缺氧诱导基因1C在牦牛隐睾中的表达及其调控机制研究

旨在探究缺氧诱导基因1C(HIG1 hypoxia inducible domain family member 1C,HIGD1C)对牦牛睾丸支持细胞凋亡的影响。本研究分别选择3头3~6岁龄健康状况良好的公牦牛和隐睾症公牦牛,以其睾丸组织作为研究材料。通过HE染色、牦牛睾丸支持细胞分离培养、间接免疫荧光、RT-qPCR、Western blot、HIGD1C过表达与干扰载体构建及体外细胞转染等技术,探究HIGD1C对牦牛睾丸支持细胞凋亡的调控机制。结果显示:正常睾丸基底膜及间质组织完整,曲细精管、管周肌细胞及支持细胞等排列紧密,隐睾中曲细精管断裂萎缩且HIGD1C在牦牛隐睾组织中转录及蛋白的表达水平极显著于高于正常睾丸组织(P<0.01);HIGD1C蛋白主要分布在睾丸间质细胞和支持细胞;成功分离培养出牦牛睾丸支持细胞且HIGD1C蛋白定位于原代睾丸支持细胞胞核;HIGD1C过表达支持细胞后,Bcl-2 mRNA表达水平显著上调(P<0.05),蛋白表达水平极显著上调(P<0.01),Bax和Caspase-3 mRNA和蛋白表达水平均极显著上调(P<0.01),流式细胞术结果显示过表达HIGD1C组支持细胞凋亡率极显著上升(P<0.01);反之,HIGD1C敲除后,Bax、Bcl-2和Caspase-3 mRNA和蛋白表达水平均极显著下调(P<0.01),流式细胞术结果显示HIGD1C敲除组支持细胞凋亡率极显著下调(P<0.01)。本研究结果表明:正常睾丸组织结构完整,隐睾间质疏松,且HIGD1C在牦牛隐睾组织中高表达。体外细胞试验表明上调HIGD1C,可以激活促凋亡蛋白,促进牦牛支持细胞凋亡;反之,下调HIGD1C表达水平会抑制支持细胞凋亡。提示HIGD1C参与调控牦牛睾丸支持细胞的凋亡,为揭示牦牛隐睾的发生机制提供了参考。

Research Progress of Nickel Iron Bimetallic Series Electrocatalytic Materials

Hydrogen energy has become one of the recognized clean energy sources worldwide due to its advantages such as low cost,renewable energy,and green environmental protec-tion.Electrolytic water is currently one of the most promising solutions for providing hydrogen fuel.Nickel iron bimetallic electrocatalysts have abundant sources,low cost,clean and pollution-free properties,and strong catalytic performance,This article mainly reviews the development and research of bimetallic nickel iron oxides and nickel iron alloys in recent years,and explores their synthesis methods,properties,and stability in depth.

泌尿系术后发生真菌感染的特点及危险因素分析

目的探讨泌尿系术后发生真菌感染的特点及危险因素,为临床提供依据。方法回顾性分析2017年6月—2021年5月在我院接受泌尿系统手术治疗的762例泌尿系统疾病患者的临床资料,根据其术后是否发生泌尿系、血液真菌感染,分为感染组(n=75)及非感染组(n=687)。比较两组患者临床资料,并进行单因素分析与Logistic回归分析。结果762例患者中75例发生术后真菌感染,发生率为9.84%,其中患者疾病类型以泌尿系结石为主,病原菌以念珠菌为主,导管留置以DJ管、导尿管为主;在75例泌尿系术后发生真菌感染中,69例无症状,5例尿频、尿急、尿痛,1例畏寒、发热、腰痛。单因素分析结果显示,感染组年龄>65岁、开放手术、手术时间≥30 min、引流管留置时间>7 d、抗菌药物使用时间≥3 d所占比例较非感染组更高(P<0.05),血糖水平、CRP水平高于非感染组(P<0.05);Logistic多因素分析结果显示,年龄(OR=1.604)、手术方式(OR=1.139)、导管留置时间(OR=2.537)、血糖水平(OR=2.753)、抗菌药物使用时间(OR=2.508)、CRP(OR=3.057)是影响泌尿系术后发生真菌感染的独立危险因素(P<0.05)。结论泌尿系术后真菌感染的常见病原菌为念珠菌且多无临床症状,常见疾病为泌尿系结石,常见导管留置为DJ管、导尿管。年龄>65岁、开放手术、导管留置时间>7 d、血糖水平升高、抗菌药物使用超过3 d、CRP升高是影响术后泌尿系发生真菌感染的独立危险因素,需进行针对性干预。

