Objective The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells. Methods HL-60 cells were treated for 24, 48, 72 h with various concentr...Objective The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells. Methods HL-60 cells were treated for 24, 48, 72 h with various concentrations ethanolic extracts of propolis(0, 50, 100, and 200 μg/m L). The proliferation of HL-60 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. Subsequently, Hochest 33258 staining and terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) were used to test the apoptosis of HL-60 cells. We observed the expression levels of Bax and Bcl-2 in HL-60 cells by immunohistochemistry. Results MTT assay showed that various concentrations of ethanolic extract of propolis had significant inhibitory effect on HL-60 cell proliferation(P < 0.05). Typical morphologic changes could be observed by fluorescence microscope and TUNEL. By immunohistochemistry, we found the expression level of Bax was up-regulated, whereas that of Bc1-2 was down-regulated(P < 0.05). Conclusion Ethanolic extract of propolis inhibits leukemia cell proliferation and induces apoptosis in vitro. Its mechanism may be related to the regulation of Bax and Bcl-2 expression and up-regulation of Bcl-2/Bax ratio.展开更多
The multifunctional films was prepared by blending chitosan and nano-ZnO with purple tomato anthocyanins or black wolfberry anthocyanins.The properties of films functioned by anthocyanins source and nano-ZnO content w...The multifunctional films was prepared by blending chitosan and nano-ZnO with purple tomato anthocyanins or black wolfberry anthocyanins.The properties of films functioned by anthocyanins source and nano-ZnO content were studied.It was found purple tomato anthocyanins showed more significant color change against pH than black wolfberry anthocyanins.The nano-ZnO were widely dispersed in matrix and enhanced the compatibility of anthocyanins with chitosan.However,the anthocyanins source influenced the properties of the films more slightly than nano-ZnO addition.The tensile strength,antioxidant and antibacterial effects of the chitosan films dramatically increased after cooperated by nano-ZnO and anthocyanins,which also enhanced with increase of nano-ZnO content,whereas the elongation at break of the composite films decreased.Especially,the anthocyanin and nano-ZnO promoted the antibacterial activity of films synergistically.Composite films made from black wolfberry anthocyanins exhibited higher mechanical performance than those made from purple tomato anthocyanins but weaker antibacterial effects.The purple tomato anthocyanins/chitosan and nano-ZnO/purple tomato anthocyanins/chitosan films effectively reflected pork spoilage,changing their colors from dark green to brown,indicating the potential for applications in active and intelligent food packaging.展开更多
Type 2 diabetes(T2D)is characterized by the malfunction of pancreaticβcells.Susceptibility and pathogenesis of T2D can be affected by multiple factors,including sex differences.However,the mechanisms underlying sex d...Type 2 diabetes(T2D)is characterized by the malfunction of pancreaticβcells.Susceptibility and pathogenesis of T2D can be affected by multiple factors,including sex differences.However,the mechanisms underlying sex differences in T2D susceptibility and pathogenesis remain unclear.Using single-cell RNA sequencing(scRNA-seq),we demonstrate the presence of sexually dimorphic transcriptomes in mouseβcells.Using a high-fat diet-induced T2D mouse model,we identified sex-dependent T2D altered genes,suggesting sex-based differences in the pathological mechanisms of T2D.Furthermore,based on islet transplantation experiments,we found that compared to mice with sexmatched islet transplants,sex-mismatched islet transplants in healthy mice showed down-regulation of genes involved in the longevity regulating pathway ofβcells.Moreover,the diabetic mice with sex-mismatched islet transplants showed impaired glucose tolerance.These data suggest sexual dimorphism in T2D pathogenicity,indicating that sex should be considered when treating T2D.We hope that our findings could provide new insights for the development of precision medicine in T2D.展开更多
基金Supported by grants from the Shandong Province Natural Science Fund Project(No.ZR2013HL004)Colleges and Universities in Shandong Province Science and Technology Plan(No.J10LF57)Binzhou Medical College Students of Science and Technology Innovation Fund Project(No.BY2014DKCX003)
文摘Objective The aim of the study was to investigate whether ethanolic extract of propolis inhibits the growth and induces apoptosis of HL-60 cells. Methods HL-60 cells were treated for 24, 48, 72 h with various concentrations ethanolic extracts of propolis(0, 50, 100, and 200 μg/m L). The proliferation of HL-60 cells was determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay. Subsequently, Hochest 33258 staining and terminal deoxynucleotidyl transferase d UTP nick end labeling(TUNEL) were used to test the apoptosis of HL-60 cells. We observed the expression levels of Bax and Bcl-2 in HL-60 cells by immunohistochemistry. Results MTT assay showed that various concentrations of ethanolic extract of propolis had significant inhibitory effect on HL-60 cell proliferation(P < 0.05). Typical morphologic changes could be observed by fluorescence microscope and TUNEL. By immunohistochemistry, we found the expression level of Bax was up-regulated, whereas that of Bc1-2 was down-regulated(P < 0.05). Conclusion Ethanolic extract of propolis inhibits leukemia cell proliferation and induces apoptosis in vitro. Its mechanism may be related to the regulation of Bax and Bcl-2 expression and up-regulation of Bcl-2/Bax ratio.
