Decellularization is a promising technique to produce natural scaffolds for tissue engineering applications.However,non-crosslinked natural scaffolds disfavor application in cardiovascular surgery due to poor biomecha...Decellularization is a promising technique to produce natural scaffolds for tissue engineering applications.However,non-crosslinked natural scaffolds disfavor application in cardiovascular surgery due to poor biomechanics and rapid degradation.Herein,we proposed a green strategy to crosslink and functionalize acellular scaffolds via the self-assembly of copper@tea polyphenol nanoparticles(Cu@TP NPs),and the resultant nanocomposite acellular scaffolds were named as Cu@TP-dBPs.The crosslinking degree,biomechanics,denaturation temperature and resistance to enzymatic degradation of Cu@TP-dBPs were comparable to those of glutaraldehyde crosslinked decellularized bovine pericardias(Glut-dBPs).Furthermore,Cu@TP-dBPs were biocompatible and had abilities to inhibit bacterial growth and promote the formation of capillary-like networks.Subcutaneous implantation models demonstrated that Cu@TP-dBPs were free of calcification and allowed for host cell infiltration at Day 21.Cardiac patch graft models confirmed that Cu@TP-dBP patches showed improved ingrowth of functional blood vessels and remodeling of extracellular matrix at Day 60.These results suggested that Cu@TP-dBPs not only had comparable biomechanics and biostability to Glut-dBPs,but also had several advantages over Glut-dBPs in terms of anticalcification,remodeling and integration capabilities.Particularly,they were functional patches possessing antibacterial and proangiogenic activities.Thesematerial properties and biological functionsmade Cu@TP-dBPs a promising functional acellular patch for cardiovascular applications.展开更多
基金supported by grant from the National Key Research and Development Program of China(grant number 2016YFC1100900)National Natural Science Foundation of China(grant numbers 81770390,82070402,82170376)Key Research and Development Program of Ningbo(grant number 2018B10092).
文摘Decellularization is a promising technique to produce natural scaffolds for tissue engineering applications.However,non-crosslinked natural scaffolds disfavor application in cardiovascular surgery due to poor biomechanics and rapid degradation.Herein,we proposed a green strategy to crosslink and functionalize acellular scaffolds via the self-assembly of copper@tea polyphenol nanoparticles(Cu@TP NPs),and the resultant nanocomposite acellular scaffolds were named as Cu@TP-dBPs.The crosslinking degree,biomechanics,denaturation temperature and resistance to enzymatic degradation of Cu@TP-dBPs were comparable to those of glutaraldehyde crosslinked decellularized bovine pericardias(Glut-dBPs).Furthermore,Cu@TP-dBPs were biocompatible and had abilities to inhibit bacterial growth and promote the formation of capillary-like networks.Subcutaneous implantation models demonstrated that Cu@TP-dBPs were free of calcification and allowed for host cell infiltration at Day 21.Cardiac patch graft models confirmed that Cu@TP-dBP patches showed improved ingrowth of functional blood vessels and remodeling of extracellular matrix at Day 60.These results suggested that Cu@TP-dBPs not only had comparable biomechanics and biostability to Glut-dBPs,but also had several advantages over Glut-dBPs in terms of anticalcification,remodeling and integration capabilities.Particularly,they were functional patches possessing antibacterial and proangiogenic activities.Thesematerial properties and biological functionsmade Cu@TP-dBPs a promising functional acellular patch for cardiovascular applications.