Exercise sustains the hallmarks of health

Exercise has long been known for its active role in improving physical fitness and sustaining health.Regular moderate-intensity exercise improves all aspects of human health and is widely accepted as a preventative and therapeutic strategy for various diseases.It is well-documented that exercise maintains and restores homeostasis at the organismal,tissue,cellular,and molecular levels to stimulate positive physiological adaptations that consequently protect against various pathological conditions.Here we mainly summarize how moderate-intensity exercise affects the major hallmarks of health,including the integrity of barriers,containment of local perturbations,recycling and turnover,integration of circuitries,rhythmic oscillations,homeostatic resilience,hormetic regulation,as well as repair and regeneration.Furthermore,we summarize the current understanding of the mechanisms responsible for beneficial adaptations in response to exercise.This review aimed at providing a comprehensive summary of the vital biological mechanisms through which moderate-intensity exercise maintains health and opens a window for its application in other health interventions.We hope that continuing investigation in this field will further increase our understanding of the processes involved in the positive role of moderate-intensity exercise and thus get us closer to the identification of new therapeutics that improve quality of life.

Effect of palmitoylethanolamide on degeneration of a human-derived retinal pigment epithelial cell induced by all-trans retinal

AIM:To study the effect of palmitoylethanolamide(PEA)on apoptosis of retinal pigment epithelial(RPE)cells induced by all-trans retinal(at RAL)and to explore the possible molecular mechanism.METHODS:Cell Titer 96■Aqueous One Solution Cell Proliferation Assay(MTS)was used to detect the effect of PEA on human-derived retinal epithelial cells(ARPE-19)viability induced by at RAL.A Leica DMi8 inverted microscope was used to observe cell morphology.Reactive oxygen species(ROS)production was evaluated with 2’,7’-dichlorodihydrofluorescein diacetate(H2DCFDA)staining and fluorescence microscopy.Expression of c-Jun N-terminal kinase(JNK),phosphorylated JNK(p-JNK),c-Jun,phosphorylated c-Jun(p-c-Jun),Bak,cleaved caspase-3,C/EBP homologous protein(CHOP),and binding(Bip)protein levels were tested by Western blot.Abca4-/-Rdh8-/-mice,mouse models of at RAL clearance defects which displays some symbolic characteristics of dry age-related macular degeneration(AMD)and Stargardt disease(STGD1).In the animal models,PEA was injected intraperitoneally.The full-field electroretinogram was used to detect visual function under scotopic conditions traced from mice.Optical coherence tomography showed reconstitution or thickening of the retinal pigment epithelium layer.Effect of PEA on fundus injury induced by light in Abca4-/-Rdh8-/-mice was observed by fundus photography.RESULTS:PEA ameliorated ARPE-19 cells apoptosis and inhibited ROS(including mitochondrial ROS)production induced by at RAL.PEA improved the retinal functional,prohibited both RPE and photoreceptor from death,ameliorates light-induced fundus impairment in Abca4-/-Rdh8-/-mice.In vitro and in vivo,PEA inhibited JNK,p-JNK,c-Jun,p-c-Jun,Bak,cleaved caspase-3,CHOP,and Bip protein levels induced by all-trans retinal in ARPE-19 cells.CONCLUSION:PEA has effect on treating RPE cells apoptosis in retinopathy caused by at RAL accumulation.PEA is a potential treatment strategy for dry AMD and STGD1.The molecular mechanism is affecting the ROS-JNKCHOP signaling pathway partly.

