can arise tension with the current EoR observations.Warm dark matter(WDM),via the free streaming effect,can suppress the formation of small-scale structure as well as low-mass galaxies.This provides an effective way to decrease the ionizing photons when considering a large star formation efficiency in high-z massive galaxies without altering the cosmic reionization history.On the other hand,the constraints on the properties of WDM can be derived from the JWST observations.In this work,we study WDM as a possible solution to reconcile the JWST stellar mass density of high-z massive galaxies and reionization history.We find that,the JWST high-z comoving cumulative stellar mass density alone has no significant preference for either CDM or WDM model.But using the observational data of other stellar mass density measurements and reionization history,we obtain that the WDM particle mass with mw=0.51_(-0.12)^(+0.22) keV and star formation efficiency parameter f_(*)^(0)> 0.39 in 2σ confidence level can match both the JWST high-z comoving cumulative stellar mass density and the reionization history.

Nintedanib induces apoptosis in human pterygium cells through the FGFR2-ERK signalling pathway

AIM:To investigate whether nintedanib can inhibit pterygium cells through the fibroblast growth factor receptor 2(FGFR2)/extracellular-signal-regulated kinase(ERK)pathway.METHODS:Human primary pterygium cells were cultured in vitro.After treatment with nintedanib,the cell morphology was observed under microscopy,the morphological changes of the nucleus were observed after DAPI staining,apoptosis was analyzed by Annexin-V FITC/PI double staining,and the changes of apoptosis-associated proteins were detected by Western blot.The binding ability of nintedanib to FGFR2 was predicted by molecular docking.Finally,by silencing FGFR2,we explored whether nintedanib inhibited FGFR2/ERK pathway.RESULTS:The results showed that nintedanib inhibited the growth of pterygium cells and caused nuclear pyknosis.The results of Annexin-VFITC/PI double staining showed that nintedanib was able to induce early and late apoptosis of pterygium cells,significantly increasing the expression of apoptosis-associated proteins Bax and cleaved-Caspase3(P<0.05),and reducing the expression of Bcl-2(P<0.05).In addition,nintedanib significantly inhibited ERK1/2 phosphorylation through FGFR2(P<0.05).After silencing the expression of FGFR2,there was no significant difference in the inhibition of ERK1/2 phosphorylation by nintedanib(P>0.05).CONCLUSION:Nintedanib induces apoptosis of pterygium cells by inhibiting FGFR2/ERK pathway.

A sutureless technique for securing leaking sclerotomies with viscoelastic substances in 23-gauge microincision vitrectomy surgery

AIM:To introduce and evaluate the clinical efficacy of a new technique,the use of viscoelastic substances(VS)to close leaking sclerotomy in 23G microincision vitrectomy,and to observe its effect on the visual acuity and intraocular pressure(IOP)of patients.METHODS:Patients who underwent 23G vitrectomy in Ningbo Eye Hospital before the use of VS technique(June 2019 to September 2020)and after the use of VS technique(October 2020 to December 2021)were selected as the subjects of this study.The above cases underwent operation by the same surgeon and were retrospectively analyzed.VS technique was used as the alternative to suturing,in which a small amount of VS was injected at the leaking sclerotomy and then gently massaged to confirm leaking sclerotomy closure.RESULTS:A total of 174 eyes were covered in the study,including 84 eyes in the control group(before the use of VS technique)and 90 eyes in the VS technique group.The number of eyes that needed to be sutured decreased considerably from 42.9%in the control group to 3.3%in the VS technique group,and the proportion of subconjunctival hemorrhage at 1-2d after surgery decreased remarkably from 35.7%in the control group to 2.2%in the VS technique group.No substantial differences in the incidence of mean IOP and low IOP were found between 1-2 and 3-20d after surgery in the VS technique group.No major complications associated with VS technique were identified during the study.CONCLUSION:In 23G microincision vitrectomy,VS technique is a safe,simple,and effective method to close leaking sclerotomy.

