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化学实验安全课程思政的教学设计与探索——以黑龙江大学“化学实验安全”课程为例 被引量:1
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作者 秦川丽 范乃英 +6 位作者 王岩 王彬 张国 郑冰 屈宜春 孙治尧 安光辉 《大学化学》 CAS 2024年第2期234-241,共8页
分析了当前高校实验室安全教育课程思政存在的问题,阐述了“课程思政”在实验室安全教育中的育人意义,结合实验室安全教育课程的基本特征,总结了课程围绕教学目标、教学内容、教学方法、教学评价、师资队伍五个层面进行的课程思政教学... 分析了当前高校实验室安全教育课程思政存在的问题,阐述了“课程思政”在实验室安全教育中的育人意义,结合实验室安全教育课程的基本特征,总结了课程围绕教学目标、教学内容、教学方法、教学评价、师资队伍五个层面进行的课程思政教学设计与探索。构建的课程思政教学体系在培养学生化学实验安全知识和技能的同时,提升了学生的安全意识、家国情怀、品德修为、科学精神,助力于培养德才兼备的新时代高素质化学人才。 展开更多
关键词 化学实验安全 课程思政 教学设计
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共表达IL-7/CCL19的新抗原反应性T细胞对小鼠肺癌的抗肿瘤研究
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作者 吴迪 李晨辉 +5 位作者 汪艳 何正强 金常娥 郭敏 陈荣昌 周承志 《中国肺癌杂志》 CAS CSCD 北大核心 2024年第7期504-513,共10页
背景与目的新抗原反应性T细胞(neoantigen reactive T cell,NRT)具有抑制表达特异性新抗原的肿瘤生长的能力。然而,由于免疫浸润困难和肿瘤微环境的抑制,NRT在实体瘤中的治疗效果有限。本研究针对小鼠肺癌细胞设计出可以同时表达白细胞... 背景与目的新抗原反应性T细胞(neoantigen reactive T cell,NRT)具有抑制表达特异性新抗原的肿瘤生长的能力。然而,由于免疫浸润困难和肿瘤微环境的抑制,NRT在实体瘤中的治疗效果有限。本研究针对小鼠肺癌细胞设计出可以同时表达白细胞介素7(interleukin 7,IL-7)和趋化因子19(chemokine C-C motif ligand 19,CCL19)的NRT细胞(7×19 NRT),并对7×19 NRT细胞和常规NRT细胞的抗肿瘤效果差异进行了评估。方法针对小鼠Lewis肺癌细胞(Lewis lung carcinoma,LLC)进行了新一代测序和新抗原预测,制备了RNA疫苗,培养了NRT细胞,构建了编码IL-7和CCL19的逆转录病毒载体,转导NRT细胞并成功表达IL-7和CCL19,成功获得了7×19 NRT,并在小鼠体内外对其抗肿瘤效果进行了评估。结果7×19 NRT细胞通过分泌IL-7和CCL19显著增强T细胞的增殖和侵袭能力,在小鼠肺癌中实现了显著的抑瘤作用,延长了小鼠的生存期。经7×19 NRT治疗后,T细胞浸润肿瘤组织及肿瘤组织坏死显著增加。此外,7×19 NRT治疗与常规NRT治疗均安全。结论通过IL-7和CCL19的表达,NRT细胞的抗实体瘤能力显著增强,这是一种对NRT安全有效的基因修饰。 展开更多
关键词 新抗原 白细胞介素7 趋化因子19 肺肿瘤 新抗原反应性T细胞
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化学实验安全课程思政教学案例——以实验室常见仪器设备及玻璃装置安全使用为例
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作者 范乃英 秦川丽 +6 位作者 张国 王彬 王岩 郑冰 屈宜春 孙治尧 安光辉 《大学化学》 CAS 2024年第2期242-247,共6页
实验室常见仪器设备及玻璃装置的安全使用是实验室必备的知识和技能。本案例将专业知识与思政元素有机融合,将实验室常见仪器设备及玻璃装置进行安全实验与实验室安全事故及实际生活相联系,围绕学生核心素养为导向的多融合教学设计与实... 实验室常见仪器设备及玻璃装置的安全使用是实验室必备的知识和技能。本案例将专业知识与思政元素有机融合,将实验室常见仪器设备及玻璃装置进行安全实验与实验室安全事故及实际生活相联系,围绕学生核心素养为导向的多融合教学设计与实施,进一步强化了学生的安全与环保意识,激发了学生的学习兴趣,并对MOOC中相应知识进行了延伸、拓展和补充,培养了学生的知识迁移能力,让学生充分认识到实验室仪器设备及玻璃装置安全使用对保障实验安全以及国家安全稳定的重要意义。增强了学生的社会责任感,对提升学生的能力和素质发挥了良好作用。 展开更多
关键词 化学实验安全 课程思政 案例设计
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烧结温度对石墨烯填充聚醚醚酮/聚四氟乙烯摩擦磨损性能的影响
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作者 刘林 汤赫男 +1 位作者 王妍 赵晶 《高分子材料科学与工程》 EI CAS CSCD 北大核心 2024年第3期98-109,共12页
为探究石墨烯填充聚醚醚酮/聚四氟乙烯(PEEK/PTFE)复合材料制备过程中烧结温度对复合材料摩擦磨损性能的影响,基于分子动力学模拟研究方法,分别构建10%PEEK/PTFE和1%石墨烯/10%PEEK/PTFE体系模型,通过模拟复合材料制备过程中不同的烧结... 为探究石墨烯填充聚醚醚酮/聚四氟乙烯(PEEK/PTFE)复合材料制备过程中烧结温度对复合材料摩擦磨损性能的影响,基于分子动力学模拟研究方法,分别构建10%PEEK/PTFE和1%石墨烯/10%PEEK/PTFE体系模型,通过模拟复合材料制备过程中不同的烧结温度,得到复合材料对应的力学和摩擦学性能相关参数。在不同烧结温度制得10%PEEK/PTFE和1%石墨烯/10%PEEK/PTFE样品,进行了摩擦磨损试验及磨损形貌分析。结果表明,采用石墨烯填充PEEK/PTFE复合材料可以有效改善复合材料的摩擦磨损特性,不同烧结温度对复合材料分子间作用力、材料力学特性和摩擦特性表现出不同程度的影响,当烧结温度为360℃时,复合材料分子间作用力、材料力学特性和摩擦特性均达到峰值。 展开更多
关键词 烧结温度 分子动力学模拟 聚四氟乙烯 聚醚醚酮 石墨烯
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氟硅协同增强的UV固化透明高硬度双疏涂层的制备及性能
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作者 孙侠 张智慧 +4 位作者 孙小英 刘晶 王艳 杭建忠 施利毅 《高分子材料科学与工程》 EI CAS CSCD 北大核心 2024年第6期125-132,共8页
