Previous studies have shown that transcranial pulse current stimulation(tPCS) can increase cerebral neural plasticity and improve patients' locomotor function.However, the precise mechanisms underlying this effect...Previous studies have shown that transcranial pulse current stimulation(tPCS) can increase cerebral neural plasticity and improve patients' locomotor function.However, the precise mechanisms underlying this effect remain unclear.In the present study, rat models of stroke established by occlusion of the right cerebral middle artery were subjected to tPCS, 20 minutes per day for 7 successive days.tPCS significantly reduced the Bederson score, increased the foot print area of the affected limbs, and reduced the standing time of affected limbs of rats with stroke compared with that before intervention.Immunofluorescence staining and western blot assay revealed that tPCS significantly increased the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra.This finding suggests that tPCS can improve the locomotor function of rats with stroke by regulating the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra.These findings may provide a new method for the clinical treatment of poststroke motor dysfunction and a theoretical basis for clinical application of tPCS.The study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine of China(approval No.PZSHUTCM190315003) on February 22, 2019.展开更多
In recent years,the brain mechanism research speeds up for the effect of acupuncture intervention on chronic pain.Currently,in the relevant articles,the brain mechanism research in chronic pain focuses on musculoskele...In recent years,the brain mechanism research speeds up for the effect of acupuncture intervention on chronic pain.Currently,in the relevant articles,the brain mechanism research in chronic pain focuses on musculoskeletal system diseases,in which,the rigorous controlled designs has been adopted in the most of researches and the effect verification has been predominated as the research objective.The corresponding results indicate that the brain mechanism of acupuncture effect is mediated by brain area,nerve circuit or brain network related with sensory,motor,cognitive and emotional functions.In this paper,the prospective thinking has been conducted on the dual-directional transfer between the relevant brain mechanism research and clinical research achievement.Moreover,the optimization and innovation of intervention scheme are prospected while the acupuncture intervention is promoted in pain rehabilitation widely in the future.展开更多
基金supported by the National Key R&D Program of China, No.2018 YFC2001600(to CLS)the Shanghai Health Commission Accelerated the Development of Traditional Chinese Medicine Three-Year Action Plan Project, No.ZY(2018-2020)-CCCX-2001-06/2004-05(to CLS)+1 种基金the Program of Shanghai Academic Research Leader, No.19 XD1403600(to CLS)the National Natural Science Foundation of China for the Youth Project, No.81704163(to JJZ)。
文摘Previous studies have shown that transcranial pulse current stimulation(tPCS) can increase cerebral neural plasticity and improve patients' locomotor function.However, the precise mechanisms underlying this effect remain unclear.In the present study, rat models of stroke established by occlusion of the right cerebral middle artery were subjected to tPCS, 20 minutes per day for 7 successive days.tPCS significantly reduced the Bederson score, increased the foot print area of the affected limbs, and reduced the standing time of affected limbs of rats with stroke compared with that before intervention.Immunofluorescence staining and western blot assay revealed that tPCS significantly increased the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra.This finding suggests that tPCS can improve the locomotor function of rats with stroke by regulating the expression of microtubule-associated protein-2 and growth-associated protein-43 around the ischemic penumbra.These findings may provide a new method for the clinical treatment of poststroke motor dysfunction and a theoretical basis for clinical application of tPCS.The study was approved by the Animal Use and Management Committee of Shanghai University of Traditional Chinese Medicine of China(approval No.PZSHUTCM190315003) on February 22, 2019.
基金Supported by General Program of National Natural Science Foundation of China No.81874035the Shanghai Municipal Commission of Health and Family Planning of China:ZY(2018-2020)-CCCX-2001-06,ZY(2018-2020)-CCCX-2004-05 and ZY(2018-2020)-FWTX-8002.
文摘In recent years,the brain mechanism research speeds up for the effect of acupuncture intervention on chronic pain.Currently,in the relevant articles,the brain mechanism research in chronic pain focuses on musculoskeletal system diseases,in which,the rigorous controlled designs has been adopted in the most of researches and the effect verification has been predominated as the research objective.The corresponding results indicate that the brain mechanism of acupuncture effect is mediated by brain area,nerve circuit or brain network related with sensory,motor,cognitive and emotional functions.In this paper,the prospective thinking has been conducted on the dual-directional transfer between the relevant brain mechanism research and clinical research achievement.Moreover,the optimization and innovation of intervention scheme are prospected while the acupuncture intervention is promoted in pain rehabilitation widely in the future.