Characteristics of sub-synchronous oscillation in grid-connected wind farm system

With the rapid development of wind power, wind turbines are accompanied by a large quantity of power electronic converters connected to the grid, causing changes in the characteristics of the power system and leading to increasingly serious sub-synchronous oscillation (SSO) problems, which urgently require the generalized classification and characterization of the emerging oscillation problems. This paper classifies and characterizes the emerging types of SSO caused by grid-connected wind turbines to address these issues. Finally, the impact of the typical system parameters changes on the oscillation pattern is analyzed in depth to provide effective support for the subsequent suppression and prevention of SSO.

Cross-correlation Forecast of CSST Spectroscopic Galaxy and MeerKAT Neutral Hydrogen Intensity Mapping Surveys

Cross-correlating the data on neutral hydrogen(HⅠ)21 cm intensity mapping with galaxy surveys is an effective method to extract astrophysical and cosmological information.In this work,we investigate the cross-correlation of MeerKAT single-dish mode HⅠintensity mapping and China Space Station Telescope(CSST)spectroscopic galaxy surveys.We simulate a survey area of~300 deg~2 of MeerKAT and CSST surveys at z=0.5 using MultiDark N-body simulation.The PC A algorithm is applied to remove the foregrounds of HⅠintensity mapping,and signal compensation is considered to solve the signal loss problem in HⅠ-galaxy cross power spectrum caused by the foreground removal process.We find that from CSST galaxy auto and MeerKAT-CSST cross power spectra,the constraint accuracy of the parameter productΩ_(HⅠ)b_(HⅠ)r_(HⅠ,g)can reach~1%,which is about one order of magnitude higher than the current results.After performing the full MeerKAT HⅠintensity mapping survey with5000 deg~2 survey area,the accuracy can be enhanced to<0.3%.This implies that the MeerKAT-CSST cross-correlation can be a powerful tool to probe the cosmic HⅠproperty and the evolution of galaxies and the Universe.

转录组中LncRNA在猪上的研究进展

长链非编码RNA(lncRNA)曾经被人们认为是转录过程中的“垃圾”,翻译过程中的“噪声”。但越来越多的研究发现其在转录的整个过程中具有调控作用,可以参与细胞内的多种生物学调控。之前,lncRNA在人类的疾病、细胞分化等方面得到了高度注视和研究,而在家畜上的研究相对滞后,文章主要综述了lncRNA的生物学功能及在猪上的研究进展,为lncRNA在家畜上研究和应用提供参考。

聚(3,4-二溴噻吩)复合光子晶体的制备及电致变色性能

研究了二氧化硅复合聚(3,4-二溴噻吩)光子晶体(SiO2@PDBrTh)的制备及电致变色性能。首先采用改进St?ber法合成SiO2微球,再通过三电极体系制备SiO2@PDBrTh复合光子晶体,在相同条件下,与以ITO玻璃为工作电极制备得到的纯PDBrTh薄膜做对比。结果表明,将光子晶体的有序结构引入到PDBrTh中,对比度提高了15.4%,着色和褪色响应时间也均有所加快,复合薄膜的颜色从黄绿色变化到浅蓝色,其色差大于纯PDBrTh薄膜。这表明,将光子晶体的有序结构引入PDBrTh中,可以提高其电致变色性能,便于更好地应用于智能窗、传感器等领域。

Experimental Realization of an Intrinsic Magnetic Topological Insulator

An intrinsic magnetic topological insulator(TI) is a stoichiometric magnetic compound possessing both inherent magnetic order and topological electronic states. Such a material can provide a shortcut to various novel topological quantum effects but remained elusive experimentally for a long time. Here we report the experimental realization of thin films of an intrinsic magnetic TI, MnBi2Te4, by alternate growth of a Bi2Te3 quintuple layer and a MnTe bilayer with molecular beam epitaxy. The material shows the archetypical Dirac surface states in angle-resolved photoemission spectroscopy and is demonstrated to be an antiferromagnetic topological insulator with ferromagnetic surfaces by magnetic and transport measurements as well as first-principles calculations. The unique magnetic and topological electronic structures and their interplays enable the material to embody rich quantum phases such as quantum anomalous Hall insulators and axion insulators at higher temperature and in a well-controlled way.

