Ambient ozone(O3)exposure may be associated with a reduction of semen quality,yet the potential biological mechanisms remain unclear.We investigated the effects of certain seminal plasma metabolites on mediating the l...Ambient ozone(O3)exposure may be associated with a reduction of semen quality,yet the potential biological mechanisms remain unclear.We investigated the effects of certain seminal plasma metabolites on mediating the links between O3 exposure and the deterioration of semen quality.The untargeted metabolomics analysis was performed on semen samples of 200 Chinese adult men to determine candidate metabolites associated with characteristics of semen quality.Mediation analysis was adopted to examine whether these metabolites modulated the links between O3 exposure and semen quality.We found a significant reduction in sperm concentration by−28.1%(95%CI:−41.7%,−11.3%),and sperm count by−29.2%(95%CI:−43.7%,−11.0%)associated with each 10μg/m3 increase in ambient O3 concentration during the period of sperm development.We delineated 7 metabolites in seminal fluid that substantially mediated the links between O3 exposure and declined semen quality,including myristoleic acid,aspartyl-isoleucine,phenylethyl primeveroside,ACar(18:2),ACar(18:1),FAHFA(22:6/22:3),and LPS(22:5).Among these,myristoleic acid exhibited the most pronounced mediation effects,with its indirect effect of which accounts for 46.4%of the overall association.Our findings suggested that exposure to ozone decreased sperm quality by disrupting fatty acid metabolism,particularly myristoleic acid.展开更多
文摘Ambient ozone(O3)exposure may be associated with a reduction of semen quality,yet the potential biological mechanisms remain unclear.We investigated the effects of certain seminal plasma metabolites on mediating the links between O3 exposure and the deterioration of semen quality.The untargeted metabolomics analysis was performed on semen samples of 200 Chinese adult men to determine candidate metabolites associated with characteristics of semen quality.Mediation analysis was adopted to examine whether these metabolites modulated the links between O3 exposure and semen quality.We found a significant reduction in sperm concentration by−28.1%(95%CI:−41.7%,−11.3%),and sperm count by−29.2%(95%CI:−43.7%,−11.0%)associated with each 10μg/m3 increase in ambient O3 concentration during the period of sperm development.We delineated 7 metabolites in seminal fluid that substantially mediated the links between O3 exposure and declined semen quality,including myristoleic acid,aspartyl-isoleucine,phenylethyl primeveroside,ACar(18:2),ACar(18:1),FAHFA(22:6/22:3),and LPS(22:5).Among these,myristoleic acid exhibited the most pronounced mediation effects,with its indirect effect of which accounts for 46.4%of the overall association.Our findings suggested that exposure to ozone decreased sperm quality by disrupting fatty acid metabolism,particularly myristoleic acid.
