BACKGROUND Diabetes mellitus type 2(T2DM)is formed by defective insulin secretion with the addition of peripheral tissue resistance of insulin action.It has been affecting over 400 million people all over the world.AI...BACKGROUND Diabetes mellitus type 2(T2DM)is formed by defective insulin secretion with the addition of peripheral tissue resistance of insulin action.It has been affecting over 400 million people all over the world.AIM To explore the pathogenesis of T2DM and to develop and implement new prevention and treatment strategies for T2DM.METHODS Receiver operating characteristic(ROC)curve analysis was used to conduct diagnostic markers.The expression level of genes was determined by reverse transcription-PCR as well as Western blot.Cell proliferation assays were performed by cell counting kit-8(CCK-8)tests.At last,T2DM mice underwent Roux-en-Y gastric bypass surgery.RESULTS We found that NPAS2 was significantly up-regulated in isletβcell apoptosis of T2DM.The ROC curve revealed that NPAS2 was capable of accurately diagnosing T2DM.NPAS2 overexpression did increase the level of KANK1.In addition,the CCK-8 test revealed knocking down NPAS2 and KANK1 increased the proliferation of MIN6 cells.At last,we found that gastric bypass may treat type 2 diabetes by down-regulating NPAS2 and KANK1.CONCLUSION This study demonstrated that NPAS2 inducedβcell dysfunction by regulating KANK1 expression in type 2 diabetes,and it may be an underlying therapy target of T2DM.展开更多
A three-dimensional numerical model coupling the macrosegregation and magnetohydrodynamic simulations was developed to investigate the effects of electromagnetic stirring(EMS)on the macrosegregation.The results show t...A three-dimensional numerical model coupling the macrosegregation and magnetohydrodynamic simulations was developed to investigate the effects of electromagnetic stirring(EMS)on the macrosegregation.The results show that a significant swirling flow was induced by the in-mold EMS,which further changed the shape of the solidification shell and homogenized the solute elements in mold.However,the effects were only confined to the initial billet shell.The improvement in centerline segregation was observed with the usage of the final EMS(F-EMS),which led to the forced convection at the final solidification stage.The solute elements in the mushy zone were significantly even,with the maximum segregation degree of solute C reducing from 1.311 to 1.237.In addition,the effects of the stirrer positions and currents of F-EMS on the macrosegregation alleviation were numerically studied.Different values of centerline segregation were predicted with various stirrer positions and currents,and there is an optimum stirrer parameter to obtain the best macrosegregation alleviation.In the experimental conditions,the optimum position was about 7 m away from meniscus,and the optimum current was 300 A.展开更多
To investigate the transient transport and entrapment of argon bubbles and inclusions simultaneously during continuous casting,a 3D large eddy simulation model coupling molten steel flow,solidification and particle mo...To investigate the transient transport and entrapment of argon bubbles and inclusions simultaneously during continuous casting,a 3D large eddy simulation model coupling molten steel flow,solidification and particle motion was constructed.In this model,momentum transfer between molten steel and argon bubbles was performed by two-way coupling.The predicted results indicate that argon bubble injection changed the flow pattern of molten steel and the inclusion motion in the liquid pool.Consequently,the inclusion capture near the solidifying front was changed.In addition,measurements of bubbles and inclusions in the obtained samples were performed by methods of optical microscope examination and galvanostatic electrolysis.The results show a favorable agreement between the model predictions and the measured results,which validate the mathematical model.Furthermore,the predicted results of the inclusion entrapment in the case with argon bubble injection are more compatible with the measurements than those in the case without argon bubble injection.展开更多
基金Supported by Natural Science Foundation of Heilongjiang Province,No.LH2021H105.
文摘BACKGROUND Diabetes mellitus type 2(T2DM)is formed by defective insulin secretion with the addition of peripheral tissue resistance of insulin action.It has been affecting over 400 million people all over the world.AIM To explore the pathogenesis of T2DM and to develop and implement new prevention and treatment strategies for T2DM.METHODS Receiver operating characteristic(ROC)curve analysis was used to conduct diagnostic markers.The expression level of genes was determined by reverse transcription-PCR as well as Western blot.Cell proliferation assays were performed by cell counting kit-8(CCK-8)tests.At last,T2DM mice underwent Roux-en-Y gastric bypass surgery.RESULTS We found that NPAS2 was significantly up-regulated in isletβcell apoptosis of T2DM.The ROC curve revealed that NPAS2 was capable of accurately diagnosing T2DM.NPAS2 overexpression did increase the level of KANK1.In addition,the CCK-8 test revealed knocking down NPAS2 and KANK1 increased the proliferation of MIN6 cells.At last,we found that gastric bypass may treat type 2 diabetes by down-regulating NPAS2 and KANK1.CONCLUSION This study demonstrated that NPAS2 inducedβcell dysfunction by regulating KANK1 expression in type 2 diabetes,and it may be an underlying therapy target of T2DM.
基金the China Postdoctoral Science Foundation(No.2018M642308)Jiangsu Planned Projects for Postdoctoral Research Funds(No.2018K068C).
文摘A three-dimensional numerical model coupling the macrosegregation and magnetohydrodynamic simulations was developed to investigate the effects of electromagnetic stirring(EMS)on the macrosegregation.The results show that a significant swirling flow was induced by the in-mold EMS,which further changed the shape of the solidification shell and homogenized the solute elements in mold.However,the effects were only confined to the initial billet shell.The improvement in centerline segregation was observed with the usage of the final EMS(F-EMS),which led to the forced convection at the final solidification stage.The solute elements in the mushy zone were significantly even,with the maximum segregation degree of solute C reducing from 1.311 to 1.237.In addition,the effects of the stirrer positions and currents of F-EMS on the macrosegregation alleviation were numerically studied.Different values of centerline segregation were predicted with various stirrer positions and currents,and there is an optimum stirrer parameter to obtain the best macrosegregation alleviation.In the experimental conditions,the optimum position was about 7 m away from meniscus,and the optimum current was 300 A.
基金The authors gratefully express their appreciation to the National Natural Science Foundation of China(51834002)Fundamental Research Funds for the Central Universities(FRF-AT-20-05)for sponsoring this work.
文摘To investigate the transient transport and entrapment of argon bubbles and inclusions simultaneously during continuous casting,a 3D large eddy simulation model coupling molten steel flow,solidification and particle motion was constructed.In this model,momentum transfer between molten steel and argon bubbles was performed by two-way coupling.The predicted results indicate that argon bubble injection changed the flow pattern of molten steel and the inclusion motion in the liquid pool.Consequently,the inclusion capture near the solidifying front was changed.In addition,measurements of bubbles and inclusions in the obtained samples were performed by methods of optical microscope examination and galvanostatic electrolysis.The results show a favorable agreement between the model predictions and the measured results,which validate the mathematical model.Furthermore,the predicted results of the inclusion entrapment in the case with argon bubble injection are more compatible with the measurements than those in the case without argon bubble injection.
