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Codon 249 mutation in exon 7 of p53 gene in plasma DNA:maybe a new early diagnostic marker of hepatocellular carcinoma in Qidong risk area,China 被引量:18
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作者 Xing-HuaHuang Lu-HongSun +5 位作者 Dong-DongLu yansun Li-JieMa Xi-RanZhang JianHuang LongYu 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第4期692-695,共4页
AIM: One of the characteristics of hepatocellular carcinoma (HCC) in Qidong area is the selective mutation resulting in a serine substitution at codon 249 of the p53 gene (1,20),and it has been identified as a 'ho... AIM: One of the characteristics of hepatocellular carcinoma (HCC) in Qidong area is the selective mutation resulting in a serine substitution at codon 249 of the p53 gene (1,20),and it has been identified as a 'hotspot' mutation in heptocellular carcinomas occurring in populations exposed to aflatoxin and with high prevalence of hepatitis B virus carriers (2, 3, 9, 10, 16, 24). We evaluated in this paper whether this 'hotspot' mutation could be detected in cellfree DNA circulating in plasma of patients with hepatocellular carcinoma and cirrhosis in Qidong, China, and tried to illustrate the significance of the detection of this molecular biomarker.METHODS: We collected blood samples from 25hepatocellular carcinoma patients, 20 cirrhotic patients and 30 healthy controls in Qidong area. DNA was extracted and purified from 200 μl of plasma from each sample. The 249ser p53 mutation was detected by restriction digestion analysis and direct sequencing of exon-7 PCR products.RESULTS: We found in exon 7 of p53 gene G→T transversion at the third base of codon 249 resulting 249Arg→249ser mutation in 10/25 (40%) hepatocellular carcinoma cases,4/20 (20%) cirrhotics, and 2/30 (7 %) healthy controls.The adjusted odds ratio for having the mutation was 22.1(95 % CI, 3.2~91.7) for HCC cases compared to controls.CONCLUSION: These data show that the 249ser p53mutation in plasma is strongly associated with hepatocellular carcinoma in Qidong patients. We found this mutation was also detected, although it was at a much lower frequency,in plasma DNA of Qidong cirrhotics and healthy controls;We consider that these findings, together with the usual method of HCC diagnosis, will give more information in early diagnosis of HCC, and 249ser p53 mutation should be developed to a new early diagnostic marker for HCC. 展开更多
关键词 P53基因 血浆 DNA 肝细胞癌 肿瘤标志物 早期诊断 高发地区
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Photopromoted Carbonylation of Alkyl Halides Under Ambient Conditions 被引量:1
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作者 yansun JingMeiYIN +2 位作者 DaBinGAO GuangYunZHOU YingPingJIA 《Chinese Chemical Letters》 SCIE CAS CSCD 2003年第6期575-578,共4页
Photopromoted carbonylation of alkyl halides with carbon monoxide can be carried out under ambient conditions with non-precious transition metal complexes (such as cobalt complexes) catalysts. Our preliminary work sh... Photopromoted carbonylation of alkyl halides with carbon monoxide can be carried out under ambient conditions with non-precious transition metal complexes (such as cobalt complexes) catalysts. Our preliminary work showed that alkyl halides can be transformed into alkene and alkane directly under irradiation, but the esters can not be transformed. It is assumed that the carbonylation of alkyl halides may be proceeded in two ways. 展开更多
关键词 Photopromoted carbonylation alkyl halides carbon monoxide.
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近十年植物入侵生态学重要研究进展 被引量:15
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作者 刘艳杰 黄伟 +6 位作者 杨强 郑玉龙 黎绍鹏 吴昊 鞠瑞亭 孙燕 丁建清 《生物多样性》 CAS CSCD 北大核心 2022年第10期272-288,共17页
外来植物入侵对本地生物多样性、生态安全、社会经济发展和人类健康造成了严重威胁。因此,探究植物入侵机制及其生态效应对我国生态可持续发展具有重要意义,也是植物入侵生态学的主要研究内容。过去10年,生态学家开展了大量研究,取得了... 外来植物入侵对本地生物多样性、生态安全、社会经济发展和人类健康造成了严重威胁。因此,探究植物入侵机制及其生态效应对我国生态可持续发展具有重要意义,也是植物入侵生态学的主要研究内容。过去10年,生态学家开展了大量研究,取得了丰硕成果,为推动入侵植物防控与生物多样性保护提供了理论指导。为深入解析外来植物入侵机制,为其防控管理提供依据,本文从以下3个方面综述了植物入侵生态学重要进展:首先是外来植物生物学特性、生物和非生物环境对植物入侵的调控作用;其次是外来植物入侵对本地生态系统的影响;最后介绍了外来植物入侵的类比研究范式——本地植物入侵,以及多组学技术在入侵生态学研究中的应用。基于这些研究进展,本文展望了该领域的未来发展趋势,包括:(1)研究对象从单一物种扩展到多物种比较;(2)研究地理范围从局域尺度扩大到纬度梯度格局;(3)入侵机制从单一理论验证到综合阐释多个假说。 展开更多
关键词 生物互作 生物入侵 多样性 生态学效应 全球变化 入侵机制
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