ABO blood type,diabetes and risk of gastrointestinal cancer in northern China

AIM:To explore the potential risk factors related to gastrointestinal cancer in northern China.METHODS:A total of 3314 cases of gastrointestinal cancer(esophageal,gastric,pancreatic and biliary) and 2223 controls(including healthy individuals,glioma and thyroid cancer) were analyzed by case-control study.Multivariable logistic regression analysis was applied to evaluate the association between different cancers and hepatitis B surface antigen,sex,age,blood type,diabetes,or family history of cancer.RESULTS:Type 2 diabetes was significantly associated with gastric,biliary and pancreatic cancer with an OR of 2.0-3.0.Blood type B was significantly associated with esophageal cancer [odd ratio(OR) = 1.53,95% confidence interval(CI) = 1.10-2.14] and biliary cancer(OR = 1.49,95% CI = 1.09-2.05).The prevalence of type 2 diabetes was significantly higher in gastric,biliary and pancreatic cancers compared with other groups,with ORs ranging between 2.0 and 3.0.Family history of cancer was strongly associated with gastrointestinal compared with other cancers.CONCLUSION:Blood type B individuals are susceptible to esophageal and biliary cancer.Type 2 diabetes is significantly associated with gastric,biliary and especially pancreatic cancer.

Silence of HIN-1 expression through methylation of its gene promoter in gastric cancer

AIM: To clarify the role of high in normal-1 (HIN-1) gene promoter methylation during gastric cancer development. METHODS: Gastric cancer cell lines and tissue specimens were analyzed for expression of HIN-1 mRNA and protein using the semi-quantitative reverse transcription polymerase chain reaction and immunohistochemistry. The methylation of the HIN-1 gene promoter was detected in gastric carcinoma cells and tissues using methylation-specific polymerase chain reaction. The 3-(4,5-dimethylthiazol-2yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium cell viability assay and flow cytometry were used to assess the changes in behaviors of gastric cancer cells with or without 5-aza-2’-deoxycytidine treatment. RESULTS: HIN-1 was not expressed in 4 of 5 gastric cancer cell lines. The demethylation reagent 5-aza-2’-deoxycytidine was able to induce or upregulate HIN-1 expression in gastric cancer cell lines, which is associated with reduction of tumor cell viability. Furthermore, methylation of the HIN-1 gene promoter was shown in 57.8% (26/45) of the primary gastric cancer and 42.1% (17/38) of adjacent tissue samples, but was not shown in normal gastric mucosa (0/10). From the clinicopathological data of the patients, methylation of the HIN-1 gene promoter was found to be associated with tumor differentiation (P = 0.000). CONCLUSION: High methylation of HIN-1 gene promoter results in silence of HIN-1 expression in gastric cancer. 5-aza-2’-deoxycytidine reverses HIN-1 methylation and reduces viability of gastric cancer cells.

Opposed multi-burner gasification technology:Recent process of fundamental research and industrial application

Opposed multi-burner(OMB)gasification technology is the first large-scale gasification technology developed in China with completely independent intellectual property rights.It has been widely used around the world,involving synthetic ammonia,methanol,ethylene glycol,coal liquefaction,hydrogen production and other fields.This paper summarizes the research and development process of OMB gasification technology from the perspective of the cold model experiment and process simulation,pilotscale study and industrial demonstration.The latest progress of fundamental research in nozzle atomization and dispersion,mixing enhancement of impinging flow,multiscale reaction of different carbonaceous feedstocks,spectral characteristic of impinging flame and particle characteristics inside gasifier,and comprehensive gasification model are reviewed.The latest industrial application progress of ultralarge-scale OMB gasifier and radiant syngas cooler(RSC)combined with quenching chamber OMB gasifier are introduced,and the prospects for the future technical development are proposed as well.

息肉状脉络膜血管病变的光学相干断层扫描及血管成像的观察

目的:对比观察息肉状脉络膜血管病变(PCV)频域光相干断层成像(OCT)与光相干断层扫描血管成像(OCTA)的图像特征。方法:选取2018-03/12在我院确诊为息肉状脉络膜血管病变(PCV)的患者25例25眼纳入研究,所有患者均行频域OCT和OCTA检查,观察上述检查结果的图像特征。结果:本研究纳入患者25眼,频域OCT检查表现为浆液性和出血性色素上皮脱离(PED)14眼,OCTA检查示相应病变部位为低信号暗区,其旁有强信号点9眼;频域OCT检查表现为“双层征”11眼,OCTA检查显示为网状结构的脉络膜异常分支血管网(BVN);频域OCT检查表现为息肉样病变(polyps)12眼,OCTA检查表现为强或低信号区。结论:PCV患眼OCTA检查对BVN的表现更直观明显,息肉样病变表现为强或弱的信号,浆液性、出血性PED的OCT和OCTA图像均表现为低反射或低信号暗区,不具有特征性。二者显示的病变位置和形态具有相似性,但也有不同。