Origin of a giant fuzzy reflection zone and its implication for natural gas exploration in the southwestern Qiongdongnan Basin of the South China Sea

The southwestern depression of the Qiongdongnan Basin(QDNB)hosts thick Cenozoic sediments and awaits major hydrocarbon discovery.Multichannel seismic(MCS)profile CFT2011 across the southwestern QDNB reveals a~60-km-wide fuzzy reflection zone(FRZ)within the sediments,but its origin and distribution remain unclear.Here ocean bottom seismometer(OBS)data of Line CFT2011 are processed with focus on the velocity structures by traveltime tomography inversion and analyzed together with the coincident and adjacent MCS profiles.The OBS velocity results show that the giant FRZ features lower velocity with difference up to 1.5 km/s and smaller vertical velocity gradient than the surrounding sedimentary sequences at the same depth,likely resulting from enhanced fluid infilling.The MCS profile exhibits that the giant FRZ is about 3-9-km thick and extends from the Paleogene strata rich in organic matters upward to the lower Pleistocene sediments.Within the shallow overlying sediments,multiple bright spots with reverse polarity are imaged and their reflection amplitudes increase with offset,consistent with the features of gas-charged sediments.They are probably shallow gas reservoirs with gases sourced from the deep FRZ.Therefore,the FRZ is proposed to be a giant gas-charged zone,which probably contains lots of hydrocarbon gases migrated vertically from the deep Paleogene source rocks through the boundary faults of the depressions and the minor fractures generated under overpressure.This FRZ is also imaged on the adjacent MCS profiles MCS-L1 and MCS-L2 with the width of about 40 km and 68 km,respectively.It is roughly estimated to cover an area of~1900 km2 and host a volume of~11400 km3 assuming an average thickness of 6 km,implying huge natural gas potential in the sedimentary depression of the southwestern QDNB of the South China Sea.

Anisodine hydrobromide alleviates oxidative stress caused by hypoxia/reoxygenation in human cerebral microvascular endothelial cells predominantly via inhibition of muscarinic acetylcholine receptor 4

Background:Anisodine hydrobromide(AT3),an anti-cholinergic agent,could be delivered to the brain across the blood-brain barrier and has been used clinically for the treatment of cerebral ischemia/reperfusion injury.Endothelial dysfunction can be caused by hypoxia/reoxygenation(H/R)via oxidative stress and metabolic alterations.The present study investigated whether AT3 regulates the production of nitric oxide(NO)and reactive oxygen species(ROS),and the HIF-1αpathway via regulation of muscarinic acetylcholine receptors(mAChRs)in brain microvascular endothelial cells after H/R exposure.Methods:Under H/R conditions,hCMEC/D3 cerebral microvascular endothelial cells were treated with AT3.Specific inhibitors of M2-and M4-mAChRs were used to explore the mechanism by which AT3 influences oxidative stress in endothelial cells.Then,mAChRs expression was detected by western blotting and NO production was detected by Greiss reaction.The intracellular ROS level was measured using DCFH-DA probes.The expression of hypoxia-inducible transcription factor 1α(HIF-1α)was also detected.Results:While H/R induced the expression of M2-and M4-mAChRs,AT3 suppressed the H/R-upregulated M2-and M4-mAChRs.H/R also induced the production of NO,ROS,and apoptosis.AT3 and M4-mAChR inhibitors inhibited the H/R-induced production of NO and ROS and apoptosis.HIF-1αwas induced by H/R,but was suppressed by AT3.Conclusion:Thus,the in vitro evidence shows that AT3 protects against H/R injury in cerebral microvascular endothelial cells via inhibition of HIF-1α,NO and ROS,predominantly through the downregulation of M4-mAChR.The findings offer novel understandings regarding AT3-mediated attenuation of endothelial cell apoptosis and cerebral ischemia/reperfusion injury.

多媒体健康教育下的量化踝泵运动在预防肝硬化患者股静脉置管深静脉血栓形成中的应用

目的 探讨多媒体健康教育下的量化踝泵运动在预防肝硬化患者股静脉置管深静脉血栓形成中的应用价值.方法 选取我院2014年12月至2017年10月接受股静脉置管的肝硬化患者64例,随机数字表法分为对照组(n=32)与研究组(n=32).对照组给予常规护理,研究组于常规护理基础上采取多媒体健康教育下量化踝泵运动干预.统计两组股静脉置管后第7d、14d下肢深静脉血栓发生率及护理满意度.结果 研究组股静脉置管后第7d、14d下肢深静脉血栓发生率(0.00%、3.13%)低于对照组(6.25%、12.50%) (P<0.05);研究组护理满意度(93.75%)高于对照组(71.88%)(P<0.05).结论 肝硬化患者股静脉置管期间实施多媒体健康教育下量化踝泵运动,可有效降低深静脉血栓发生风险,且利于提高患者护理满意度.

