The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp ...The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.展开更多
Nanoporous metals have received significant attention as a new class of structural and functional materials.However,the macroscopic brittle fracture under the tensile test is an impediment to their practical applicati...Nanoporous metals have received significant attention as a new class of structural and functional materials.However,the macroscopic brittle fracture under the tensile test is an impediment to their practical applications.Thus,it is of central importance to develop nanoporous materials with low cost and high tensile ductility.Herein,a nanoporous Cu film supported on a pure Cu substrate(NPC@Cu)was fabricated by utilizing a liquid Ga assisted alloying-dealloying strategy,and the thickness of NPC film can be precisely regulated by changing the mass loading of liquid Ga.In-situ X-ray diffraction was performed to further explore the alloying/dealloying mechanisms.The NPC@Cu films show good tensile mechanical properties with a minimum elongation of 13.5%,which can be attributed to the good interface bonding and certain modulus matching between the nanoporous Cu layer and the Cu substrate.Our findings demonstrate that the design of film-substrate structure provides a feasible strategy for enhancing the mechanical properties of nanoporous metals.展开更多
基金Supported by Science and Technology Project of AQSIQ(2013IK277)National Rice Industry System Development Program
文摘The paper aimed to establish a duplex PCR method for simultaneous detection of Pseudomonas savastanoi pv. Phaseolicola （Psp） and Curtobaeterium /accumfadens pv. Flaccumfaciens （Cff）. Based on the argK gene of Psp in GenBank, the primers PSPF1/PSPR2 were designed. The duplex PCR assay was dereloped using the combined primers PSPF1/PSPR2 and CflF1/CffR2, which were specific primers for Cff. The reaction conditions were optimized and specificity md sensitivity of the duplex PCR were tested. The expected DNA fragment was specifically amplified from the genomic DNA of Psp and Cff. Specificity was conirmed in the artificially inoculated soybean samples imparted. Thus, the duplex PCR developed in this study could be used for the simultaneous detection of Psp md Cff from imported soybean.
基金supported by the National Natural Science Foundation of China(Grant No.51871133)the Taishan Scholar Foundation of Shandong Province,the Program of Jinan Science and Technology Bureau(Grant No.2019GXRC001)the Major Projects of Guangdong Education Department for Foundation Research and Applied Research,China(Grant No.2019KZDXM065).
文摘Nanoporous metals have received significant attention as a new class of structural and functional materials.However,the macroscopic brittle fracture under the tensile test is an impediment to their practical applications.Thus,it is of central importance to develop nanoporous materials with low cost and high tensile ductility.Herein,a nanoporous Cu film supported on a pure Cu substrate(NPC@Cu)was fabricated by utilizing a liquid Ga assisted alloying-dealloying strategy,and the thickness of NPC film can be precisely regulated by changing the mass loading of liquid Ga.In-situ X-ray diffraction was performed to further explore the alloying/dealloying mechanisms.The NPC@Cu films show good tensile mechanical properties with a minimum elongation of 13.5%,which can be attributed to the good interface bonding and certain modulus matching between the nanoporous Cu layer and the Cu substrate.Our findings demonstrate that the design of film-substrate structure provides a feasible strategy for enhancing the mechanical properties of nanoporous metals.
