Because of highly intensive farming practices, crop plants could suffer relatively long terms of ammonium(NH4+) excess stress introduced by overdose application of nitrogen fertilizers. However, the lack of sufficient...Because of highly intensive farming practices, crop plants could suffer relatively long terms of ammonium(NH4+) excess stress introduced by overdose application of nitrogen fertilizers. However, the lack of sufficient understanding of plant responses to NH4+excess stress impairs the detection of effective solutions to this problem. The present work examined the biological influences of over-supplied NH4+in Arabidopsis thaliana using two mutant lines each with an ammonium transporter(AMT) gene(AtAMT1;1 or AtAMT1;3) knocked out. Our results indicated that lacking one of the major components of root NH4+-absorbing systems significantly alleviated the toxicity effects on Arabidopsis plants by reducing the accumulation of free NH4+, suggesting that persistent absorption of NH4+through AMT was the main cause of excessive accumulation of free NH4+in the plants. Shading treatment led to a reduced transpirational driving force and thereby constrained the accumulation of toxic NH4+in the plants, finally resulting in higher NH4+-promoted growth in the wild type(WT). Under the shading treatment, the amt1;1 and amt1;3 mutant plants acquired insufficient NH4+and showed reduced growth when compared with the WT. Furthermore, the foliar application of sucrose notably alleviated the inhibitory effects on plant growth in the WT but had no effect on either the amt1;1 or amt1;3 mutant plants, indicating that carbon scarcity associated with NH4+excess is probably a major cause of NH4+toxicity in plants. Accordingly,increasing carbon source could be a potentially effective approach that alleviates the inhibition caused by NH4+excess and increases nitrogen use efficiency under NH4+over-supply.展开更多
Although studies have compared the relative severity of Omicron and Delta variants by assessing the relative risks,there are still gaps in the knowledge of the potential COVID-19 burden these variations may cause.And ...Although studies have compared the relative severity of Omicron and Delta variants by assessing the relative risks,there are still gaps in the knowledge of the potential COVID-19 burden these variations may cause.And the contact patterns in Fujian Province,China,have not been described.We identified 8969 transmission pairs in Fujian,China,by analyzing a contact-tracing database that recorded a SARS-CoV-2 outbreak in September 2021.We estimated the waning vaccine effectiveness against Delta variant infection,contact patterns,and epidemiology distributions,then simulated potential outbreaks of Delta and Omicron variants using a multi-group mathematical model.For instance,in the contact setting without stringent lockdowns,we estimated that in a potential Omicron wave,only 4.7%of infections would occur in Fujian Province among individuals aged>60 years.In comparison,58.75%of the death toll would occur in unvaccinated individuals aged>60 years.Compared with no strict lockdowns,combining school or factory closure alone reduced cumulative deaths of Delta and Omicron by 28.5%and 6.1%,respectively.In conclusion,this study validates the need for continuous mass immunization,especially among elderly aged over 60 years old.And it confirms that the effect of lockdowns alone in reducing infections or deaths is minimal.However,these measurements will still contribute to lowering peak daily incidence and delaying the epidemic,easing the healthcare system's burden.展开更多
In eukaryotic cells, chromatin transformation from euchromatin into heterochromatin as a means of controlling gene expression and replication has been known as the ‘‘accessibility hypothesis' '. The interpla...In eukaryotic cells, chromatin transformation from euchromatin into heterochromatin as a means of controlling gene expression and replication has been known as the ‘‘accessibility hypothesis' '. The interplay of epigenetic changes including histone modifications, DNA methylation, RNA interference(RNAi) and other func- tional epigenetic components are intricate. It is believed that these changes are well-programmed, inherited and can be modified by environmental contaminant stressors. Environmentally-driven epigenetic alterations during development, e.g. embryonic, foetal or neonatal stage, may influence disease susceptibility in adulthood. Therefore, understanding how epigenome modifications develop in response to environmental chemicals and, how epigenetic-xenobiotic interactions influence human health will shed new insights into gene-environment interactions in the epidemiology of several diseases including cancer. In this review, we consider studies of chemical modifiers including nutritional and xenobiotic effects on epigenetic components in vitro or in vivo. By examining the most-studied epigenome modifications and how their respective roles are interlinked, we highlight the central role of xenbiotic- modified epigenetic mechanisms. A major requirement will be to study and understand effects following environmentally-relevant exposures. We suggest that the study of epigenetic toxicology will open up new opportunities to devise strategies for the prevention or treatment of at-risk populations.展开更多
