BACKGROUND Perihilar cholangiocarcinoma(pCCA)has a poor prognosis and urgently needs a better predictive method.The predictive value of the age-adjusted Charlson comorbidity index(ACCI)for the long-term prognosis of p...BACKGROUND Perihilar cholangiocarcinoma(pCCA)has a poor prognosis and urgently needs a better predictive method.The predictive value of the age-adjusted Charlson comorbidity index(ACCI)for the long-term prognosis of patients with multiple malignancies was recently reported.However,pCCA is one of the most surgically difficult gastrointestinal tumors with the poorest prognosis,and the value of the ACCI for the prognosis of pCCA patients after curative resection is unclear.AIM To evaluate the prognostic value of the ACCI and to design an online clinical model for pCCA patients.METHODS Consecutive pCCA patients after curative resection between 2010 and 2019 were enrolled from a multicenter database.The patients were randomly assigned 3:1 to training and validation cohorts.In the training and validation cohorts,all patients were divided into low-,moderate-,and high-ACCI groups.Kaplan-Meier curves were used to determine the impact of the ACCI on overall survival(OS)for pCCA patients,and multivariate Cox regression analysis was used to determine the independent risk factors affecting OS.An online clinical model based on the ACCI was developed and validated.The concordance index(C-index),calibration curve,and receiver operating characteristic(ROC)curve were used to evaluate the predictive performance and fit of this model.RESULTS A total of 325 patients were included.There were 244 patients in the training cohort and 81 patients in the validation cohort.In the training cohort,116,91 and 37 patients were classified into the low-,moderate-and high-ACCI groups.The Kaplan-Meier curves showed that patients in the moderate-and high-ACCI groups had worse survival rates than those in the low-ACCI group.Multivariable analysis revealed that moderate and high ACCI scores were independently associated with OS in pCCA patients after curative resection.In addition,an online clinical model was developed that had ideal C-indexes of 0.725 and 0.675 for predicting OS in the training and validation cohorts.The calibration curve and ROC curve indicated that the model had a good fit and prediction performance.CONCLUSION A high ACCI score may predict poor long-term survival in pCCA patients after curative resection.High-risk patients screened by the ACCI-based model should be given more clinical attention in terms of the management of comorbidities and postoperative follow-up.展开更多
3D reconstruction based on single view aims to reconstruct the entire 3D shape of an object from one perspective.When existing methods reconstruct the mesh surface of complex objects,the surface details are difficult ...3D reconstruction based on single view aims to reconstruct the entire 3D shape of an object from one perspective.When existing methods reconstruct the mesh surface of complex objects,the surface details are difficult to predict and the reconstruction visual effect is poor because the mesh representation is not easily integrated into the deep learning framework;the 3D topology is easily limited by predefined templates and inflexible,and unnecessary mesh self-intersections and connections will be generated when reconstructing complex topology,thus destroying the surface details;the training of the reconstruction network is limited by the large amount of information attached to the mesh vertices,and the training time of the reconstructed network is too long.In this paper,we propose a method for fast mesh reconstruction from single view based on Graph Convolutional Network(GCN)and topology modification.We use GCN to ensure the generation of high-quality mesh surfaces and use topology modification to improve the flexibility of the topology.Meanwhile,a feature fusion method is proposed to make full use of the features of each stage of the image hierarchically.We use 3D open dataset ShapeNet to train our network and add a new weight parameter to speed up the training process.Extensive experiments demonstrate that our method can not only reconstruct object meshes on complex topological surfaces,but also has better qualitative and quantitative results.展开更多
