The genus Synalpheus （Decapoda： Alpheidae） from China seas is reviewed. Thirty one species belonging to this genus are described and illustrated in the present paper, including 4 new species and 6 newly recorded sp...The genus Synalpheus （Decapoda： Alpheidae） from China seas is reviewed. Thirty one species belonging to this genus are described and illustrated in the present paper, including 4 new species and 6 newly recorded species from the China seas as following： S. brevrostrus sp. nov., S. jinqingensis sp. nov., S. liui sp. nov., S. tonkinensis sp. nov., S. biunguiculatus （Stimpson, 1860）, S. gracilirostris de Man, 1910, S. iphinoe de Man, 1909, S. lophodactylus Coutiere, 1908, S. modestus de Man, 1909 and S. mortenseni Banners, 1985. A key to 31 species of the genus Synalpheus from China seas is provided.展开更多
Objective:The aim is to study the effects of metformin on the expression of 70 kDa ribosomal protein S6 kinase(P70S6k),insulin receptor substrate 1(IRS-1),and IRS-1Ser307 phosphorylation in human luteinized granulosa ...Objective:The aim is to study the effects of metformin on the expression of 70 kDa ribosomal protein S6 kinase(P70S6k),insulin receptor substrate 1(IRS-1),and IRS-1Ser307 phosphorylation in human luteinized granulosa cells.Methods:Granulosa cells in the experimental group were cultured in M199 medium containing 0.1 mmol/L metformin for 24 h and those in control group were cultured in M199 medium.The expression levels of P70S6k and IRS-1 mRNA were detected by reverse-transcriptiom polymerase chain reaction(RT-PCR)and real-time PCR.P70S6k,IRS-1,p-ser307-IRS-1,and p-thr389-P70S6k protein expression levels were detected by immunofluorescence and western blotting.Results:P70S6k mRNA level was higher and IRS-1 was significantly lower in the experimental group than those in the control group.IRS-1 and p-ser307-IRS-1 were expressed in cell plasma,and P70S6k and p-thr389-P70S6k were expressed in cell nucleus.The results of Western blot analysis indicated that the expression levels of P70S6k,p-thr389-P70S6k,IRS-1,and p-ser307-IRS-1 proteins had significant difference between the experimental group and the control group.Compared to the control group,the relative intensity illustrated that the expression levels of P70S6K and p-thr389-P70S6k significantly increased in the experimental group;however,those of IRS-1 and p-ser307-IRS-1 proteins significantly decreased.Conclusion:Metformin can inhibit the P70S6k mRNA and protein expression levels in the granulosa cells and improve insulin sensitivity by regulating IRS-1 expression through Akt/P70S6k/IRS-1-dependent pathway.展开更多
基金supported by the National Natural Science Foundation of China(31172054)partly supported by the project of the S&T basic work from the Ministry of Science and Technology of China(2014FY110500,2015FY210300)
文摘The genus Synalpheus （Decapoda： Alpheidae） from China seas is reviewed. Thirty one species belonging to this genus are described and illustrated in the present paper, including 4 new species and 6 newly recorded species from the China seas as following： S. brevrostrus sp. nov., S. jinqingensis sp. nov., S. liui sp. nov., S. tonkinensis sp. nov., S. biunguiculatus （Stimpson, 1860）, S. gracilirostris de Man, 1910, S. iphinoe de Man, 1909, S. lophodactylus Coutiere, 1908, S. modestus de Man, 1909 and S. mortenseni Banners, 1985. A key to 31 species of the genus Synalpheus from China seas is provided.
基金supported by the Science Technology Research Project of Higher Education of Ningxia Province(NGY2016125)the Open Project of Key Laboratory of Fertility Preservation and Maintenance,Ministry of Education(XY201518)。
文摘Objective:The aim is to study the effects of metformin on the expression of 70 kDa ribosomal protein S6 kinase(P70S6k),insulin receptor substrate 1(IRS-1),and IRS-1Ser307 phosphorylation in human luteinized granulosa cells.Methods:Granulosa cells in the experimental group were cultured in M199 medium containing 0.1 mmol/L metformin for 24 h and those in control group were cultured in M199 medium.The expression levels of P70S6k and IRS-1 mRNA were detected by reverse-transcriptiom polymerase chain reaction(RT-PCR)and real-time PCR.P70S6k,IRS-1,p-ser307-IRS-1,and p-thr389-P70S6k protein expression levels were detected by immunofluorescence and western blotting.Results:P70S6k mRNA level was higher and IRS-1 was significantly lower in the experimental group than those in the control group.IRS-1 and p-ser307-IRS-1 were expressed in cell plasma,and P70S6k and p-thr389-P70S6k were expressed in cell nucleus.The results of Western blot analysis indicated that the expression levels of P70S6k,p-thr389-P70S6k,IRS-1,and p-ser307-IRS-1 proteins had significant difference between the experimental group and the control group.Compared to the control group,the relative intensity illustrated that the expression levels of P70S6K and p-thr389-P70S6k significantly increased in the experimental group;however,those of IRS-1 and p-ser307-IRS-1 proteins significantly decreased.Conclusion:Metformin can inhibit the P70S6k mRNA and protein expression levels in the granulosa cells and improve insulin sensitivity by regulating IRS-1 expression through Akt/P70S6k/IRS-1-dependent pathway.
