The phase diagram of a cetyltrimethyl ammonium bromide （CTAB）/n-butanol/n-octane/KNO3-Mg（ NO3）2 system was drawn. Nanoparticles of Eu^2＋ -doped KMgF3 were prepared from the quaternary microemulsions of cetyltrime...The phase diagram of a cetyltrimethyl ammonium bromide （CTAB）/n-butanol/n-octane/KNO3-Mg（ NO3）2 system was drawn. Nanoparticles of Eu^2＋ -doped KMgF3 were prepared from the quaternary microemulsions of cetyltrimethyl ammonium bromide（ CTAB）, a-butanol, n-octane and water. The X-ray diffraction（XRD） patterns were indexed to a pure KMgF3 cubic phase. The environmental scanning electron microscopic（ESEM） images show the presence of spherical Eu^2＋ -doped KMgF3 nanoparticles with a diameter of ca. 20 nm. The emission of KMgF3： Eu^2＋ nanoparticles peaks at 360 nm. The excitation band was observed at 250 nm with a blue shift of ca. 70 nm compared with that of KMgF3： Eu^2＋ single crystal. The preparation method of nano-KMgF3： Eu^2＋/PMMA composite films was inquired into.展开更多
Precipitation regimes of China were mapped by using empirical orthogonal function (EOF) analysis with orthogonal rotation based on normalized monthly mean precipitation data at 400 stations from 1961 to 2006.The regio...Precipitation regimes of China were mapped by using empirical orthogonal function (EOF) analysis with orthogonal rotation based on normalized monthly mean precipitation data at 400 stations from 1961 to 2006.The regional boundaries were defined with the maximum-loading approach.The results identified 13 consistent and contiguous precipitation regions with distinct regional variations in China that are expected to reflect dominant regional patterns focusing on seasonal and interannual precipitation variations.The precipitation regionalization is operationally useful for seasonal and interannual climate predictions.展开更多
Nanocrystals of KMgF3 single-doped and codoped with Ce^3＋ or/and Yb^3＋ were synthesized separately by the mi-croemulsion method. The X-ray diffraction（XRD） patterns were indexed to show that the KMgF3 crystal syst...Nanocrystals of KMgF3 single-doped and codoped with Ce^3＋ or/and Yb^3＋ were synthesized separately by the mi-croemulsion method. The X-ray diffraction（XRD） patterns were indexed to show that the KMgF3 crystal system was unchanged. The fluorescent spectra of KMgF3： Ce, Yb polycrystal powders were studied and compared with those of the Ce,Yb doped KMgF3 crystals produced using the high-temperature solid phase method. The diffuse reflection spectra and infrared emission of KMgF3： Ce, Yb were investigated. From the results, the authors could confirm that there were charge transfer processes from Ce^3＋ to Yb^3＋ in both KMgF3： Ce,Yb nanocrystals and polycrystal powders.展开更多
Soft rot caused by Pectobacterium carotovorum(Pc)is a devastating disease of Brassica rapa,causing substantial reductions in crop yield and quality.Identifying genes related to soft rot resistance is the key to solvin...Soft rot caused by Pectobacterium carotovorum(Pc)is a devastating disease of Brassica rapa,causing substantial reductions in crop yield and quality.Identifying genes related to soft rot resistance is the key to solving this problem.To characterize soft rot resistance,we screened a soft rot-susceptible Chinese cabbage(A03),a resistant pakchoi(‘Huaguan’),and a resistant mutant(sr).An F_(2) population was generated by crossing susceptible Chinese cabbage A03and resistant pakchoi‘Huaguan’to identify quantitative trait loci(QTLs)that confer soft rot resistance.A high-density genetic map was constructed and the three QTLs identified contain 166 genes.Based on available transcriptome data,we analyzed the expression of the 166 genes during an important defense regulatory period in Pc infection in both A03and the resistant mutant sr.Among the 166 genes,six candidate genes were related to the soft rot defense response in B.rapa.TIFY10B(JAZ2,BraA07g038660.3C)was located in the major soft rot resistance QTL,DRQTL-3 on A07,and we speculate that this gene may play an important role in the defense mechanism against soft rot in B.rapa.This study lays the foundation for further investigations on the mechanism of soft rot resistance in B.rapa crops.展开更多
[ Objective] To prepare specific monoclonal antibodies against salbutamol （SAL） and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains （SA...[ Objective] To prepare specific monoclonal antibodies against salbutamol （SAL） and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains （SAL rnAb） secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [Resalt] Two hybridoma strains, SAL-2D9 and SAL-1C4, were established, and the indirect ELISA titers of the cell supematant and ascites were 1：1 × 10^4 and 1 ： 1 × 10^6, respectively. Their isotype was IgGl. The median inhibitory concentration （IC5o） of the SAL mAb against SAL was 1.14 ng/ml. It had cross-reactivity of 100% against SAL and 26.09% against clenobuterol hydrochloride, and no cross-reaction was found between the SAL mAb and other SAL analogues. [ Conclusion] The high-titer, sensitive and specific anti-SAL monoclonal antibodies have been produced, which makes it possible to develop immunoassay for detection of SAL residues.展开更多
文摘The phase diagram of a cetyltrimethyl ammonium bromide （CTAB）/n-butanol/n-octane/KNO3-Mg（ NO3）2 system was drawn. Nanoparticles of Eu^2＋ -doped KMgF3 were prepared from the quaternary microemulsions of cetyltrimethyl ammonium bromide（ CTAB）, a-butanol, n-octane and water. The X-ray diffraction（XRD） patterns were indexed to a pure KMgF3 cubic phase. The environmental scanning electron microscopic（ESEM） images show the presence of spherical Eu^2＋ -doped KMgF3 nanoparticles with a diameter of ca. 20 nm. The emission of KMgF3： Eu^2＋ nanoparticles peaks at 360 nm. The excitation band was observed at 250 nm with a blue shift of ca. 70 nm compared with that of KMgF3： Eu^2＋ single crystal. The preparation method of nano-KMgF3： Eu^2＋/PMMA composite films was inquired into.