The principal moments of inertia(PMIs)with the principal axes are usually taken as the dynamic figure parameters of Mars;they can be deduced from satellite-observed degree-two gravitational potentials in recent global...The principal moments of inertia(PMIs)with the principal axes are usually taken as the dynamic figure parameters of Mars;they can be deduced from satellite-observed degree-two gravitational potentials in recent global gravity models and from the dynamic ellipticities resulting from precession observations.These PMIs are natural and significant for the geodetic,geophysical,and geodynamic problems of Mars,which are functions of internal density distributions.In this study,a closed and concise formula for determining the PMIs of the entire planet and its core was developed based on the second invariants of gravity and a multipole expansion.We deduced the polar oblateness J^(2)and the equatorial ellipticity J_(22)of Mars to be 1.9566×10^(−3)and 6.3106×10^(−5),respectively.The preferred principal moments of inertia of Mars are A=2.66589×1036 kg·m^(2),B=2.66775×10^(36)kg·m^(2),and C=2.68125×10^(36)kg·m^(2).These values indicate that Mar is slightly triaxial.The equatorial principal moment of inertia of the Martian core is 1.46008×10^(35)kg·m^(2),accounting for~5.47%of the planet’s PMI;this result is critical for investigating the density and size of the core of Mars,and the planet’s free core nutation.展开更多
A high order finite difference numerical scheme is developed for the shallow water equations on curvilinear meshes based on an alternative flux formulation of the weighted essentially non-oscillatory(WENO)scheme.The e...A high order finite difference numerical scheme is developed for the shallow water equations on curvilinear meshes based on an alternative flux formulation of the weighted essentially non-oscillatory(WENO)scheme.The exact C-property is investigated,and comparison with the standard finite difference WENO scheme is made.Theoretical derivation and numerical results show that the proposed finite difference WENO scheme can maintain the exact C-property on both stationarily and dynamically generalized coordinate systems.The Harten-Lax-van Leer type flux is developed on general curvilinear meshes in two dimensions and verified on a number of benchmark problems,indicating smaller errors compared with the Lax-Friedrichs solver.In addition,we propose a positivity-preserving limiter on stationary meshes such that the scheme can preserve the non-negativity of the water height without loss of mass conservation.展开更多
Introduction:Allergen-specific CD4+T cells play a central role in autoimmune disorders,allergies and asthma,with Th2-type immunity being the typical functional response of CD4+T cells.This study aimed to investigate t...Introduction:Allergen-specific CD4+T cells play a central role in autoimmune disorders,allergies and asthma,with Th2-type immunity being the typical functional response of CD4+T cells.This study aimed to investigate the role of MBD2 in regulating Th2 cell differentiation.Methods:Splenic mononuclear cells were extracted from C57BL/6 mice,and CD4+T cells were isolated using magnetic beads and confirmed through flow cytometry.Lentivirus was employed to construct MBD2-silenced CD4+T cells.In vitro experiments were performed to treat splenogenic mononuclear cells and CD4+T cells with Ovalbumin(OVA),and Th2 cell ratios and IL-4 levels were assessed using flow cytometry and ELISA.Results:The purity of the isolated CD4+T cells was 95.73%,confirming successful isolation of primary CD4+T cells.Compared to the control group,the Th2 cell ratio exhibited an increase in the Th2-induced group.Treatment with 5-Aza(concentrations,1-100μM)promoted Th2 cell differentiation and increased IL-4 levels.Notably,when combined with Th2 induction and 10μM 5-Aza treatment,silencing MBD2 further amplified Th2 cell ratios and elevated IL-4 levels in cell supernatants.Furthermore,OVA(concentration,200μg/mL)induced the differentiation of CD4+T cells into Th2 cells and increased IL-4 secretion.Interestingly,silencing MBD2 significantly increased the Th2 cell ratio and IL-4 levels in OVA-treated CD4+T cells.Conclusion:In summary,OVA promoted CD4+T cell differentiation into Th2 cells and enhanced IL-4 levels.MBD2 was identified as a mediator of Th2 cell differentiation in splenic-derived CD4+T cells,influenced by OVA or 5-Aza treatment.展开更多