以全氟辛基乙基丙烯酸酯(17F)和四(3-巯基丙酸)季戊四醇酯(PETMP)为原料,通过迈克尔加成反应合成了氟改性多巯基交联剂(FPETMP)。红外光谱和核磁共振氢谱表征证实17F成功改性了多巯基交联剂,且合成的FPETMP中仍有未反应的巯基。将FPETMP... 以全氟辛基乙基丙烯酸酯(17F)和四(3-巯基丙酸)季戊四醇酯(PETMP)为原料,通过迈克尔加成反应合成了氟改性多巯基交联剂(FPETMP)。红外光谱和核磁共振氢谱表征证实17F成功改性了多巯基交联剂,且合成的FPETMP中仍有未反应的巯基。将FPETMP与PETMP、(丙烯酰氧基丙基)甲基硅氧烷均聚物(APMS)混合,经紫外光引发巯基-烯点击反应制得透明双疏涂层。研究了FPETMP用量对涂层疏水疏油性、透明性和力学性能的影响。结果表明,随着FPETMP含量的增加,涂层的疏水疏油性增强。但是FPETMP用量太高时,体系相容性下降,涂层的透明度和铅笔硬度也随之变差。当FPETMP的质量分数为20%时,涂层对水和十六烷的接触角分别为116.5°和75.6°,且涂层的硬度较高,铅笔硬度为7H。涂层呈现出良好的光学透明性,综合性能最佳。 展开更多
关键词 UV固化 双疏涂层 光学透明 硬度
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Melatonin improves synapse development by PI3K/Akt signaling in a mouse model of autism spectrum disorder 被引量:2
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作者 Luyi wang Man Xu +8 位作者 yan wang Feifei wang Jing Deng Xiaoya wang Yu Zhao Ailing Liao Feng yang Shali wang Yingbo Li 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第7期1618-1624,共7页
Autism spectrum disorders are a group of neurodevelopmental disorders involving more than 1100 genes,including Ctnnd2 as a candidate gene.Ctnnd2knockout mice,serving as an animal model of autis m,have been demonstrate... Autism spectrum disorders are a group of neurodevelopmental disorders involving more than 1100 genes,including Ctnnd2 as a candidate gene.Ctnnd2knockout mice,serving as an animal model of autis m,have been demonstrated to exhibit decreased density of dendritic spines.The role of melatonin,as a neuro hormone capable of effectively alleviating social interaction deficits and regulating the development of dendritic spines,in Ctnnd2 deletion-induced nerve injury remains unclea r.In the present study,we discove red that the deletion of exon 2 of the Ctnnd2 gene was linked to social interaction deficits,spine loss,impaired inhibitory neurons,and suppressed phosphatidylinositol-3-kinase(PI3K)/protein kinase B(Akt) signal pathway in the prefrontal cortex.Our findings demonstrated that the long-term oral administration of melatonin for 28 days effectively alleviated the aforementioned abnormalities in Ctnnd2 gene-knockout mice.Furthermore,the administration of melatonin in the prefro ntal cortex was found to improve synaptic function and activate the PI3K/Akt signal pathway in this region.The pharmacological blockade of the PI3K/Akt signal pathway with a PI3K/Akt inhibitor,wo rtmannin,and melatonin receptor antagonists,luzindole and 4-phenyl-2-propionamidotetralin,prevented the melatonin-induced enhancement of GABAergic synaptic function.These findings suggest that melatonin treatment can ameliorate GABAe rgic synaptic function by activating the PI3K/Akt signal pathway,which may contribute to the improvement of dendritic spine abnormalities in autism spectrum disorders. 展开更多
关键词 AUTISM Ctnnd2 deletion GABAergic neurons MELATONIN PI3K/Akt signal pathway prefrontal cortex social behavior spine density synaptic-associated proteins
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基于斜塔芳烃的晶体工程研究
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作者 吴佳睿 吴庚辛 +1 位作者 王艳 杨英威 《大学化学》 CAS 2024年第3期58-62,共5页