Immersed Interface Finite Element Methods for Elasticity Interface Problems with Non-Homogeneous Jump Conditions

In this paper,a class of new immersed interface finite element methods (IIFEM) is developed to solve elasticity interface problems with homogeneous and non-homogeneous jump conditions in two dimensions.Simple non-body-fitted meshes are used.For homogeneous jump conditions,both non-conforming and conforming basis functions are constructed in such a way that they satisfy the natural jump conditions. For non-homogeneous jump conditions,a pair of functions that satisfy the same non-homogeneous jump conditions are constructed using a level-set representation of the interface.With such a pair of functions,the discontinuities across the interface in the solution and flux are removed;and an equivalent elasticity interface problem with homogeneous jump conditions is formulated.Numerical examples are presented to demonstrate that such methods have second order convergence.

Structured illumination microscopy based on asymmetric three-beam interference

Structured illumination microscopy(SIM)is a rapidly developing super-resolution technology.It has been widely used in various application fields of biomedicine due to its excellent two-and three-dimensional imaging capabilities.Furthermore,faster three-dimensional imaging methods are required to help enable more research-oriented living cell imaging.In this paper,a fast and sensitive three-dimensional structured illumination microscopy based on asymmetric three-beam interference is proposed.An innovative time-series acquisition method is employed to halve the time required to obtain each raw image.A segmented half-wave plate as a substantial linear polarization modulation method is applied to the three-dimensional SIM system for the first time.Although it needs to acquire 21 raw images instead of 15 to reconstruct one super-resolution image,the SIM setup proposed in this paper is 30%faster than the traditional spatial light modulator-SIM(SLM-SIM)in imaging each super-resolution image.The related theoretical derivation,hardware system,and verification experiment are elaborated in this paper.The stable and fast 3D super-resolution imaging method proposed in this paper is of great significance to the research of organelle interaction,intercellular communication,and other biomedical fields.

Phenotype of Usher syndrome type Ⅱ assosiated with compound missense mutations of c.721 C>T and c.1969 C>T in MYO7A in a Chinese Usher syndrome family

·AIM: To identify the pathogenic mutations in a Chinese pedigree affected with Usher syndrome type II(USH2).· METHODS: The ophthalmic examinations and audiometric tests were performed to ascertain the phenotype of the family. To detect the genetic defect,exons of 103 known RDs-associated genes including 12 Usher syndrome(USH) genes of the proband were captured and sequencing analysis was performed to exclude known genetic defects and find potential pathogenic mutations. Subsequently, candidate mutations were validated in his pedigree and 100 normal controls using polymerase chain reaction(PCR) and Sanger sequencing.·RESULTS: The patient in the family occurred hearing loss(HL) and retinitis pigmentosa(RP) without vestibular dysfunction, which were consistent with standards of classification for USH2. He carried the compound heterozygous mutations, c.721 C 】T and c.1969 C 】T, in the MYO7 A gene and the unaffected members carried only one of the two mutations. The mutations were not present in the 100 normal controls.· CONCLUSION: We suggested that the compound heterozygous mutations of the MYO7 A could lead to USH2, which had revealed distinguished clinical phenotypes associated with MYO7 A and expanded the spectrum of clinical phenotypes of the MYO7 A mutations.