不造人工肾积水经皮肾镜碎石术临床效果观察

目的探讨不造人工肾积水经皮肾镜碎石术的临床效果。方法回顾性分析2016年6月-2021年6月在四川省宣汉县人民医院接受了经皮肾镜碎石术的患者(术前肾积水程度:10~25 mm)资料,根据手术方式将其分为人工肾积水手术(AH)组和非人工肾积水手术(NAH)组,对比分析两组患者的一般资料、术前指标、术中情况、术后围手术期指标及医疗费用。结果共166例患者被纳入研究,AH组98例、NAH组68例,NAH组手术时间较AH组短[(87.8±26.6)min vs.(104.2±23.0)min,P=0.001],手术总费用低[(15617±3502)元vs.(16866±3490)元,P=0.025],术后发热发生率低(4.4%vs.16.3%,P=0.018),两组间其余临床资料差异无统计学意义。结论轻度肾积水患者可在不造人工肾积水的情况下进行经皮肾镜碎石术,该方法能缩短手术时间、降低发热风险、减少手术费用。

最大流灵敏度分析算法及在交通管理中的应用

由于道路突发事件或地铁施工,会使事件所在道路通行能力下降,使路网运输能力受到影响。这种情况下,交管部门一般会采用一些交通管制措施,将事件道路上的交通流引导到其他路段上,以减轻对堵塞路段的压力。论文提出路网局部能力下降时,网络流关于容量的灵敏度分析算法,获得新的最小费用最大流分配方案,为交通组织方案提供依据。

中药单体抗皮肤鳞状细胞癌研究进展

皮肤鳞状细胞癌是一种发病率较高的恶性肿瘤,病情发展快,破坏性大,可发生多处转移,严重威胁着人类的生命健康。在临床治疗肿瘤过程中,手术、放化疗等基本手段显现出明显的不良反应和局限性,近年来中药以其安全性高、不良反应小等抗肿瘤作用日益受到医学界重视。随着对中药研究的深入,从中药中提取分离的有效单体在生物活性方面表现出多种抗肿瘤作用。该篇综述总结了当前中药单体对皮肤鳞状细胞癌的研究进展,旨在为临床应用和深入研究提供参考。

UKF地铁短时客流预测研究

为获得较为可靠的地铁车站实时客流,提出基于神经网络与无迹变换卡尔曼滤波(UKF)结合的信息融合预测方法。首先利用各站点间进出客流时空相关性,在运行时间约束下组织预测向量,以BP神经网络为函数表达给出目标站点客流的初步预测值。在此基础上,利用无迹变换卡尔曼滤波解决神经网络过学习造成的误差,以提高预测结果精度。最后选取实例验证算法的准确性,结果表明,该改进算法可有效提高预测精度,满足运营需求。

以课程国际化建设为核心的来华留学生MBBS教育

随着我国教育国际化进程的不断推进,临床医学专业(MBBS)留学生与日俱增,如何解决课程教育国际化建设,提高教学质量成为医学院校面临的重要课题。大连医科大学MBBS人才培养从传统的单纯传授医学知识转变为搭建知识构架、培养专业能力、提高综合素质,并以美国知名医学院校的教学模式和课程体系为参考,通过实施学分制管理,设置课程模块和课程组,融合教学内容和知识点,开展以问题导向的教学,临床病例实践教学、引入与国际接轨的规范化考核评价体等促进来华留学生MBBS教育与国际接轨,提高留学生分析和解决问题能力、临床思维能力和主动学习能力,全面提高了学校MBBS专业教育质量。

广西北部湾地区猪圆环病毒2型检测及其ORF2遗传变异分析

为了解猪圆环病毒2型(Porcine circovirus type 2,PCV2)在广西北部湾地区的流行情况和其ORF2基因的遗传变异规律,本研究采用PCR方法对2016年采自广西北部湾地区规模猪场和农村散养户的306份疑似PCV2感染猪的血液和组织样品进行了PCV2的病原学检测,同时对分离的4株PCV2 ORF2基因进行PCR扩增、克隆和测序,并与Gen Bank中登录的34株国内外PCV2参考株ORF2序列进行比对和遗传进化分析。结果表明:PCV2检测阳性样品为129份,阳性率为42.16%。随机选取的4份阳性病料PCV2 ORF2基因测序结果显示,4个毒株遗传关系上均属于Group 1(PCV2b),其中BBW-1和BBW-2分离株属于1A分支且分别与Fd3株(AY321984)和NL_PMWS_3株(AY484415)同源性较高,BBW-3和BBW-4分离株属于1B分支且BBW-4分离株与Hu Zhou0301株(AY536756)和NL_Control_1株(AY484407)同源性较高,其ORF2基因核苷酸和氨基酸同源性分别为98.9%~99.5%和93.9%~95.4%,说明该地区PCV2的优势基因型是PCV2b。研究结果不仅有利于掌握广西北部湾地区PCV2流行毒株同源性情况,而且将为该地区(porcine circovirus associated disease,PCVAD)的防治和新型疫苗的研制提供依据。

