Migratory properties of cultured olfactory ensheathing cells by single-cell migration assay

嗅觉的 ensheathing 房间(OEC ) 是有 axonal 的 glial 房间的一种唯一的类型支持生长的性质。OEC 移植作为 axonal 损害和 demyelinating 疾病的有希望的试验性的治疗出现了。然而， OEC 的一些基本细胞的性质仍然保持不清楚。在这研究，我们发现不同 OEC 候补选手人口基于单个孤立的房间的微速摄影的成像展出了不同迁移的性质，可能由于他们的不同细胞骨架组织。而且， OEC 潜水艇人口在单个房间的迁居试金显示了不同吸引人的迁移的回答到 lysophosphatidic 酸(LPA ) 的一个坡度。最后，我们发现了人口自发地转变了成对方的那个 OEC 代用品。一起，这些结果示威，第一次到我们的知识，不同 OEC 潜水艇人口显示不同迁移的性质试管内并且提供新证据当一个单个房间与韧性的功能的显型打字，支持 OEC 的观点。

Implementation of high-fidelity neutronics and thermal–hydraulic coupling calculations in HNET

To perform nuclear reactor simulations in a more realistic manner,the coupling scheme between neutronics and thermal-hydraulics was implemented in the HNET program for both steady-state and transient conditions.For simplicity,efficiency,and robustness,the matrixfree Newton/Krylov(MFNK)method was applied to the steady-state coupling calculation.In addition,the optimal perturbation size was adopted to further improve the convergence behavior of the MFNK.For the transient coupling simulation,the operator splitting method with a staggered time mesh was utilized to balance the computational cost and accuracy.Finally,VERA Problem 6 with power and boron perturbation and the NEACRP transient benchmark were simulated for analysis.The numerical results show that the MFNK method can outperform Picard iteration in terms of both efficiency and robustness for a wide range of problems.Furthermore,the reasonable agreement between the simulation results and the reference results for the NEACRP transient benchmark verifies the capability of predicting the behavior of the nuclear reactor.

Tricuspid Valve Reconstruction in a Patient with Ventricular Septal Defect and Subacute Endocarditis

Objective Tricuspid valve reconstruction was advocated as the operative method for the treatment of tricus-pid valve endocarditis recently. Many people accept that valve replacement therapy should be performed if more than two valve leaflets are involved. The aim of the study to discuss if reconstructive surgery could be done to treat two valve leaflets involved in tricuspid valve endocarditis. Methods A 17-year-old boy with ventricular septal defect (VSD) and tricuspid valve subacute endocarditis was surgical treated through extra-corporeal circulation. two-thirds of the defective septal cusp, and half of the defective anterior cusp were ex-cised during operation. The tricuspid valve was reconstructed with autologous pericardial strip, cusp com-missuroplasty and 4-0 prolene sutures made as chordae tendineaes. VSD was repaired using a pericardial patch. Results The patient was discharged post-operation with excellent restoration of the tricuspid valve ac-tivity. Cardiac ultrasound revealed normal tricuspid valve activity and low degree of regurgitation two years follow-up. Conclusions It seems that tricuspid valve reconstruction could be performed for two defective leaflets or half of the tricuspid valve.

Desflurane anesthesia compared with total intravenous anesthesia on anesthesia-controlled operating room time in ambulatory surgery following strabotomy:a randomized controlled study

Background:Ophthalmic ambulatory surgery is preferred to be performed under general anesthesia either by total intravenous anesthesia(TIVA)or by inhalational anesthesia to increase the patient comfort.However,anesthesia-controlled time(ACT)can cause increased non-operative operating room(OR)time which may adversely affect the ORs efficiency.This study was aimed to compare the ACT of desflurane with that of propofol-remifentanil in strabismus ambulatory surgery.Methods:From November 2016 to December 2017,a total of 200 strabismus patients(aged 18-60 years old,and scheduled for elective ambulatory surgery at Zhongshan Ophthalmic Center)were randomly assigned to receive either propofol-based TIVA(group TIVA)or desflurane anesthesia(group DES)for maintenance of anesthesia.The primary outcome was the extubation time.Secondary outcomes included surgical time,anesthetic time,OR exit time,and Phase I and II recovery time.The intraoperative incidences of hypotension,bradycardia and oculocardiac reflex(OCR),and the incidences of any post-operative complications were recorded.Mann-Whitney U test and Chi-square or Fisher exact tests were used to compare the two groups.Results:We found that the extubation time(5.5[3.9-7.0]vs.9.7[8.5-11.4]min,P<0.001)and the incidence of prolonged time to extubation(0 vs.6%,P=0.029)in the DES group were significantly decreased compared with those in the TIVA group.The patients in the DES group displayed shorter OR exit time as compared with that in the TIVA group(7.3[5.5-8.7]vs.10.8[9.3-12.3]min,P<0.001).The patients using desflurane exhibited more stable hemodynamics during surgery than the patients using propofol-based TIVA,as demonstrated by lower incidences of hypotension(1%vs.22%,P<0.001),bradycardia(2%vs.13%,P=0.002),and OCR(17%vs.44%,P<0.001).Conclusion:DES enhanced the ophthalmic OR efficiency by reducing the extubation time and OR exit time,and provided more stable hemodynamics intra-operatively than TIVA in patients undergoing strabismus ambulatory surgery.Trial registration:ClinicalTrials.gov,No.NCT02922660;https://clinicaltrials.gov/ct2/show/NCT02922660?id=NCT02922660&draw=2&rank=1.

A Facile Way to Large-scale Production of Few-layered Graphene via Planetary Ball Mill

Abstract In the field of polymer/graphene nanocomposites, massive production and commercial availability of graphene are essential. Exfoliation of graphite to obtain graphene is one of the most promising ways to large-scale production at extremely low cost. In this work we illustrate a facile strategy for mass production of few-layered （≤ 10） graphene （FLG） via the newly explored ball milling. The achieved FLG concentration was determined by UV/Vis spectroscopy. The formation of FLG was proved by measuring the flake thickness by atomic force microscopy （AFM）. Further Raman spectral studies indicated that the crystal structure of exfoliated flakes was preserved satisfactorily during this shear-force dominating process. To increase the maximum concentration obtainable, it＇s critical to make a good parameter assessment. N-methylpyrrolidone （NMP） was used as a dispersing medium and the effect of milling parameters was systematically and quantitatively investigated, thus providing a criterion to optimize the milling process. We established the optimal values for solvent volume and initial weight of graphite. As for milling time, the production of FLG was enhanced with continuous milling according to the power law, but not linearly with increasing milling time. Moreover, the possible mechanism involved in milling process was also explored. Our work provides a simple method for graphite exfoliation and has great potential for improving thermal and electrical conductivity of polymer composites in the fields of engineering.

Size Distribution and Anisotropy of the Minor Phase Droplets in Polypropylene/Ethylene-Octene Copolymer Blends: Effects of Shear and Component Miscibility

In this work, polypropylene （PP）/octene-ethylene copolymer （POE） blends were injection-molded using the so- called dynamic packing injection technique, which imposed oscillatory shear on the gradually cooling melt during the packing solidification stage. In this way, the effect of shear on the size distribution and anisotropy of the minor phase droplets could be investigated. Besides, by using two kinds of POE with different octene contents, the effect of component miscibility was also studied. The results show that the droplet size is mainly determined by composition and miscibility, and droplet anisotropy is mainly determined by droplet size and shear. Most importantly, under the same processing condition, droplet anisotropy increases with droplet size, and there seems a linear fit between them, disregarding the miscibility factor. These results may provide guidance for preparing polymer blends with desired properties by tailoring their phase morphologies.