Effect of nintedanib thermo-sensitive hydrogel on neovascularization in alkali burn rat model

AIM:To investigate the effects of nintedanib thermo-sensitive hydrogel(NTH)on neovascularization and related markers in corneal alkali burns of Wistar rats.METHODS:NTH was prepared by grinding,and its phase-transition temperature was determined.Thirty specific-pathogen-free Wistar rats served as a model of corneal alkali burn in the right eye were randomly divided into 3 groups(n=10,each):model group treated with 0.9%saline once a day,NTH group with 0.2%nintedanib b.i.d,and dexamethasone group with dexamethasone ointment once a day.The left eye of rats served as the controls.The corneal transparency was observed under a slit-lamp microscope,and the area of neovascularization was calculated.On day 7,the rats were sacrificed,and the cornea was removed and embedded with paraffin,then stained with hematoxylin一eosin,and the expression of vascular endothelial growth factor receptor 2(VEGFR-2)and CD31 in the corneal tissues of each group was detected by immunofluorescence.RESULTS:The phase-transition temperature ofnintedanib obtained by grinding was 37℃after adding artificial tears.The results of the alkali burn model indicated that the growth rate of neovascularization in the NTH group was slower than that in the model group,and the neovascularization area was significantly smaller than that in the model group(P<0.05).Moreover,CD31 and VEGFR-2 expression levels in the NTH group were significantly lower than those in the model group.CONCLUSION:NTH becomes colloidal at body temperature,which is beneficial for releasing the drug slowly and can significantly inhibit the neovascularization of corneal induced by alkali burn in rats.

TaNAC48 positively regulates drought tolerance and ABA responses in wheat(Triticum aestivum L.)

NAC family transcription factors(TFs) are important regulators in plant development and stress responses. However, the biological functions of NAC TFs in wheat are rarely studied. In this study, 43 putative drought-induced NAC genes were identified from de novo transcriptome sequencing data of wheat following drought treatment. Twelve wheat NACs along with ten known stress-related NACs from Arabidopsis and rice were clustered into Group II based on a phylogenetic analysis. Ta NAC48, which showed a higher and constitutive expression level in Group Ⅱ, was selected for further investigation.Ta NAC48 transcript was up-regulated by drought, PEG, H_(2)O_(2) and abscisic acid(ABA) treatment and encoded a nuclear localized protein. Overexpression of Ta NAC48 significantly promoted drought tolerance with increased proline content, and decreased rates of water loss, malondialdehyde(MDA), H_(2)O_(2) and O_(2)^(-) content. Root length and a stomatal aperture assay confirmed that Ta NAC48-overexpression plants increased sensitivity to ABA. Electrophoretic mobility shift assay(EMSA) and luciferase reporter analysis indicated that Ta AREB3 could bind to a cis-acting ABA-responsive element(ABRE) on Ta NAC48 promoter and activate the expression of Ta NAC48. These results suggest that Ta NAC48 is essential in mediating crosstalk between the ABA signaling pathway and drought stress responses in wheat.

装配式住宅建筑给排水管线的设计探讨

我国经济发展离不开建筑行业的贡献,随着人民群众生活水平逐年提升,广大人民群众迫切希望改善住房条件,因此给建筑行业带来了广阔的发展机遇。对于建筑工程行业而言,高效地完成建筑工程建设工作,不仅符合当下建筑行业发展要求,而且有助于促进建筑经济获得长久发展动力,装配式住宅建筑也就因此应运而生。在装配式住宅建筑中,给排水管线设计无疑是整个建筑工程的设计要点,所以本文将对装配式住宅建筑给排水管线的设计进行深入探析,以供广大专业人士借鉴与学习。

Electrically Tunable Wafer-Sized Three-Dimensional Topological Insulator Thin Films Grown by Magnetron Sputtering

Three-dimensional(3 D)topological insulators(TIs)are candidate materials for various electronic and spintronic devices due to their strong spin-orbit coupling and unique surface electronic structure.Rapid,low-cost preparation of large-area TI thin films compatible with conventional semiconductor technology is the key to the practical applications of TIs.Here we show that wafer-sized Bi2Te3 family TI and magnetic TI films with decent quality and well-controlled composition and properties can be prepared on amorphous SiO2/Si substrates by magnetron cosputtering.The SiO2/Si substrates enable us to electrically tune(Bi1-xSbx)2Te3 and Cr-doped(Bi1-xSbx)2 Te3 TI films between p-type and n-type behavior and thus study the phenomena associated with topological surface states,such as the quantum anomalous Hall effect(QAHE).This work significantly facilitates the fabrication of TI-based devices for electronic and spintronic applications.