Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained seve...Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes.Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage.In this study,we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring.Consequently,we obtained the late flowering Chinese cabbage-cabbage translocation line‘AT7–4’in which the flowering time was later than that of‘85–1’by about 7 days under 4-week vernalization.Based on previous studies of the genomes of Chinese cabbage and cabbage,we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line‘AT7–4’and Chinese cabbage‘14–36’.Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed.This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.展开更多
Fleshy fruit shape is an important external quality trait influencing the usage of fruits and consumer preference.Thus,modification of fruit shape has become one of the major objectives for crop improvement.However,th...Fleshy fruit shape is an important external quality trait influencing the usage of fruits and consumer preference.Thus,modification of fruit shape has become one of the major objectives for crop improvement.However,the underlying mechanisms of fruit shape regulation are poorly understood.In this review we summarize recent progress in the genetic basis of fleshy fruit shape regulation using tomato,cucumber,and peach as examples.Comparative analyses suggest that the OFP-TRM(OVATE Family Protein-TONNEAU1 Recruiting Motif)and IQD(IQ67 domain)pathways are probably conserved in regulating fruit shape by primarily modulating cell division patterns across fleshy fruit species.Interestingly,cucumber homologs of FRUITFULL(FUL1),CRABS CLAW(CRC)and 1-aminocyclopropane-1-carboxylate synthase 2(ACS2)were found to regulate fruit elongation.We also outline the recent progress in fruit shape regulation mediated by OFP-TRM and IQD pathways in Arabidopsis and rice,and propose that the OFP-TRM pathway and IQD pathway coordinate regulate fruit shape through integration of phytohormones,including brassinosteroids,gibberellic acids,and auxin,and microtubule organization.In addition,functional redundancy and divergence of the members of each of the OFP,TRM,and IQD families are also shown.This review provides a general overview of current knowledge in fruit shape regulation and discusses the possible mechanisms that need to be addressed in future studies.展开更多
The genus Brassica contains a rich diversity of species and morphological types,including leaf,root,and oil crops,all of which show substantial phenotypic variation.Both Chinese cabbage and cabbage are typical leaf-ty...The genus Brassica contains a rich diversity of species and morphological types,including leaf,root,and oil crops,all of which show substantial phenotypic variation.Both Chinese cabbage and cabbage are typical leaf-type crops with normal roots.We created translocation lines based on interspecific crosses between Chinese cabbage and cabbage and identified qdh225,which exhibited a swollen-root phenotype.The swollen root of qdh225 contained a large number of granular substances,and the formation of its irregular morphological tissue was caused by a thickening of the phloem.Transcriptomic and metabolomic data suggested that differential expression of genes encoding nine types of enzymes involved in starch and sucrose metabolism caused changes in starch synthesis and degradation in the swollen root.These genes jointly regulated sucrose and starch levels,leading to significant enrichment of starch and soluble proteins in the swollen root and a reduction in the content of soluble sugars such as d-glucose and trehalose 6-phosphate.A significant increase in auxin(IAA)and abscisic acid(ABA)contents and a decrease in gibberellin(GA)content in the swollen root likely promoted the differential expression of genes associated with hormone signal transduction,thereby regulating the development of the swollen root.Taken together,our data suggest that accumulation of IAA and ABA and reduction in GA promote swollen root formation by regulating hormone-mediated signaling,leading to a thickening of phloem,root enlargement,and substantial accumulation of starch and soluble proteins.The latter provide materials,energy,and nutrient sources for the development of swollen roots.展开更多