Introduction:Structural maintenance of chromosome 1A(SMC1A)is a crucial compound of the cohesin complex.It has been reported to regulate the epithelial-mesenchymal transition(EMT)process in multiple cancers.Objectives...Introduction:Structural maintenance of chromosome 1A(SMC1A)is a crucial compound of the cohesin complex.It has been reported to regulate the epithelial-mesenchymal transition(EMT)process in multiple cancers.Objectives:The present study aims to further clarify the role of SMC1A in cervical cancer.Methods:We analyzed data from four datasets and confirmed that SMC1A showed high expression in cervical cancer samples and was related to poor prognosis of patients with cervical cancer.Cell proliferation of SiHa and C-33A with knockdown of SMC1A was assessed using CCK-8 and colony formation assay.The migration and invasion were estimated by wound healing assay and Transwell assay separately.The effect of SMC1A on the chemosensitivity of cisplatin and paclitaxel in cervical cancer cells was detected by flow cytometry assay.Results:Results of the immunohistochemistry(IHC)assay confirmed that the expression of SMC1A was increased in tumor tissues.The cell viability was remarkably suppressed in SiHa and C-33A by knocking down the expression of SMC1A.The increase of E-cadherin expression and decrease of N-cadherin and Snail expression verified that inhibition of SMC1A suppressed the EMT process of cervical cancer cells.Further,cell migration,and invasion were significantly repressed by the absence of SMC1A.Cisplatin and paclitaxel are effective chemotherapeutic agents used in the treatment of cervical cancer.Silencing of SMC1A remarkably promoted the apoptosis induced by cisplatin and paclitaxel,revealing that the chemotherapy resistance to cisplatin and paclitaxel in cervical cancer could reduce by knocking down SMC1A.Further,metastasis associated with colon cancer 1(MACC1)was identified as the downstream factor of SMC1A.Its upregulation reversed the proliferation and the EMT process induced by SMC1A silencing.Conclusion:Therefore,our study concluded that SMC1A serves as a therapeutic molecular target to regulate the malignant phenotypes of cervical cancer.展开更多
Pollination biology studies of the endangered herbal medicines Dendrobium chrysotoxum were conducted in natural pollination conditions using flower observation,pollinator observation and artificial pollination experim...Pollination biology studies of the endangered herbal medicines Dendrobium chrysotoxum were conducted in natural pollination conditions using flower observation,pollinator observation and artificial pollination experiments.Populations of D.chrysotoxum with fragrance and nectar were pollinated by Ctenoplectra davidi Valhalla(Hymenoptera:Apidae)species.The floral structure of D.chrysotoxum adapted precisely to its pollinators.Flowers had a low capsule setting(0.17%)under natural conditions.However,compared to open pollination,artificial pollination experiments showed a significant increase in capsule setting,and D.chrysotoxum was cross-compatible and self-compatible,but there was pollinator limitation also.This study will provide important information for the preservation of this endangered species.展开更多
BACKGROUND With the recent improvement of endoscopic techniques,endoscopic ultrasoundguided fine needle aspiration and endoscopic submucosal tunnel dissection(ESTD)have been widely used for accurate diagnosis and diss...BACKGROUND With the recent improvement of endoscopic techniques,endoscopic ultrasoundguided fine needle aspiration and endoscopic submucosal tunnel dissection(ESTD)have been widely used for accurate diagnosis and dissection acceleration of esophageal tumors.CASE SUMMARY We used a modified submucosal tunnel technique during endoscopic en bloc resection in a 58-year-old man with large esophageal submucosal gland duct adenoma(ESGDA).During modified ESTD,the oral end of the involved mucosa was cut transversely,followed by a submucosal tunnel created from the proximal to the distal end,and the anal end of the involved mucosa blocked by the tumor was incised.As a result of retaining submucosal injection solutions using the submucosal tunnel technique,it was possible to reduce the amount of injection required and increase the efficiency and safety of dissection.CONCLUSION Modified ESTD is an effective treatment strategy for large ESGDAs.Single-tunnel ESTD appears to be a time-saving procedure compared with conventional endoscopic submucosal dissection.展开更多