凭借固有的分子识别和络合能力,大环主体分子一直是超分子化学研究所依赖的重要工具,同时也大力地推动了现代超分子化学的蓬勃发展并从基础研究走向实际应用。斜塔芳烃做为一类新型芳烃大环受体具有易溶解、易结晶、良好的骨架柔性和主... 凭借固有的分子识别和络合能力,大环主体分子一直是超分子化学研究所依赖的重要工具,同时也大力地推动了现代超分子化学的蓬勃发展并从基础研究走向实际应用。斜塔芳烃做为一类新型芳烃大环受体具有易溶解、易结晶、良好的骨架柔性和主客体化学性质等特点和优势,自其诞生以来便在晶体工程和相关研究领域得到广泛应用和发展。本文将从晶体工程角度出发来介绍若干有关斜塔芳烃的应用研究实例,重点突出结构与功能之间的内在关联,以期提高学生对晶体化学及其交叉学科的学习兴趣和内在驱动力。 展开更多
关键词 晶体工程 超分子化学 大环化学 主客体化学
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Enhancing the stability of Ni Fe-layered double hydroxide nanosheet array for alkaline seawater oxidation by Ce doping 被引量:1
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作者 Yongchao Yao Shengjun Sun +14 位作者 Hui Zhang Zixiao Li Chaoxin yang Zhengwei Cai Xun He Kai Dong Yonglan Luo yan wang Yuchun Ren Qian Liu Dongdong Zheng Weihua Zhuang Bo Tang Xuping Sun Wenchuang(Walter)Hu 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第4期306-312,共7页
Electrocatalytic hydrogen production from seawater holds enormous promise for clean energy generation.Nevertheless,the direct electrolysis of seawater encounters significant challenges due to poor anodic stability cau... Electrocatalytic hydrogen production from seawater holds enormous promise for clean energy generation.Nevertheless,the direct electrolysis of seawater encounters significant challenges due to poor anodic stability caused by detrimental chlorine chemistry.Herein,we present our recent discovery that the incorporation of Ce into Ni Fe layered double hydroxide nanosheet array on Ni foam(Ce-Ni Fe LDH/NF)emerges as a robust electrocatalyst for seawater oxidation.During the seawater oxidation process,CeO_(2)is generated,effectively repelling Cl^(-)and inhibiting the formation of Cl O-,resulting in a notable enhancement in the oxidation activity and stability of alkaline seawater.The prepared Ce-Ni Fe LDH/NF requires only overpotential of 390 m V to achieve the current density of 1 A cm^(-2),while maintaining long-term stability for 500 h,outperforming the performance of Ni Fe LDH/NF(430 m V,150 h)by a significant margin.This study highlights the effectiveness of a Ce-doping strategy in augmenting the activity and stability of materials based on Ni Fe LDH in seawater electrolysis for oxygen evolution. 展开更多
关键词 Ce doping NiFe layered double hydroxide Seawater oxidation Electrocatalysis Cl^(-) repulsion
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Mechanistic investigation on Ag-Cu_(2)O in electrocatalytic CO_(2) to CH_(4) by in situ/operando spectroscopic and theoretical analysis 被引量:1
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作者 Min Sun Luxiao Zhang +10 位作者 Fuli Tian Jiaxin Li yanqiu Lei Heng Zhang Lifeng Han Zhihua Guo Yonghui Gao Fenrong Liu yan wang Luhui wang Shanghong Zeng 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第1期521-531,I0012,共12页
