Dear Editor,Targeted genome-editing technology using designed nucleases has been rapidly advancing and catalyzing significant breakthroughs in the life sciences.Custom-designed CRISPR editing experiments can be effici...Dear Editor,Targeted genome-editing technology using designed nucleases has been rapidly advancing and catalyzing significant breakthroughs in the life sciences.Custom-designed CRISPR editing experiments can be efficiently evaluated through nextgeneration sequencing(NGS).Nevertheless,NGS data analysis currently lacks a user-friendly pipeline capable of automatically calculating mutations and evaluating editing efficiency in bulk,as well as providing a more comprehensive visualization of results.展开更多
基金supported by the National Natural Science Foundation of China (grant no.32270585)the Key R&D Program of Jiangsu Province (Modern Agriculture) (BE2022335)+2 种基金the Project of Zhongshan Biological Breeding Laboratory (BM2022008-02)the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)to T.Z.,the National Natural Science Foundation of China (award no.32270433)to Y.Z.
文摘Dear Editor,Targeted genome-editing technology using designed nucleases has been rapidly advancing and catalyzing significant breakthroughs in the life sciences.Custom-designed CRISPR editing experiments can be efficiently evaluated through nextgeneration sequencing(NGS).Nevertheless,NGS data analysis currently lacks a user-friendly pipeline capable of automatically calculating mutations and evaluating editing efficiency in bulk,as well as providing a more comprehensive visualization of results.