miR-223-3p靶向调控Rbpj转录因子对葡萄膜炎大鼠Th1和Th17细胞分化的影响

目的:探讨miR-223-3p对Notch信号通路转录因子Rbpj表达的调控作用以及对实验性自身免疫性葡萄膜炎(EAU)大鼠Th1、Th17细胞分化水平的影响。方法:用双荧光素酶表达报告系统探讨miR-223-3p对Rbpj基因表达的调控作用。将24只雌性Lewis大鼠随机分为EAU模型组、正常对照组(NC组)和空白对照组(BC组),每组8只。EAU模型组注射含光感受器间维生素A结合蛋白(IRBP)、结核菌素和完全弗氏佐剂的乳糜液以诱导葡萄膜炎,BC组注射等体积的不含IRBP多肽的乳糜液,NC组注射等体积的无菌生理盐水。免疫后12d,无菌分离三组大鼠的脾脏、淋巴结和眼组织,实时荧光定量PCR(Q-PCR)检测miR-223-3p和Rbpj基因的表达水平;酶联免疫吸附测定(ELISA)方法检测Rbpj、IFN-γ、IL-17蛋白的表达水平;流式细胞仪检测三组大鼠不同组织中Th1和Th17细胞的表达水平。结果:双荧光素酶检测结果证实Rbpj是miR-223-3p调控的靶基因。免疫后12d,相比于NC组,EAU模型组大鼠脾脏、淋巴结和眼组织中miR-223-3p相对表达水平分别为0.33±0.29、0.11±0.12、0.18±0.11,均呈下调表达(均P<0.05);Rbpj mRNA水平均呈上调表达,分别为3.00±0.06、1.52±0.12、3.01±0.34(均P<0.05);BC组大鼠脾脏、淋巴结和眼组织中miR-223-3p和Rbpj mRNA水平与NC组相比均无差异(P>0.05)。ELISA检测结果发现,免疫后12d的EAU模型组大鼠各组织中Rbpj、IFN-γ、IL-17蛋白表达水平均明显高于NC组(均P<0.05),BC组大鼠各组织中Rbpj、IFN-γ、IL-17蛋白表达水平与NC组相比均无差异(P>0.05)。流式细胞仪检测结果显示,免疫后12d,EAU模型组各组织的Th1和Th17细胞比例均显著高于NC组(均P<0.05)。BC组与NC组各组织Th1和Th17细胞比例无明显变化(均P>0.05)。结论:miR-223-3p可负调控Notch信号通路转录因子Rbpj的表达。EAU大鼠中miR-223-3p的下调表达可升高Rbpj基因和蛋白的表达水平,促进Th1和Th17细胞的分化以及提高相关分子IFN-γ和IL-17的表达水平,进而影响葡萄膜炎的发生发展。

微量元素硒在动物体内的抗病毒作用研究进展

人体正常生命活动离不开微量元素硒,硒具有良好的抗氧化性、抗肿瘤性和抗重金属性,不仅能参与调节机体免疫反应和激素水平,而且可以抑制多种病毒的复制。人体对硒摄取过多或不足都会导致相应疾病。由于自然界硒分布不均,我国人口普遍缺硒,适当且有效地补硒对打造全民健康极其重要。本文以近年来国内外最新研究资料为基础,对硒的几种典型抗病毒作用进行了系统的总结,以期为硒抗病毒作用相关研究提供理论参考,为富硒食品、含硒药物、富硒保健品等硒产品的研发与推广提供理论支撑。

Upregulation of caveolin-1 and SR-B1 in mice with non-alcoholic fatty liver disease