Silver-copper electrocatalysts have demonstrated effectively catalytic performance in electroreduction CO_(2) toward CH_(4),yet a revealing insight into the reaction pathway and mechanism has remained elusive.Herein,w... Silver-copper electrocatalysts have demonstrated effectively catalytic performance in electroreduction CO_(2) toward CH_(4),yet a revealing insight into the reaction pathway and mechanism has remained elusive.Herein,we construct chemically bonded Ag-Cu_(2)O boundaries,in which the complete reduction of Cu_(2)O to Cu has been strongly impeded owing to the presence of surface Ag shell.The interfacial confinement effect helps to maintain Cu^(+)sites at the Ag-Cu_(2)O boundaries.Using in situ/operando spectroscopy and theoretical simulations,it is revealed that CO_(2) is enriched at the Ag-Cu_(2)O boundaries due to the enhanced physisorption and chemisorption to CO_(2),activating CO_(2) to form the stable intermediate^(*)CO.The boundaries between Ag shell and the Cu_(2)O mediate local^(*)CO coverage and promote^(*)CHO intermediate formation,consequently facilitating CO_(2)-to-CH_(4) conversion.This work not only reveals the structure-activity relationships but also offers insights into the reaction mechanism on Ag-Cu catalysts for efficient electrocatalytic CO_(2) reduction. 展开更多
关键词 Ag shell Interfacial confinement effect Cu~+ Local*CO coverage CO_(2)reduction
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High responsivity photodetectors based on graphene/WSe_(2) heterostructure by photogating effect 被引量:1
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作者 李淑萍 雷挺 +5 位作者 严仲兴 王燕 张黎可 涂华垚 时文华 曾中明 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第1期728-733,共6页
Graphene, with its zero-bandgap electronic structure, is a highly promising ultra-broadband light absorbing material.However, the performance of graphene-based photodetectors is limited by weak absorption efficiency a... Graphene, with its zero-bandgap electronic structure, is a highly promising ultra-broadband light absorbing material.However, the performance of graphene-based photodetectors is limited by weak absorption efficiency and rapid recombination of photoexcited carriers, leading to poor photodetection performance. Here, inspired by the photogating effect, we demonstrated a highly sensitive photodetector based on graphene/WSe_(2) vertical heterostructure where the WSe_(2) layer acts as both the light absorption layer and the localized grating layer. The graphene conductive channel is induced to produce more carriers by capacitive coupling. Due to the strong light absorption and high external quantum efficiency of multilayer WSe_(2), as well as the high carrier mobility of graphene, a high photocurrent is generated in the vertical heterostructure. As a result, the photodetector exhibits ultra-high responsivity of 3.85×10~4A/W and external quantum efficiency of 1.3 × 10~7%.This finding demonstrates that photogating structures can effectively enhance the sensitivity of graphene-based photodetectors and may have great potential applications in future optoelectronic devices. 展开更多
关键词 WSe_(2) HETEROSTRUCTURE PHOTODETECTOR photogating effect
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RYR1基因突变和表达异常在胃癌发生发展中作用机制研究
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作者 刘晨冉 程亚楠 +2 位作者 王妍 尉迟之光 于津浦 《中国肿瘤临床》 CAS CSCD 北大核心 2024年第6期271-280,共10页