基金Supported by National Natural Science Foundation of China,No. 81874211Chongqing Technology Innovation and Application Development Special Key Project,No. CSTC2021jscx-gksb-N0009
文摘BACKGROUND Perihilar cholangiocarcinoma(pCCA)has a poor prognosis and urgently needs a better predictive method.The predictive value of the age-adjusted Charlson comorbidity index(ACCI)for the long-term prognosis of patients with multiple malignancies was recently reported.However,pCCA is one of the most surgically difficult gastrointestinal tumors with the poorest prognosis,and the value of the ACCI for the prognosis of pCCA patients after curative resection is unclear.AIM To evaluate the prognostic value of the ACCI and to design an online clinical model for pCCA patients.METHODS Consecutive pCCA patients after curative resection between 2010 and 2019 were enrolled from a multicenter database.The patients were randomly assigned 3:1 to training and validation cohorts.In the training and validation cohorts,all patients were divided into low-,moderate-,and high-ACCI groups.Kaplan-Meier curves were used to determine the impact of the ACCI on overall survival(OS)for pCCA patients,and multivariate Cox regression analysis was used to determine the independent risk factors affecting OS.An online clinical model based on the ACCI was developed and validated.The concordance index(C-index),calibration curve,and receiver operating characteristic(ROC)curve were used to evaluate the predictive performance and fit of this model.RESULTS A total of 325 patients were included.There were 244 patients in the training cohort and 81 patients in the validation cohort.In the training cohort,116,91 and 37 patients were classified into the low-,moderate-and high-ACCI groups.The Kaplan-Meier curves showed that patients in the moderate-and high-ACCI groups had worse survival rates than those in the low-ACCI group.Multivariable analysis revealed that moderate and high ACCI scores were independently associated with OS in pCCA patients after curative resection.In addition,an online clinical model was developed that had ideal C-indexes of 0.725 and 0.675 for predicting OS in the training and validation cohorts.The calibration curve and ROC curve indicated that the model had a good fit and prediction performance.CONCLUSION A high ACCI score may predict poor long-term survival in pCCA patients after curative resection.High-risk patients screened by the ACCI-based model should be given more clinical attention in terms of the management of comorbidities and postoperative follow-up.
基金This work was supported,in part,by the Natural Science Foundation of Jiangsu Province under Grant Numbers BK20201136,BK20191401in part,by the National Nature Science Foundation of China under Grant Numbers 61502240,61502096,61304205,61773219in part,by the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)fund.
文摘3D reconstruction based on single view aims to reconstruct the entire 3D shape of an object from one perspective.When existing methods reconstruct the mesh surface of complex objects,the surface details are difficult to predict and the reconstruction visual effect is poor because the mesh representation is not easily integrated into the deep learning framework;the 3D topology is easily limited by predefined templates and inflexible,and unnecessary mesh self-intersections and connections will be generated when reconstructing complex topology,thus destroying the surface details;the training of the reconstruction network is limited by the large amount of information attached to the mesh vertices,and the training time of the reconstructed network is too long.In this paper,we propose a method for fast mesh reconstruction from single view based on Graph Convolutional Network(GCN)and topology modification.We use GCN to ensure the generation of high-quality mesh surfaces and use topology modification to improve the flexibility of the topology.Meanwhile,a feature fusion method is proposed to make full use of the features of each stage of the image hierarchically.We use 3D open dataset ShapeNet to train our network and add a new weight parameter to speed up the training process.Extensive experiments demonstrate that our method can not only reconstruct object meshes on complex topological surfaces,but also has better qualitative and quantitative results.