BACKGROUND:Non-alcoholic fatty liver disease(NAFLD)is one of the most frequent causes of liver diseases,with markedly increased prevalence.However,its mechanisms are not clear.The present study was undertaken to illustrate the role of caveolin-1(cav1)and the scavenger receptor class B type 1(SR-B1)in NAFLD.METHODS:Adult male C57BL/6 mice were fed with a normal diet or high fat and cholesterol(HFC)diet for 14 weeks.The mice were sacrificed to collect plasma and harvest the liver;their plasma lipid concentration was measured.Hepatic cav1and SR-B1 mRNA and protein expression were determined by real-time quantitative polymerase chain reaction(qPCR)and Western blotting,respectively.In order to study cav1 and SR-B1distribution and change in hepatocytes,immunohistochemical analysis was performed.RESULTS:HFC diet increased plasma lipids,induced NAFLD and increased the liver/body weight ratio.Compared to the control mice(n=6),the mRNA and protein levels of cav1 and SR-B1 in liver tissue of the NAFLD mice(n=12)increased significantly(cav1 mRNA:1.536±0.226 vs 0.980±0.272,P【0.05;protein:0.643±0.240 vs 0.100±0.130,P【0.01;SR-B1 mRNA:1.377±0.125 vs 0.956±0.151,P【0.01;protein:2.156±0.507vs 0.211±0.211,P【0.01).Furthermore,both cav1 and SR-B1immunoreactivity increased and their distribution was also changed,mainly in the plasma membrane of hepatocytes,cytoplasm and membrane of lipid droplets and around.CONCLUSION:NAFLD is associated with increased concentration of plasma lipids and upregulation of hepatic cav1 and SR-B1 gene and protein expressions,which indicate that cav1 and SR-B1 might play crucial roles in the pathogenesis of NAFLD.

基于Cap蛋白研制的猪圆环病毒2型疫苗研究进展

猪圆环病毒相关疾病(PCVAD)是危害世界养猪业的主要疫病之一,主要由猪圆环病毒2型(PCV-2)引起。该病毒可引起感染猪免疫系统的破坏,造成严重的免疫抑制,继而引发多种病毒/细菌的混合感染与继发感染,给世界养猪业造成了巨大经济损失。近年来疫苗免疫接种是防控PCVAD的有效手段,由于衣壳蛋白(capside,Cap)是PCV-2的主要免疫保护性抗原,因此常被用作研制PCV-2基因工程疫苗的理想靶抗原。论文就PCV-2Cap蛋白基因工程疫苗的研究进展进行综述,以期为今后PCV-2新型疫苗的研究提供参考。

A New Alkaloid from Patrinia scabra

A new alkaloid has been isolated from the root of Patrinia scabra. Its structure was elucidated as 2'-acetamido-3'-phenyl propyl 2-benzamido-3-phenyl propionate by extensive spectroscopic analysis.

阿奇霉素两种给药方案治疗小儿支原体肺炎的疗效及经济学评价

目的:探讨阿奇霉素两种给药方案治疗小儿支原体肺炎的疗效及经济学评价。方法:选取2017年3月—2017年11月解放军第九七医院收治的支原体肺炎患儿120例,进行回顾性分析,根据用药方案将其分为序贯治疗组和持续静脉滴注组,每组60例。持续静脉滴注组患儿给予阿奇霉素10 mg/kg,静脉滴注,1日1次,连用3 d、停用4 d为1个疗程,治疗2个疗程。序贯治疗组患儿给予阿奇霉素10 mg/kg,静脉滴注,1日1次,连用3 d,停用4 d后口服阿奇霉素10 mg/kg,1日1次,连用3 d。比较两组患儿症状、体征、X线检查变化情况、临床疗效、不良反应发生情况及经济学评价的差异。结果:两组患儿退热时间、咳嗽消失、肺部啰音消失时间及X线检查恢复正常时间的差异均无统计学意义(P>0.05);持续静脉滴注组、序贯治疗组患儿的总有效率分别为90.00%(54/60)、91.67%(55/60),差异无统计学意义(P>0.05);序贯治疗组患儿不良反应发生率为5.00%(3/60),明显低于持续静脉滴注组的11.67%(7/60),差异有统计学意义(P<0.05);持续静脉滴注组方案的成本-效果比为5.55,序贯治疗组为2.88。结论:与持续静脉滴注相比,阿奇霉素序贯治疗法用于小儿支原体肺炎的成本-效果较好。