目的:探讨RYR1基因与胃癌发生发展的相关性以及RYR1促进胃癌恶性进展的作用机制。方法:分析TCGA数据库胃癌患者数据,并回顾性选取2010年12月至2012年12月就诊于天津医科大学肿瘤医院的81例胃癌患者的组织样本进行高通量靶向测序和转录... 目的:探讨RYR1基因与胃癌发生发展的相关性以及RYR1促进胃癌恶性进展的作用机制。方法:分析TCGA数据库胃癌患者数据,并回顾性选取2010年12月至2012年12月就诊于天津医科大学肿瘤医院的81例胃癌患者的组织样本进行高通量靶向测序和转录组测序(TJMUCH队列),收集临床病理信息,比较RYR1基因突变与表达之间相关性,分析RYR1基因对胃癌患者预后的影响,构建RYR1过表达细胞系探索RYR1促进胃癌发生发展的作用机制。结果:在TCGA数据库胃癌患者中,亚洲人群RYR1突变率更高(12.68%vs. 8.13%),在TJMUCH队列胃癌患者中,RYR1基因突变率位于第9位,突变率为33.33%。RYR1突变与表达水平显著负相关(P=0.006 9,P<0.000 1),但RYR1高表达患者的总生存期显著差于低表达RYR1患者(P=0.009 0,P=0.042 0)。RYR1的过表达会促进细胞增殖、迁移、侵袭,减少凋亡,且可下调胃癌细胞对化疗药物的敏感性。抑制RYR1介导的钙离子过度释放可以抑制其恶性生物学行为,并逆转化疗耐药。结论:RYR1基因在亚洲胃癌患者中突变率较高,RYR1突变与表达水平显著负相关,RYR1高表达是胃癌新的预后预测标志物。RYR1过表达可增加内质网钙离子释放而促进胃癌恶性进展,并产生化疗抵抗。靶向阻断RYR1活性可抑制胃癌细胞的增殖、迁移和侵袭,并逆转化疗药抵抗,为胃癌的联合治疗提供新思路。 展开更多
关键词 胃癌 RYR1基因 基因突变 基因表达 钙离子
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Generation of mitochondrial replacement monkeys by female pronucleus transfer 被引量:1
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作者 Chun-yang Li Xing-Chen Liu +6 位作者 Yu-Zhuo Li yan wang yan-Hong Nie Yu-Ting Xu Xiao-Tong Zhang Yong Lu Qiang Sun 《Zoological Research》 SCIE CSCD 2024年第2期292-298,共7页
Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising st... Mutations in mitochondrial DNA(mtDNA)are maternally inherited and have the potential to cause severe disorders.Mitochondrial replacement therapies,including spindle,polar body,and pronuclear transfers,are promising strategies for preventing the hereditary transmission of mtDNA diseases.While pronuclear transfer has been used to generate mitochondrial replacement mouse models and human embryos,its application in non-human primates has not been previously reported.In this study,we successfully generated four healthy cynomolgus monkeys(Macaca fascicularis)via female pronuclear transfer.These individuals all survived for more than two years and exhibited minimal mtDNA carryover(3.8%–6.7%),as well as relatively stable mtDNA heteroplasmy dynamics during development.The successful establishment of this nonhuman primate model highlights the considerable potential of pronuclear transfer in reducing the risk of inherited mtDNA diseases and provides a valuable preclinical research model for advancing mitochondrial replacement therapies in humans. 展开更多
关键词 Non-human primates Mitochondrial replacement Female pronuclear transfer
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氧化苦参碱对小鼠皮肤屏障功能障碍的修复作用研究
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作者 刘兆亿 陈鑫宇 +3 位作者 王艳 李雪 郭若曦 张晗 《日用化学工业(中英文)》 CAS 北大核心 2024年第7期777-783,共7页
探讨氧化苦参碱(OMT)乳膏对小鼠皮肤屏障功能的修复作用。将70只雄性ICR小鼠随机分为7组,除正常组小鼠外,采用胶带粘贴法模拟机械损伤,复制皮肤屏障功能障碍小鼠模型。造模后经皮连续给药5天,拍摄照片并测定造模区域的经表皮失水量(TEWL... 探讨氧化苦参碱(OMT)乳膏对小鼠皮肤屏障功能的修复作用。将70只雄性ICR小鼠随机分为7组,除正常组小鼠外,采用胶带粘贴法模拟机械损伤,复制皮肤屏障功能障碍小鼠模型。造模后经皮连续给药5天,拍摄照片并测定造模区域的经表皮失水量(TEWL)。实验结束后,HE染色观察皮肤组织形态学变化,酶联免疫吸附法(ELISA)检测皮肤组织中肿瘤坏死因子α(TNF-α)、白细胞介素-8(IL-8)的含量,通过实时定量PCR(RT-qPCR)检测紧密连接蛋白(ZO-1)、丝聚蛋白(FLG)、水通道蛋白3(AQP3)mRNA的表达量,通过Western blot检测屏障相关蛋白如封闭蛋白(OCC)、FLG、紧密连接蛋白(Claudin)的表达量。结果显示,与模型组相比,OMT各剂量组均能降低皮肤TEWL,减轻炎症浸润,改善皮肤病理状态,同时高剂组皮肤组织中TNF-α等炎症因子的含量显著降低(P<0.05),ZO-1、FLG m RNA及OCC、FLG、Claudin蛋白的表达显著升高(P<0.05)。因此,OMT具有修复皮肤屏障功能的作用。 展开更多
