OBJECTIVE： The stable quality of Chinese herbal medicines is a critical factor for their reliable clinical efficiency. An improved liquid-liquid extraction procedure and a liquid chromatographic method were developed...OBJECTIVE： The stable quality of Chinese herbal medicines is a critical factor for their reliable clinical efficiency. An improved liquid-liquid extraction procedure and a liquid chromatographic method were developed to simultaneously analyze five anthraquinones （aloe-emodin, rhein, emodin, chrysophanol and physcion） in a Chinese traditional hospital preparation, Fuyankang mixture, in order to quantitatively control its quality in a more effective way. METHODS： A more economical and repeatable extraction procedure based on conventional liquid-liquid extraction technique was developed and used to extract five marker components in Fuyankang mixture. These anthraquinones were separated in less than 20 min on a C18 column with methanol and 0.1% phosphoric acid （88：12, v/v） as mobile phase. The method was validated for specificity, precision, spiked recovery and stability. RESULTS： Compared to conventional liquid-liquid extraction, the improved liquid-liquid extraction was found to be more effective for simultaneous extraction of anthraquinones from an aqueous Chinese herbal preparation, especially for hydrophobic compounds. The improved extraction method was successfully applied to determine the content of five marker components in Fuyankang mixture by the means of reverse phase high-performance liquid chromatography. CONCLUSION： The improved extraction procedure may be suitable for routine quality control of Fuyankanq mixture and other traditional preparations at city-level hospitals in China.展开更多
The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted an...The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Eschenchia coil and Staphylococcus aureus. The effect of alkaloids on bacteda growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor （TAM） air isothermal microcalorimeter and pa- rameters such as growth rate constant （p）, peak-time （Trn）, inhibitory ratio （I）, and enhancement ratio （E） were cal- culated. The relationships between the concentration of Aconitum alkaloids and p of E. coil or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coil and had a potentially inhibitory effect on the growth of S. aureus.展开更多
基金supported by the Science and Technology Project of Chinese Traditional Medicine of Gansu,China(No.GZK-2011-72)Agricultural Biotechnology Research and Development Project of Gansu Province,China(No.GNSW-2013-14)
文摘OBJECTIVE： The stable quality of Chinese herbal medicines is a critical factor for their reliable clinical efficiency. An improved liquid-liquid extraction procedure and a liquid chromatographic method were developed to simultaneously analyze five anthraquinones （aloe-emodin, rhein, emodin, chrysophanol and physcion） in a Chinese traditional hospital preparation, Fuyankang mixture, in order to quantitatively control its quality in a more effective way. METHODS： A more economical and repeatable extraction procedure based on conventional liquid-liquid extraction technique was developed and used to extract five marker components in Fuyankang mixture. These anthraquinones were separated in less than 20 min on a C18 column with methanol and 0.1% phosphoric acid （88：12, v/v） as mobile phase. The method was validated for specificity, precision, spiked recovery and stability. RESULTS： Compared to conventional liquid-liquid extraction, the improved liquid-liquid extraction was found to be more effective for simultaneous extraction of anthraquinones from an aqueous Chinese herbal preparation, especially for hydrophobic compounds. The improved extraction method was successfully applied to determine the content of five marker components in Fuyankang mixture by the means of reverse phase high-performance liquid chromatography. CONCLUSION： The improved extraction procedure may be suitable for routine quality control of Fuyankanq mixture and other traditional preparations at city-level hospitals in China.
基金supported by the National Natural Science Foundation of China(No.21203112)the Natural Science Foundation of Shandong Province(No.ZR2012BQ002),China
文摘The metabolic activity of organisms can be measured by recording the heat output using microcalorimetry. In this paper, the total alkaloids in the traditional Chinese medicine Radix Aconiti Lateralis were extracted and applied to Eschenchia coil and Staphylococcus aureus. The effect of alkaloids on bacteda growth was studied by microcalorimetry. The power-time curves were plotted with a thermal activity monitor （TAM） air isothermal microcalorimeter and pa- rameters such as growth rate constant （p）, peak-time （Trn）, inhibitory ratio （I）, and enhancement ratio （E） were cal- culated. The relationships between the concentration of Aconitum alkaloids and p of E. coil or S. aureus were discussed. The results showed that Aconitum alkaloids had little effect on E. coil and had a potentially inhibitory effect on the growth of S. aureus.