Introduction:Vaccination booster shots are completely necessary for controlling breakthrough infections of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in China.The study aims to estimate effectiveness o...Introduction:Vaccination booster shots are completely necessary for controlling breakthrough infections of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in China.The study aims to estimate effectiveness of booster vaccines for high-risk populations(HRPs).Methods:A vaccinated Susceptible-Exposed-Symptomatic-Asymptomatic-Recovered/Removed(SEIAR)model was developed to simulate scenarios of effective reproduction number(Reff)from 4 to 6.Total number of infectious and asymptomatic cases were used to evaluated vaccination effectiveness.Results:Our model showed that we could not prevent outbreaks when covering 80%of HRPs with booster unless Reff=4.0 or the booster vaccine had efficacy against infectivity and susceptibility of more than 90%.The results were consistent when the outcome index was confirmed cases or asymptomatic cases.Conclusions:An ideal coronavirus disease 2019(COVID-19)booster vaccination strategy for HRPs would be expected to reach the initial goal to control the transmission of the Delta variant in China.Accordingly,the recommendation for the COVID-19 booster vaccine should be implemented in HRPs who are already vaccinated and could prevent transmission to other groups.展开更多
Objective:In China,the burden of shigellosis is unevenly distributed,notably across various ages and geographical areas.Shigellosis temporal trends appear to be seasonal.We should clarify seasonal warnings and regiona...Objective:In China,the burden of shigellosis is unevenly distributed,notably across various ages and geographical areas.Shigellosis temporal trends appear to be seasonal.We should clarify seasonal warnings and regional transmission patterns.Method:This study adopted a Logistic model to assess the seasonality and a dynamics model to compare the transmission in different areas.The next-generation matrix was used to calculate the effective reproduction number(Reff)to quantify the transmissibility.Results:In China,the rate of shigellosis fell from 35.12 cases per 100,000 people in 2005 to 7.85 cases per 100,000 people in 2017,peaking in June and August.After simulation by the Logistic model,the‘peak time’is mainly concentrated from mid-June to mid-July.China's‘early warning time’is primarily focused on from April to May.We predict the‘peak time’of shigellosis is the 6.30th month and the‘early warning time’is 3.87th month in 2021.According to the dynamics model results,the water/food transfer pathway has been mostly blocked off.The transmissibility of different regions varies greatly,such as the mean Reff of Longde County(3.76)is higher than Xiamen City(3.15),higher than Chuxiong City(2.52),and higher than Yichang City(1.70).Conclusion:The‘early warning time’for shigellosis in China is from April to May every year,and it may continue to advance in the future,such as the early warning time in 2021 is in mid-March.Furthermore,we should focus on preventing and controlling the personto-person route of shigellosis and stratified deploy prevention and control measures according to the regional transmission.展开更多
Introduction:The coronavirus disease 2019(COVID-19)pandemic recently affected Taiwan,China.This study aimed to calculate the transmissibility of COVID-19 to predict trends and evaluate the effects of interventions.Met...Introduction:The coronavirus disease 2019(COVID-19)pandemic recently affected Taiwan,China.This study aimed to calculate the transmissibility of COVID-19 to predict trends and evaluate the effects of interventions.Methods:The data of reported COVID-19 cases was collected from April 20 to May 26,2021,which included daily reported data(Scenario I)and reported data after adjustment(Scenario II).A susceptibleexposed-symptomatic-asymptomatic-recovered model was developed to fit the data.The effective reproductive number(Reff)was used to estimate the transmissibility of COVID-19.Results:A total of 4,854 cases were collected for the modelling.In Scenario I,the intervention has already taken some effects from May 17 to May 26(the Reff reduced to 2.1).When the Reff was set as 0.1,the epidemic was projected to end on July 4,and a total of 1,997 cases and 855 asymptomatic individuals would have been reported.In Scenario II,the interventions were projected as having been effective from May 24 to May 26(the Reff reduced to 0.4).When the Reff was set as 0.1,the epidemic was projected to end on July 1,and a total of 1,482 cases and 635 asymptomatic individuals would have been reported.Conclusion:The epidemic of COVID-19 was projected to end after at least one month,even if the most effective interventions were applied in Taiwan,China.Although there were some positive effects of intervention in Taiwan,China.展开更多