关键词 氧化苦参碱 皮肤屏障 小鼠
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水通道蛋白1作为急性呼吸窘迫综合征肺部炎症水平的新生物标志物探索研究 被引量:1
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作者 郭星星 张欢欢 +3 位作者 李静 蔡志贵 剡旺 宋立强 《空军军医大学学报》 CAS 2024年第4期418-424,共7页
目的探讨急性呼吸窘迫综合征(ARDS)肺组织水通道蛋白1(AQP1)表达水平的时效规律以及与肺损伤程度、经典炎症指标的相关性,旨在为ARDS寻找新的生物标志物及干预靶点。方法脂多糖(LPS)滴鼻制备ARDS小鼠模型,每日称量小鼠体质量,取6 h、1 d... 目的探讨急性呼吸窘迫综合征(ARDS)肺组织水通道蛋白1(AQP1)表达水平的时效规律以及与肺损伤程度、经典炎症指标的相关性,旨在为ARDS寻找新的生物标志物及干预靶点。方法脂多糖(LPS)滴鼻制备ARDS小鼠模型,每日称量小鼠体质量,取6 h、1 d、3 d和7 d四个时间点肺组织和肺泡灌洗液,HE分析肺组织病理变化,ELISA和qPCR检测炎症因子IL-1β、IL-6和TNF-α的表达水平,流式细胞术分析中性粒细胞、肺泡巨噬细胞、嗜酸性粒细胞、单核细胞等天然免疫细胞数量及比例,qPCR和Western blotting检测AQP1 mRNA和蛋白表达水平,Pearson法分析AQP1与炎性细胞相关性。结果LPS刺激6 h后小鼠肺组织有炎性细胞浸润,至第3日出现严重肺损伤,第7日有所恢复;炎症因子IL-1β、IL-6和TNF-αmRNA和蛋白水平持续升高至第3日,第7日降低(P<0.05);中性粒细胞比例显著升高,第7日降低,肺泡巨噬细胞和嗜酸性粒细胞、单核细胞比例持续降低至第3日,第7日升高(P<0.05);AQP1 mRNA和蛋白表达水平持续降低(P<0.05);AQP1表达水平与四种炎性细胞显著相关(P<0.01)。结论ARDS肺组织AQP1表达水平与肺损伤程度及炎症指标呈显著负相关,可作为肺损伤的新生物标志物,进一步揭示AQP1可能扮演的保护性角色具有潜在临床价值。 展开更多
关键词 急性呼吸窘迫综合征 水通道蛋白1 脂多糖 炎性细胞 炎性因子
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“双高计划”背景下工科类人才培养创新研究——以画法几何及机械制图课程改革为例
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作者 马超 李玉宏 +2 位作者 许世鹏 闫旺 陈维铅 《中阿科技论坛(中英文)》 2024年第7期137-141,共5页
《关于实施中国特色高水平高职学校和专业建设计划的意见》(以下简称“双高计划”)对新时代高职教育发展提出了要求、指明了方向、明确了任务。“双高计划”以立德树人为根本、以提升质量为核心、以内涵式发展为主线、以世界水平的高职... 《关于实施中国特色高水平高职学校和专业建设计划的意见》(以下简称“双高计划”)对新时代高职教育发展提出了要求、指明了方向、明确了任务。“双高计划”以立德树人为根本、以提升质量为核心、以内涵式发展为主线、以世界水平的高职学校和专业群建设为抓手,从产教融合、师资建设、服务水平等多维度提升高职教育发展质量,有针对性地破解高职教育在发展中的瓶颈问题,以形成中国特色职业教育的发展模式,打造中国职业教育的国际品牌。文章以画法几何及机械制图课程为例,探讨“双高计划”背景下课程改革思路和实践教学方法,提出通过调整课程内容、创新教学手段、拓展实践环节等措施,来提高学生的实践能力和职业素养,提升画法几何及机械制图课程改革成效。 展开更多
关键词 “双高计划” 画法几何及机械制图 高职教育
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基于改进德尔菲法的公路货运站站级标准划分研究
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作者 闫旺 谭晓伟 《内蒙古公路与运输》 2024年第2期45-48,共4页
在分析现有公路货运站站级标准划分依据以及国内外中小企业划分依据的基础上,从设施设备、资产、经营状况、管理及服务4个方面,初步选取了10个指标作为公路货运站站级标准划分依据,采用问卷调查的方式,选择16名专家对初步提出的10个指... 在分析现有公路货运站站级标准划分依据以及国内外中小企业划分依据的基础上,从设施设备、资产、经营状况、管理及服务4个方面,初步选取了10个指标作为公路货运站站级标准划分依据,采用问卷调查的方式,选择16名专家对初步提出的10个指标的重要性进行评判,分别采用模糊德尔菲法和灰色德尔菲法分析10个指标的相对重要性,对比分析两种不确定性方法的计算结果,并采用交集的原则确定公路货运站站级标准划分依据。研究结果表明:选取占地面积、处理能力和注册资金3个指标作为公路货运站站级标准划分依据是相对科学、合理的。 展开更多
关键词 交通运输 公路货运站 站级标准划分 模糊德尔菲法 灰色德尔菲法
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Exosomes derived from microglia overexpressing miR-124-3p alleviate neuronal endoplasmic reticulum stress damage after repetitive mild traumatic brain injury
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作者 yan wang Dai Li +12 位作者 Lan Zhang Zhenyu Yin Zhaoli Han Xintong Ge Meimei Li Jing Zhao Shishuang Zhang yan Zuo Xiangyang Xiong Han Gao Qiang Liu Fanglian Chen Ping Lei 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第9期2010-2018,共9页