基金jointly supported by the National Natural Science Foundation of China [grant numbers 4208810141911540470+3 种基金42075028]the Guangdong Major Project of Basic and Applied Basic Research [grant number 2020B0301030004]the Natural Science Foundation of Guangdong Province of China [grant number 2018A0303130268]the Guangdong Province Key Laboratory for Climate Change and Natural Disaster Studies [grant number2020B1212060025]。
基金This research received funding from the Research and Innovation Initiatives of WHPU(Grant No.2023Y29).
文摘The multifunctional films was prepared by blending chitosan and nano-ZnO with purple tomato anthocyanins or black wolfberry anthocyanins.The properties of films functioned by anthocyanins source and nano-ZnO content were studied.It was found purple tomato anthocyanins showed more significant color change against pH than black wolfberry anthocyanins.The nano-ZnO were widely dispersed in matrix and enhanced the compatibility of anthocyanins with chitosan.However,the anthocyanins source influenced the properties of the films more slightly than nano-ZnO addition.The tensile strength,antioxidant and antibacterial effects of the chitosan films dramatically increased after cooperated by nano-ZnO and anthocyanins,which also enhanced with increase of nano-ZnO content,whereas the elongation at break of the composite films decreased.Especially,the anthocyanin and nano-ZnO promoted the antibacterial activity of films synergistically.Composite films made from black wolfberry anthocyanins exhibited higher mechanical performance than those made from purple tomato anthocyanins but weaker antibacterial effects.The purple tomato anthocyanins/chitosan and nano-ZnO/purple tomato anthocyanins/chitosan films effectively reflected pork spoilage,changing their colors from dark green to brown,indicating the potential for applications in active and intelligent food packaging.
基金financially supported by the National Natural Science Foundation of China(51631001,51672010 and81421004)the National Key R&D Program of China(2017YFA0206301 and 2016YFA0200102)
基金This work was supported by the National Key R&D Program of China(Grant Nos.2016YFA0102200,2017YFA0106500,2018YFA0107102,and 2020YFA0112500 awarded to WL,Grant No.2018YFA0107602 awarded to ZS)Key Project of the Science and Technology Commission of Shanghai Municipality,China(Grant No.19JC1415300 awarded to WL)+2 种基金the National Key R&D Program of China(Grant No.2018YFD0900604 awarded to WS)the National Natural Science Foundation of China(Grant Nos.41676119 and 41476120 awarded to WS)the start-up fund from Ocean University of China(awarded to WS).
文摘Type 2 diabetes(T2D)is characterized by the malfunction of pancreaticβcells.Susceptibility and pathogenesis of T2D can be affected by multiple factors,including sex differences.However,the mechanisms underlying sex differences in T2D susceptibility and pathogenesis remain unclear.Using single-cell RNA sequencing(scRNA-seq),we demonstrate the presence of sexually dimorphic transcriptomes in mouseβcells.Using a high-fat diet-induced T2D mouse model,we identified sex-dependent T2D altered genes,suggesting sex-based differences in the pathological mechanisms of T2D.Furthermore,based on islet transplantation experiments,we found that compared to mice with sexmatched islet transplants,sex-mismatched islet transplants in healthy mice showed down-regulation of genes involved in the longevity regulating pathway ofβcells.Moreover,the diabetic mice with sex-mismatched islet transplants showed impaired glucose tolerance.These data suggest sexual dimorphism in T2D pathogenicity,indicating that sex should be considered when treating T2D.We hope that our findings could provide new insights for the development of precision medicine in T2D.