Many genes associated with reproduction show rapid evolution across diverse animal groups, a result commonly due to adaptive evolution driven by positive selection (Swanson and Vacquier, 2002). Different theories ha...Many genes associated with reproduction show rapid evolution across diverse animal groups, a result commonly due to adaptive evolution driven by positive selection (Swanson and Vacquier, 2002). Different theories have been proposed to explain the elevated rates of evolution (Swanson and Vacquier, 2002), including sperm competition, where sperm compete to fertilize eggs leading to the proteins in the sper- matozoa adaptively evolving to increase their ability to fertilize eggs; sexual conflict, where the egg experiences a loss of fitness when sperm are too abundant; sexual selection, where eggs bind sperm carrying adaptive alleles (Palumbi, 1999); and cryptic female choice (reviewed in Swanson and Vacquier, 2002).展开更多
Objectives:Computing the basic reproduction number(R0)in deterministic dynamical models is a hot topic and is frequently demanded by researchers in public health.The nextgeneration methods(NGM)are widely used for such...Objectives:Computing the basic reproduction number(R0)in deterministic dynamical models is a hot topic and is frequently demanded by researchers in public health.The nextgeneration methods(NGM)are widely used for such computation,however,the results of NGM are usually not to be the true R0 but only a threshold quantity with little interpretation.In this paper,a definition-based method(DBM)is proposed to solve such a problem.Methods:Start with the definition of R0,consider different states that one infected individual may develop into,and take expectations.A comparison with NGM has proceeded.Numerical verification is performed using parameters fitted by data of COVID-19 in Hunan Province.Results:DBM and NGM give identical expressions for single-host models with single-group and interactive Rij of single-host models with multi-groups,while difference arises for models partitioned into subgroups.Numerical verification showed the consistencies and differences between DBM and NGM,which supports the conclusion that R0 derived by DBM with true epidemiological interpretations are better.Conclusions:DBM is more suitable for single-host models,especially for models partitioned into subgroups.However,for multi-host dynamic models where the true R0 is failed to define,we may turn to the NGM for the threshold R0.展开更多
基金This work was supported by the National Key Research&Development Program of China(Nos.2017YFD0200100 and 2017YFD0200103)the Strategic Priority Research Program of Chinese Academy of Sciences(CAS)(No.XDB15030202)the Project of Priority and Key Areas of the Institute of Soil Science,CAS(No.ISSASIP1609).
文摘Because of highly intensive farming practices, crop plants could suffer relatively long terms of ammonium(NH4+) excess stress introduced by overdose application of nitrogen fertilizers. However, the lack of sufficient understanding of plant responses to NH4+excess stress impairs the detection of effective solutions to this problem. The present work examined the biological influences of over-supplied NH4+in Arabidopsis thaliana using two mutant lines each with an ammonium transporter(AMT) gene(AtAMT1;1 or AtAMT1;3) knocked out. Our results indicated that lacking one of the major components of root NH4+-absorbing systems significantly alleviated the toxicity effects on Arabidopsis plants by reducing the accumulation of free NH4+, suggesting that persistent absorption of NH4+through AMT was the main cause of excessive accumulation of free NH4+in the plants. Shading treatment led to a reduced transpirational driving force and thereby constrained the accumulation of toxic NH4+in the plants, finally resulting in higher NH4+-promoted growth in the wild type(WT). Under the shading treatment, the amt1;1 and amt1;3 mutant plants acquired insufficient NH4+and showed reduced growth when compared with the WT. Furthermore, the foliar application of sucrose notably alleviated the inhibitory effects on plant growth in the WT but had no effect on either the amt1;1 or amt1;3 mutant plants, indicating that carbon scarcity associated with NH4+excess is probably a major cause of NH4+toxicity in plants. Accordingly,increasing carbon source could be a potentially effective approach that alleviates the inhibition caused by NH4+excess and increases nitrogen use efficiency under NH4+over-supply.
基金supported by the Bill&Melinda Gates Foundation(INV-005834),Natural Science Foundation of Fujian Province(NO.2021J01353,NO.2020J01094)National Science and Technology Major Project of the Ministry of Science and Technology of China(NO.2018ZX10734402-007)+1 种基金Research on accurate prediction and timely response system for out-breaks of new infectious diseases(SRPG2200702)Fundamental Research Funds for the Central Universities(No.20720230001).