We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain injury.However,its impact on neuronal endoplasmic reticulum stress following repet... We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain injury.However,its impact on neuronal endoplasmic reticulum stress following repetitive mild traumatic brain injury remains unclear.In this study,we first used an HT22 scratch injury model to mimic traumatic brain injury,then co-cultured the HT22 cells with BV2 microglia expressing high levels of miR-124-3p.We found that exosomes containing high levels of miR-124-3p attenuated apoptosis and endoplasmic reticulum stress.Furthermore,luciferase reporter assay analysis confirmed that miR-124-3p bound specifically to the endoplasmic reticulum stress-related protein IRE1α,while an IRE1αfunctional salvage experiment confirmed that miR-124-3p targeted IRE1αand reduced its expression,thereby inhibiting endoplasmic reticulum stress in injured neurons.Finally,we delivered microglia-derived exosomes containing miR-124-3p intranasally to a mouse model of repetitive mild traumatic brain injury and found that endoplasmic reticulum stress and apoptosis levels in hippocampal neurons were significantly reduced.These findings suggest that,after repetitive mild traumatic brain injury,miR-124-3 can be transferred from microglia-derived exosomes to injured neurons,where it exerts a neuroprotective effect by inhibiting endoplasmic reticulum stress.Therefore,microglia-derived exosomes containing miR-124-3p may represent a novel therapeutic strategy for repetitive mild traumatic brain injury. 展开更多
关键词 apoptosis C/EBP homologous protein endoplasmic reticulum stress EXOSOME inositol-requiring enzyme MICROGLIA miR-124-3p neuron repetitive mild traumatic brain injury X-box binding protein 1
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基于虚拟仿真技术的汽车NVH实验教学研究 被引量:1
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作者 吴昱东 严旺 文广 《时代汽车》 2024年第11期91-93,共3页
随着汽车工业的快速发展,人们对汽车产品驾乘体验要求的不断提高,汽车噪声、振动和声振舒适性(NVH)日益受到关注。汽车NVH实验教学已成为汽车工程领域教育的重要内容,传统的汽车NVH实验教学模式存在成本高昂、时间长、受环境影响大、安... 随着汽车工业的快速发展,人们对汽车产品驾乘体验要求的不断提高,汽车噪声、振动和声振舒适性(NVH)日益受到关注。汽车NVH实验教学已成为汽车工程领域教育的重要内容,传统的汽车NVH实验教学模式存在成本高昂、时间长、受环境影响大、安全风险高等问题,往往难以满足教学需求。虚拟仿真技术为汽车NVH实验教学提供了新的思路,虚拟仿真技术在汽车NVH工程领域应用广泛,将其引入汽车NVH实验教学,不仅能够提供更加灵活和安全的实验环境,而且能够提供更加直观和深入的学习体验,有助于学生更好地掌握NVH理论与实践知识。 展开更多
关键词 汽车NVH 虚拟仿真 实验教学 汽车工程
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Establishing VIGS and CRISPR/Cas9 techniques to verify RsPDS function in radish
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作者 Jiali Ying yan wang +10 位作者 Liang Xu Tiaojiao Qin Kai Xia Peng Zhang Yinbo Ma Keyun Zhang Lun wang Junhui Dong Lianxue Fan Yuelin Zhu Liwang Liu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2024年第5期1557-1567,共11页
Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification... Virus-induced gene silencing(VIGS)and clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)systems are effective technologies for rapid and accurate gene function verification in modern plant biotechnology.However,the investigation of gene silencing and editing in radish remains limited.In this study,a bleaching phenotype was generated through the knockdown of RsPDS using tobacco rattle virus(TRV)-and turnip yellow mosaic virus(TYMV)-mediated gene silencing