期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Preventive and therapeutic effects of NF-kappaB inhibitor curcumin in rats colitis induced by trinitrobenzene sulfonic: acid 被引量:19
1
作者 yan-tingjian Guo-FengMai +3 位作者 Ji-DeWang Ya-LiZhang Rong-ChengLuo Yong-XinFang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第12期1747-1752,共6页
AIM: To ascertain the molecule mechanism of nuclear factor-KB (NF-κB) inhibitor curcumin preventive and therapeutic effects in rats' colitis induced by trinitrobenzene sulfonic acid (TNBS). METHODS: Sixty rats wi... AIM: To ascertain the molecule mechanism of nuclear factor-KB (NF-κB) inhibitor curcumin preventive and therapeutic effects in rats' colitis induced by trinitrobenzene sulfonic acid (TNBS). METHODS: Sixty rats with TNBS-induced colitis were treated with 2.0% curcumin in the diet. Thirty positive control rats were treated with 0.5% sulfasalazine (SASP). Thirty negative control rats and thirty model rats were treated with general diet. Changes of body weight together with histological scores were evaluated. Survival rates were also evaluated. Cell nuclear NF-κB activity in colonic mucosa was evaluated by using electrophoretic mobility shift assay. Cytoplasmic IκB protein in colonic mucosa was detected by using Western Blot analysis. Cytokine messenger expression in colonic tissue was assessed by using semiquantitative reverse-transcription polymerase chain reaction. RESULTS: Treatment with curcumin could prevent and treat both wasting and histopathologic signs of rats with TNBS-induced intestinal inflammation. In accordance with these findings, NF-κB activation in colonic mucosa was suppressed in the curcumin-treated groups. Degradations of cytoplasmic IκB protein in colonic mucosa were blocked by curcumin treatment. Proinflammatory cytokine messenger RNA expression in colonic mucosa was also suppressed. CONCLUSION: This study shows that NF-κB inhibitor curcumin could prevent and improve experimental colitis in murine model with inflammatory bowel disease (IBD). The findings suggest that NF-κB inhibitor curcumin could be a potential target for the patients with IBD. 展开更多
关键词 IBD CURCUMIN TNBS NF-κB
下载PDF
A novel method for preparation of tissue microarray 被引量:3
2
作者 Han-LeiDan Ya-LiZhang +9 位作者 YanZhang Ya-DongWang Zuo-ShengLai Yu-JieYang Hai-HongCui yan-tingjian JianGeng Yan-QingDing Chun-HaiGuo Dian-YuanZhou 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第4期579-582,共4页
AIM:To improve the technique of tissue microarray (tissue chip).METHODS: A new tissue microarraying method was invented with a common microscope installed with a special holing needle, a sampling needle, and a special... AIM:To improve the technique of tissue microarray (tissue chip).METHODS: A new tissue microarraying method was invented with a common microscope installed with a special holing needle, a sampling needle, and a special box fixing paraffin blocks on the microscope slide carrier. With the movement of microscope tube and objective stage on vertical and cross dimensions respectively, the holing procedure on the recipient paraffin blocks and sampling procedure of core tissue biopsies taken from the donor blocks were performed with the refitted microscope on the same platform.The precise observation and localization of representative regions in the donor blocks were also performed with the microscope equipped with a stereoscope.RESULTS: Highly-qualified tissue chips of colorectal tumors were produced by a new method, which simplified the conventional microarraying procedure, and was more convenient and accurate than that employing the existing tissue microarraying instruments.CONCLUSION:Using the refitted common microscope to produce tissue microarray is a simple, reliable, cost-effective and well-applicable technique. 展开更多
关键词 组织微阵列技术 组织芯片 显微镜 样本处理 实验医学
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部