文摘Although studies have compared the relative severity of Omicron and Delta variants by assessing the relative risks,there are still gaps in the knowledge of the potential COVID-19 burden these variations may cause.And the contact patterns in Fujian Province,China,have not been described.We identified 8969 transmission pairs in Fujian,China,by analyzing a contact-tracing database that recorded a SARS-CoV-2 outbreak in September 2021.We estimated the waning vaccine effectiveness against Delta variant infection,contact patterns,and epidemiology distributions,then simulated potential outbreaks of Delta and Omicron variants using a multi-group mathematical model.For instance,in the contact setting without stringent lockdowns,we estimated that in a potential Omicron wave,only 4.7%of infections would occur in Fujian Province among individuals aged>60 years.In comparison,58.75%of the death toll would occur in unvaccinated individuals aged>60 years.Compared with no strict lockdowns,combining school or factory closure alone reduced cumulative deaths of Delta and Omicron by 28.5%and 6.1%,respectively.In conclusion,this study validates the need for continuous mass immunization,especially among elderly aged over 60 years old.And it confirms that the effect of lockdowns alone in reducing infections or deaths is minimal.However,these measurements will still contribute to lowering peak daily incidence and delaying the epidemic,easing the healthcare system's burden.
基金supported by Hundred Talent Program of Chinese Academy of Sciences for 2010 on Human Exposure to Environmental Pollutant and Health Effect,National Natural Science Foundation of China(21177123)Xiamen Science and Technology Fund(3502Z20122003)
文摘In eukaryotic cells, chromatin transformation from euchromatin into heterochromatin as a means of controlling gene expression and replication has been known as the ‘‘accessibility hypothesis' '. The interplay of epigenetic changes including histone modifications, DNA methylation, RNA interference(RNAi) and other func- tional epigenetic components are intricate. It is believed that these changes are well-programmed, inherited and can be modified by environmental contaminant stressors. Environmentally-driven epigenetic alterations during development, e.g. embryonic, foetal or neonatal stage, may influence disease susceptibility in adulthood. Therefore, understanding how epigenome modifications develop in response to environmental chemicals and, how epigenetic-xenobiotic interactions influence human health will shed new insights into gene-environment interactions in the epidemiology of several diseases including cancer. In this review, we consider studies of chemical modifiers including nutritional and xenobiotic effects on epigenetic components in vitro or in vivo. By examining the most-studied epigenome modifications and how their respective roles are interlinked, we highlight the central role of xenbiotic- modified epigenetic mechanisms. A major requirement will be to study and understand effects following environmentally-relevant exposures. We suggest that the study of epigenetic toxicology will open up new opportunities to devise strategies for the prevention or treatment of at-risk populations.
文摘Introduction:Vaccination booster shots are completely necessary for controlling breakthrough infections of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in China.The study aims to estimate effectiveness of booster vaccines for high-risk populations(HRPs).Methods:A vaccinated Susceptible-Exposed-Symptomatic-Asymptomatic-Recovered/Removed(SEIAR)model was developed to simulate scenarios of effective reproduction number(Reff)from 4 to 6.Total number of infectious and asymptomatic cases were used to evaluated vaccination effectiveness.Results:Our model showed that we could not prevent outbreaks when covering 80%of HRPs with booster unless Reff=4.0 or the booster vaccine had efficacy against infectivity and susceptibility of more than 90%.The results were consistent when the outcome index was confirmed cases or asymptomatic cases.Conclusions:An ideal coronavirus disease 2019(COVID-19)booster vaccination strategy for HRPs would be expected to reach the initial goal to control the transmission of the Delta variant in China.Accordingly,the recommendation for the COVID-19 booster vaccine should be implemented in HRPs who are already vaccinated and could prevent transmission to other groups.
基金supported by the Bill&Melinda Gates Foundation(INV-005834)the Science and Technology Program of Fujian Province(No:2020Y0002)the Xiamen New Coronavirus Prevention and Control Emergency Tackling Special Topic Program(No:3502Z2020YJ03).