vectors.The TYMV-mediated gene silencing efficiency was higher than the TRV-based VIGS system in radish.The expression level of RsPDS was significantly inhibited using VIGS in'NAU-067'radish leaves.The rootless seedlings of‘NAU-067'were infected with Agrobacterium rhizogenes using the 2300GN-Ubi-RsPDS-Cas9 vector with two target sequences.Nine adventitious roots were blue with GUs staining,and four of these adventitious roots were edited at target sequence 1 of the RsPDS gene as indicated by Sanger sequencing.Furthermore,albino lines were generated with A.tumefaciens-mediated transformation of radish cotyledons.Five base substitutions and three base deletions occurred at target sequence 2 in Line 1,and three base insertions and three base substitutions occurred at target sequence 1 in Line 2.This study shows that VIGS and CRISPR/Cas9 techniques can be employed to precisely verify the biological functions of genes in radish,which will facilitate the genetic improvement of vital horticultural traits in radish breeding programs. 展开更多
关键词 Raphanus sativus L. VIGS CRISPR/Cas9 Agrobacterium rhizogenes A.tumefaciens RsPDS
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Metabotropic glutamate receptors(mGluRs)in epileptogenesis:an update on abnormal mGluRs signaling and its therapeutic implications
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作者 Leyi Huang Wenjie Xiao +7 位作者 yan wang Juan Li Jiaoe Gong Ewen Tu Lili Long Bo Xiao Xiaoxin yan Lily Wan 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期360-368,共9页
Epilepsy is a neurological disorder characterized by high morbidity,high recurrence,and drug resistance.Enhanced signaling through the excitatory neurotransmitter glutamate is intricately associated with epilepsy.Meta... Epilepsy is a neurological disorder characterized by high morbidity,high recurrence,and drug resistance.Enhanced signaling through the excitatory neurotransmitter glutamate is intricately associated with epilepsy.Metabotropic glutamate receptors(mGluRs)are G protein-coupled receptors activated by glutamate and are key regulators of neuronal and synaptic plasticity.Dysregulated mGluR signaling has been associated with various neurological disorders,and numerous studies have shown a close relationship between mGluRs expression/activity and the development of epilepsy.In this review,we first introduce the three groups of mGluRs and their associated signaling pathways.Then,we detail how these receptors influence epilepsy by describing the signaling cascades triggered by their activation and their neuroprotective or detrimental roles in epileptogenesis.In addition,strategies for pharmacological manipulation of these receptors during the treatment of epilepsy in experimental studies is also summarized.We hope that this review will provide a foundation for future studies on the development of mGluR-targeted antiepileptic drugs. 展开更多
关键词 antiepileptic drugs EPILEPTOGENESIS metabotropic glutamate receptors(mGluRs) signal pathways therapeutic potentials
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