基金supported by the National Key Research and Development Program (2022YFF0503200)the National Natural Science Foundation of China (42274114)the Key Program of the Institute of Geology and Geophysics, Chinese Academy of Sciences (IGGCAS-202102)
文摘The principal moments of inertia(PMIs)with the principal axes are usually taken as the dynamic figure parameters of Mars;they can be deduced from satellite-observed degree-two gravitational potentials in recent global gravity models and from the dynamic ellipticities resulting from precession observations.These PMIs are natural and significant for the geodetic,geophysical,and geodynamic problems of Mars,which are functions of internal density distributions.In this study,a closed and concise formula for determining the PMIs of the entire planet and its core was developed based on the second invariants of gravity and a multipole expansion.We deduced the polar oblateness J^(2)and the equatorial ellipticity J_(22)of Mars to be 1.9566×10^(−3)and 6.3106×10^(−5),respectively.The preferred principal moments of inertia of Mars are A=2.66589×1036 kg·m^(2),B=2.66775×10^(36)kg·m^(2),and C=2.68125×10^(36)kg·m^(2).These values indicate that Mar is slightly triaxial.The equatorial principal moment of inertia of the Martian core is 1.46008×10^(35)kg·m^(2),accounting for~5.47%of the planet’s PMI;this result is critical for investigating the density and size of the core of Mars,and the planet’s free core nutation.
基金the National Natural Science Foundation of China(11901555,11871448,12001009).
文摘A high order finite difference numerical scheme is developed for the shallow water equations on curvilinear meshes based on an alternative flux formulation of the weighted essentially non-oscillatory(WENO)scheme.The exact C-property is investigated,and comparison with the standard finite difference WENO scheme is made.Theoretical derivation and numerical results show that the proposed finite difference WENO scheme can maintain the exact C-property on both stationarily and dynamically generalized coordinate systems.The Harten-Lax-van Leer type flux is developed on general curvilinear meshes in two dimensions and verified on a number of benchmark problems,indicating smaller errors compared with the Lax-Friedrichs solver.In addition,we propose a positivity-preserving limiter on stationary meshes such that the scheme can preserve the non-negativity of the water height without loss of mass conservation.
基金supported by grants from the National Natural Science Foundation of China(Nos.81760009 and 81560007).
文摘Introduction:Allergen-specific CD4+T cells play a central role in autoimmune disorders,allergies and asthma,with Th2-type immunity being the typical functional response of CD4+T cells.This study aimed to investigate the role of MBD2 in regulating Th2 cell differentiation.Methods:Splenic mononuclear cells were extracted from C57BL/6 mice,and CD4+T cells were isolated using magnetic beads and confirmed through flow cytometry.Lentivirus was employed to construct MBD2-silenced CD4+T cells.In vitro experiments were performed to treat splenogenic mononuclear cells and CD4+T cells with Ovalbumin(OVA),and Th2 cell ratios and IL-4 levels were assessed using flow cytometry and ELISA.Results:The purity of the isolated CD4+T cells was 95.73%,confirming successful isolation of primary CD4+T cells.Compared to the control group,the Th2 cell ratio exhibited an increase in the Th2-induced group.Treatment with 5-Aza(concentrations,1-100μM)promoted Th2 cell differentiation and increased IL-4 levels.Notably,when combined with Th2 induction and 10μM 5-Aza treatment,silencing MBD2 further amplified Th2 cell ratios and elevated IL-4 levels in cell supernatants.Furthermore,OVA(concentration,200μg/mL)induced the differentiation of CD4+T cells into Th2 cells and increased IL-4 secretion.Interestingly,silencing MBD2 significantly increased the Th2 cell ratio and IL-4 levels in OVA-treated CD4+T cells.Conclusion:In summary,OVA promoted CD4+T cell differentiation into Th2 cells and enhanced IL-4 levels.MBD2 was identified as a mediator of Th2 cell differentiation in splenic-derived CD4+T cells,influenced by OVA or 5-Aza treatment.