文摘Objective:In China,the burden of shigellosis is unevenly distributed,notably across various ages and geographical areas.Shigellosis temporal trends appear to be seasonal.We should clarify seasonal warnings and regional transmission patterns.Method:This study adopted a Logistic model to assess the seasonality and a dynamics model to compare the transmission in different areas.The next-generation matrix was used to calculate the effective reproduction number(Reff)to quantify the transmissibility.Results:In China,the rate of shigellosis fell from 35.12 cases per 100,000 people in 2005 to 7.85 cases per 100,000 people in 2017,peaking in June and August.After simulation by the Logistic model,the‘peak time’is mainly concentrated from mid-June to mid-July.China's‘early warning time’is primarily focused on from April to May.We predict the‘peak time’of shigellosis is the 6.30th month and the‘early warning time’is 3.87th month in 2021.According to the dynamics model results,the water/food transfer pathway has been mostly blocked off.The transmissibility of different regions varies greatly,such as the mean Reff of Longde County(3.76)is higher than Xiamen City(3.15),higher than Chuxiong City(2.52),and higher than Yichang City(1.70).Conclusion:The‘early warning time’for shigellosis in China is from April to May every year,and it may continue to advance in the future,such as the early warning time in 2021 is in mid-March.Furthermore,we should focus on preventing and controlling the personto-person route of shigellosis and stratified deploy prevention and control measures according to the regional transmission.
基金Bill&Melinda Gates Foundation(INV-005834)the Science and Technology Program of Fujian Province(No:2020Y0002)Provincial Natural Science Foundation of Fujian Province(No:2020J01094).
文摘Introduction:The coronavirus disease 2019(COVID-19)pandemic recently affected Taiwan,China.This study aimed to calculate the transmissibility of COVID-19 to predict trends and evaluate the effects of interventions.Methods:The data of reported COVID-19 cases was collected from April 20 to May 26,2021,which included daily reported data(Scenario I)and reported data after adjustment(Scenario II).A susceptibleexposed-symptomatic-asymptomatic-recovered model was developed to fit the data.The effective reproductive number(Reff)was used to estimate the transmissibility of COVID-19.Results:A total of 4,854 cases were collected for the modelling.In Scenario I,the intervention has already taken some effects from May 17 to May 26(the Reff reduced to 2.1).When the Reff was set as 0.1,the epidemic was projected to end on July 4,and a total of 1,997 cases and 855 asymptomatic individuals would have been reported.In Scenario II,the interventions were projected as having been effective from May 24 to May 26(the Reff reduced to 0.4).When the Reff was set as 0.1,the epidemic was projected to end on July 1,and a total of 1,482 cases and 635 asymptomatic individuals would have been reported.Conclusion:The epidemic of COVID-19 was projected to end after at least one month,even if the most effective interventions were applied in Taiwan,China.Although there were some positive effects of intervention in Taiwan,China.
基金supported by the grant from the National Natural Science Foundation of China(No.31061160189)
文摘Many genes associated with reproduction show rapid evolution across diverse animal groups, a result commonly due to adaptive evolution driven by positive selection (Swanson and Vacquier, 2002). Different theories have been proposed to explain the elevated rates of evolution (Swanson and Vacquier, 2002), including sperm competition, where sperm compete to fertilize eggs leading to the proteins in the sper- matozoa adaptively evolving to increase their ability to fertilize eggs; sexual conflict, where the egg experiences a loss of fitness when sperm are too abundant; sexual selection, where eggs bind sperm carrying adaptive alleles (Palumbi, 1999); and cryptic female choice (reviewed in Swanson and Vacquier, 2002).
基金supported by the Bill&Melinda Gates Foundation(INV-005834)The National Key Research and Development Program of China(2021YFC2301604)the research project on education and teaching reform of undergraduate universities of Fujian Province,China(FBJG20210260).
文摘Objectives:Computing the basic reproduction number(R0)in deterministic dynamical models is a hot topic and is frequently demanded by researchers in public health.The nextgeneration methods(NGM)are widely used for such computation,however,the results of NGM are usually not to be the true R0 but only a threshold quantity with little interpretation.In this paper,a definition-based method(DBM)is proposed to solve such a problem.Methods:Start with the definition of R0,consider different states that one infected individual may develop into,and take expectations.A comparison with NGM has proceeded.Numerical verification is performed using parameters fitted by data of COVID-19 in Hunan Province.Results:DBM and NGM give identical expressions for single-host models with single-group and interactive Rij of single-host models with multi-groups,while difference arises for models partitioned into subgroups.Numerical verification showed the consistencies and differences between DBM and NGM,which supports the conclusion that R0 derived by DBM with true epidemiological interpretations are better.Conclusions:DBM is more suitable for single-host models,especially for models partitioned into subgroups.However,for multi-host dynamic models where the true R0 is failed to define,we may turn to the NGM for the threshold R0.