AIM The aim of this study is to investigate the possible correlation between the expression of Nurrl gene and selective cell death of dopamine (DA) neurons. Nurrl ( nuclear receptor-related factor 1 ) is highly expres...AIM The aim of this study is to investigate the possible correlation between the expression of Nurrl gene and selective cell death of dopamine (DA) neurons. Nurrl ( nuclear receptor-related factor 1 ) is highly expressed in mesencephalic DA system and specifically required for development and survival of DArgic neurons, the cells primarily lost and aggressive degenerated in Parkinson’s disease (PD). METHODS Firstly, SK-N-SH cells, which do not express endogenous Nurrl, were transfected with the pBK-RSV-Nurrl plasmid by LipofectAMINE and selected by G418. The expression of Nurrl protein in SK-N-SH/Nurrl cells was determined by immunocytochemistry. The effect of Nurrl gene on cell proliferation was also investigated.展开更多
文摘AIM The aim of this study is to investigate the possible correlation between the expression of Nurrl gene and selective cell death of dopamine (DA) neurons. Nurrl ( nuclear receptor-related factor 1 ) is highly expressed in mesencephalic DA system and specifically required for development and survival of DArgic neurons, the cells primarily lost and aggressive degenerated in Parkinson’s disease (PD). METHODS Firstly, SK-N-SH cells, which do not express endogenous Nurrl, were transfected with the pBK-RSV-Nurrl plasmid by LipofectAMINE and selected by G418. The expression of Nurrl protein in SK-N-SH/Nurrl cells was determined by immunocytochemistry. The effect of Nurrl gene on cell proliferation was also investigated.