文摘Introduction:Structural maintenance of chromosome 1A(SMC1A)is a crucial compound of the cohesin complex.It has been reported to regulate the epithelial-mesenchymal transition(EMT)process in multiple cancers.Objectives:The present study aims to further clarify the role of SMC1A in cervical cancer.Methods:We analyzed data from four datasets and confirmed that SMC1A showed high expression in cervical cancer samples and was related to poor prognosis of patients with cervical cancer.Cell proliferation of SiHa and C-33A with knockdown of SMC1A was assessed using CCK-8 and colony formation assay.The migration and invasion were estimated by wound healing assay and Transwell assay separately.The effect of SMC1A on the chemosensitivity of cisplatin and paclitaxel in cervical cancer cells was detected by flow cytometry assay.Results:Results of the immunohistochemistry(IHC)assay confirmed that the expression of SMC1A was increased in tumor tissues.The cell viability was remarkably suppressed in SiHa and C-33A by knocking down the expression of SMC1A.The increase of E-cadherin expression and decrease of N-cadherin and Snail expression verified that inhibition of SMC1A suppressed the EMT process of cervical cancer cells.Further,cell migration,and invasion were significantly repressed by the absence of SMC1A.Cisplatin and paclitaxel are effective chemotherapeutic agents used in the treatment of cervical cancer.Silencing of SMC1A remarkably promoted the apoptosis induced by cisplatin and paclitaxel,revealing that the chemotherapy resistance to cisplatin and paclitaxel in cervical cancer could reduce by knocking down SMC1A.Further,metastasis associated with colon cancer 1(MACC1)was identified as the downstream factor of SMC1A.Its upregulation reversed the proliferation and the EMT process induced by SMC1A silencing.Conclusion:Therefore,our study concluded that SMC1A serves as a therapeutic molecular target to regulate the malignant phenotypes of cervical cancer.
基金funded by the Basic Research Priorities Program of Yunnan Provincethe Applied Basic Research Programs of Science and Technology Department for Young Scholars(No.2019FD005)Technological Innovation Talents Cultivation Project of Yunnan Province(No.202205AD160043)Technological Innovation Talents Cultivation Project of Dehong City(No.2021RC007).
文摘Pollination biology studies of the endangered herbal medicines Dendrobium chrysotoxum were conducted in natural pollination conditions using flower observation,pollinator observation and artificial pollination experiments.Populations of D.chrysotoxum with fragrance and nectar were pollinated by Ctenoplectra davidi Valhalla(Hymenoptera:Apidae)species.The floral structure of D.chrysotoxum adapted precisely to its pollinators.Flowers had a low capsule setting(0.17%)under natural conditions.However,compared to open pollination,artificial pollination experiments showed a significant increase in capsule setting,and D.chrysotoxum was cross-compatible and self-compatible,but there was pollinator limitation also.This study will provide important information for the preservation of this endangered species.
基金Supported by Young and Middle-aged Mainstay Talent Training Program of Fujian Provincial Health System,China,No. 2017-ZQN-16
文摘BACKGROUND With the recent improvement of endoscopic techniques,endoscopic ultrasoundguided fine needle aspiration and endoscopic submucosal tunnel dissection(ESTD)have been widely used for accurate diagnosis and dissection acceleration of esophageal tumors.CASE SUMMARY We used a modified submucosal tunnel technique during endoscopic en bloc resection in a 58-year-old man with large esophageal submucosal gland duct adenoma(ESGDA).During modified ESTD,the oral end of the involved mucosa was cut transversely,followed by a submucosal tunnel created from the proximal to the distal end,and the anal end of the involved mucosa blocked by the tumor was incised.As a result of retaining submucosal injection solutions using the submucosal tunnel technique,it was possible to reduce the amount of injection required and increase the efficiency and safety of dissection.CONCLUSION Modified ESTD is an effective treatment strategy for large ESGDAs.Single-tunnel ESTD appears to be a time-saving procedure compared with conventional endoscopic submucosal dissection.