基金jointly supported by the National Key Technologies R&D Program of China (2007BAC 29B04 and 2009BAC51B05)the China Meteorological Administration Operation Project (the Operational System Setup of Seasonal Climate Prediction)
文摘Precipitation regimes of China were mapped by using empirical orthogonal function (EOF) analysis with orthogonal rotation based on normalized monthly mean precipitation data at 400 stations from 1961 to 2006.The regional boundaries were defined with the maximum-loading approach.The results identified 13 consistent and contiguous precipitation regions with distinct regional variations in China that are expected to reflect dominant regional patterns focusing on seasonal and interannual precipitation variations.The precipitation regionalization is operationally useful for seasonal and interannual climate predictions.
基金Supported by the Science Foundation of Changchun City of China(No.2005093)the Science and Technology Departmentof Jilin Province,P.R.China(No.20050507).
文摘Nanocrystals of KMgF3 single-doped and codoped with Ce^3＋ or/and Yb^3＋ were synthesized separately by the mi-croemulsion method. The X-ray diffraction（XRD） patterns were indexed to show that the KMgF3 crystal system was unchanged. The fluorescent spectra of KMgF3： Ce, Yb polycrystal powders were studied and compared with those of the Ce,Yb doped KMgF3 crystals produced using the high-temperature solid phase method. The diffuse reflection spectra and infrared emission of KMgF3： Ce, Yb were investigated. From the results, the authors could confirm that there were charge transfer processes from Ce^3＋ to Yb^3＋ in both KMgF3： Ce,Yb nanocrystals and polycrystal powders.
基金supported by the National Natural Science Foundation of China(31672151 and 31902005)the China Postdoctoral Science Foundation(2019M651059)+4 种基金the Natural Science Foundation of Hebei,China(C2020204111)the International Cooperation Project in the Science and Technology Support Program of Hebei,China(2019YX023A)the International Cooperation Base Project in the Technology of Hebei,China(20592901D)100 Foreign Experts Plan of Hebei Province and the Starting Grant from Hebei Agricultural University,China(YJ201955)the Science and Technology Research Project of Universities in Hebei Province,China(QN2021074)。
文摘Soft rot caused by Pectobacterium carotovorum(Pc)is a devastating disease of Brassica rapa,causing substantial reductions in crop yield and quality.Identifying genes related to soft rot resistance is the key to solving this problem.To characterize soft rot resistance,we screened a soft rot-susceptible Chinese cabbage(A03),a resistant pakchoi(‘Huaguan’),and a resistant mutant(sr).An F_(2) population was generated by crossing susceptible Chinese cabbage A03and resistant pakchoi‘Huaguan’to identify quantitative trait loci(QTLs)that confer soft rot resistance.A high-density genetic map was constructed and the three QTLs identified contain 166 genes.Based on available transcriptome data,we analyzed the expression of the 166 genes during an important defense regulatory period in Pc infection in both A03and the resistant mutant sr.Among the 166 genes,six candidate genes were related to the soft rot defense response in B.rapa.TIFY10B(JAZ2,BraA07g038660.3C)was located in the major soft rot resistance QTL,DRQTL-3 on A07,and we speculate that this gene may play an important role in the defense mechanism against soft rot in B.rapa.This study lays the foundation for further investigations on the mechanism of soft rot resistance in B.rapa crops.
基金funded by the Key Technology R&D Program of Hebei Province (09227134D)Key Technology R&D Program of Shijiazhuang City (10150142A)
文摘[ Objective] To prepare specific monoclonal antibodies against salbutamol （SAL） and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains （SAL rnAb） secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [Resalt] Two hybridoma strains, SAL-2D9 and SAL-1C4, were established, and the indirect ELISA titers of the cell supematant and ascites were 1：1 × 10^4 and 1 ： 1 × 10^6, respectively. Their isotype was IgGl. The median inhibitory concentration （IC5o） of the SAL mAb against SAL was 1.14 ng/ml. It had cross-reactivity of 100% against SAL and 26.09% against clenobuterol hydrochloride, and no cross-reaction was found between the SAL mAb and other SAL analogues. [ Conclusion] The high-titer, sensitive and specific anti-SAL monoclonal antibodies have been produced, which makes it possible to develop immunoassay for detection of SAL residues.
