The combination of photothermal therapywith chemotherapy has gradually developed into promising cancer therapy.Here,a synergistic photothermal-chemotherapy nanoplatform based on polydopamine(PDA)-coated gold nanoparti...The combination of photothermal therapywith chemotherapy has gradually developed into promising cancer therapy.Here,a synergistic photothermal-chemotherapy nanoplatform based on polydopamine(PDA)-coated gold nanoparticles(AuNPs)were facilely achieved via the in situ polymerization of dopamine(DA)on the surface of AuNPs.This nanoplatform exhibited augmented photothermal conversion efficiency and enhanced colloidal stability in comparison with uncoated PDA shell AuNPs.The i-motif DNA nanostructure was assembled on PDA-coated AuNPs,which could be transformed into a C-quadruplex structure under an acidic environment,showing a characteristic pH response.The PDA shell served as a linker between the AuNPs and the i-motif DNA nanostructure.To enhance the specific cellular uptake,the AS1411 aptamer was introduced to the DNA nanostructure employed as a targeting ligand.In addition,Dox-loaded NPs(DAu@PDA-AS141)showed the pH/photothermal-responsive release of Dox.The photothermal effect of DAu@PDA-AS141 elicited excellent photothermal performance and efficient cancer cell inhibition under 808 nm near-infrared(NIR)irradiation.Overall,these results demonstrate that the DAu@PDA-AS141 nanoplatform shows great potential in synergistic photothermal-chemotherapy.展开更多
Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species...Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species(ROS)by consuming H_(2)O_(2) in TME,improving the inadequate ROS generation problem of PDT.Therefore,synergistic therapy combining PDT and Fenton response-based CDT is a promising ap-proach for cancer treatment.Herein,a metal-polyphenol nanocomposite was deposited with gallic acid grafted hyaluronic acid and Fe^(3+) to contrast a Ce6 nano-delivery system(Ce6@HSF NPs)for melanoma synergistic therapy.Ce6@HSF NPs could be used as a Fenton reagent to induce the·OH production and enhance the PDT effect of Ce6 to a certain extent.After 4 h of cellular uptake,the fluorescence intensity of Ce6 in the Ce6@HSF NPs group was higher than 3 times that in the Ce6 group.The intracellular ROS generation level of the Ce6@HSF NPs(L)group combining CDT and PDT was higher than that of the Ce6 group and Ce6@HSF NPs group.In vitro and in vivo anti-melanoma studies show that Ce6@HSF NPs(L)group exhibited better anti-melanoma than other groups.Together,Ce6@HSF NPs provide a promising synergistic treatment potential for melanoma.展开更多
基金This work was financially supported by National Natural Sciences Foundation of China(31971308 and 82102767)National S&T Major Project(2019ZX09301-147)+1 种基金Sichuan Science and Technology Program(2021YFS0081)Luzhou Science and Technology Plan(2018CDLZ-10).
文摘The combination of photothermal therapywith chemotherapy has gradually developed into promising cancer therapy.Here,a synergistic photothermal-chemotherapy nanoplatform based on polydopamine(PDA)-coated gold nanoparticles(AuNPs)were facilely achieved via the in situ polymerization of dopamine(DA)on the surface of AuNPs.This nanoplatform exhibited augmented photothermal conversion efficiency and enhanced colloidal stability in comparison with uncoated PDA shell AuNPs.The i-motif DNA nanostructure was assembled on PDA-coated AuNPs,which could be transformed into a C-quadruplex structure under an acidic environment,showing a characteristic pH response.The PDA shell served as a linker between the AuNPs and the i-motif DNA nanostructure.To enhance the specific cellular uptake,the AS1411 aptamer was introduced to the DNA nanostructure employed as a targeting ligand.In addition,Dox-loaded NPs(DAu@PDA-AS141)showed the pH/photothermal-responsive release of Dox.The photothermal effect of DAu@PDA-AS141 elicited excellent photothermal performance and efficient cancer cell inhibition under 808 nm near-infrared(NIR)irradiation.Overall,these results demonstrate that the DAu@PDA-AS141 nanoplatform shows great potential in synergistic photothermal-chemotherapy.
基金This work was financially supported by the National Natural Sciences Foundation of China(Nos.31971308,81960769 and U1903211)the National S&T Major Project(No.2019ZX09301-147)+1 种基金the Sichuan Science and Technology Program(No.2022YFS0007)the Luzhou Science and Technology Plan(No.2018CDLZ-10).
文摘Due to the hypoxic state of the tumor microenvironment(TME),photodynamic therapy(PDT)suffers from insufficient ROS production.The metal-polyphenol network-mediated Fenton reaction can generate reac-tive oxygen species(ROS)by consuming H_(2)O_(2) in TME,improving the inadequate ROS generation problem of PDT.Therefore,synergistic therapy combining PDT and Fenton response-based CDT is a promising ap-proach for cancer treatment.Herein,a metal-polyphenol nanocomposite was deposited with gallic acid grafted hyaluronic acid and Fe^(3+) to contrast a Ce6 nano-delivery system(Ce6@HSF NPs)for melanoma synergistic therapy.Ce6@HSF NPs could be used as a Fenton reagent to induce the·OH production and enhance the PDT effect of Ce6 to a certain extent.After 4 h of cellular uptake,the fluorescence intensity of Ce6 in the Ce6@HSF NPs group was higher than 3 times that in the Ce6 group.The intracellular ROS generation level of the Ce6@HSF NPs(L)group combining CDT and PDT was higher than that of the Ce6 group and Ce6@HSF NPs group.In vitro and in vivo anti-melanoma studies show that Ce6@HSF NPs(L)group exhibited better anti-melanoma than other groups.Together,Ce6@HSF NPs provide a promising synergistic treatment potential for melanoma.