Objective: To evaluate the diagnostic value and safety of CT and ultrasound-guided PTNB for peripheral type of occupying lung lesions by meta-analysis and clinical study. Methods: 1) The target data of randomized cont...Objective: To evaluate the diagnostic value and safety of CT and ultrasound-guided PTNB for peripheral type of occupying lung lesions by meta-analysis and clinical study. Methods: 1) The target data of randomized controlled trials of CT and ultrasound-guided PTNB for peripheral type of occupying lung lesions were extracted by computer search of foreign PubMed, Embase, the Cochrane Library, Web of Science databases, since the establishment of the database. Cochrane quality assessment criteria were used for evaluation. Statistical analysis was performed using Review Manager 5.3 software. 2) To retrospectively study the diagnosis rate and complication rate of patients, undergoing CT or ultrasound-guided PTNB in the First Affiliated Hospital of Dali University from January 2015 to December 2020. Results: Meta-analysis included 7 papers with a total of 1177 patients including 502 patients in the ultrasound group and 675 patients in the CT group. Meta-analysis results showed that there was no difference in the diagnosis rate of PTNB guided by ultrasound and CT. The incidence of postoperative complications was higher in the CT group than in the ultrasound group. The incidence of postoperative pneumothorax was higher in the CT group than in the ultrasound group, and there was no difference in the incidence of postoperative bleeding. 3) Clinical study results show that the puncture success rate was 100% in both of the ultrasound and CT groups, the pathological diagnosis rate was 85.48% in the ultrasound group and 91.67% in the CT group, and there was no difference in the overall complication rate between the two puncture groups. Conclusion: Either ultrasound or CT-guided PTNB is a safe and effective clinical diagnostic method for the diagnosis of peripheral pulmonary occupations.展开更多
Radar is an electronic device that uses radio waves to determine the range, angle, or velocity of objects. Real-time signal and information processor is an important module for real-time positioning, imaging, detectio...Radar is an electronic device that uses radio waves to determine the range, angle, or velocity of objects. Real-time signal and information processor is an important module for real-time positioning, imaging, detection and recognition of targets. With the development of ultra-wideband technology, synthetic aperture technology, signal and information processing technology, the radar coverage, detection accuracy and resolution have been greatly improved, especially in terms of one-dimensional(1D) high-resolution radar detection, tracking, recognition, and two-dimensional(2D) synthetic aperture radar imaging technology. Meanwhile, for the application of radar detection and remote sensing with high resolution and wide swath, the amount of data has been greatly increased. Therefore, the radar is required to have low-latency and real-time processing capability under the constraints of size, weight and power consumption. This paper systematically introduces the new technology of high resolution radar and real-time signal and information processing. The key problems and solutions are discussed, including the detection and tracking of 1D high-resolution radar, the accurate signal modeling and wide-swath imaging for geosynchronous orbit synthetic aperture radar, and real-time signal and information processing architecture and efficient algorithms. Finally, the latest research progress and representative results are presented, and the development trends are prospected.展开更多
Pectobacterium carotovorum ssp.carotovorum(Pcc)is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage.In this study,plants harboring the resistant mutant sr gene,which confers resistance a...Pectobacterium carotovorum ssp.carotovorum(Pcc)is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage.In this study,plants harboring the resistant mutant sr gene,which confers resistance against Pcc,were screened from an 800 M2 population mutated by ethyl methane sulfonate(EMS)and scored in vitro and in vivo for lesion size.The transcript profiles showed~512 differentially expressed genes(DEGs)between sr and WT plants occurring between 6 and 12 h postinoculation(hpi),which corresponded to the important defense regulation period(resistance)to Pcc in Chinese cabbage.The downstream defense genes(CPK,CML,RBOH MPK3,and MPK4)of pathogen pattern-triggered immunity(PTI)were strongly activated during infection at 12 hpi in resistant mutant sr;PTI appears to be central to plant defense against Pcc via recognition by three putative pattern recognition receptors(PRRs;BrLYM1-BrCERK1,BrBKK1/SERK4-PEPR1,BrWAKs).Pcc triggered the upregulation of the jasmonic acid(JA)and ethylene(ET)biosynthesis genes in mutant sr,but auxins and other hormones may have affected some negative signals.Endogenous hormones(auxins,JAs,and SA),as well as exogenous auxins(MEJA and BTH),were also verified as functioning in the immune system.Concurrently,the expression of glucosinolate and lignin biosynthesis genes was increased at 12 hpi in resistant mutant sr,and the accumulation of glucosinolate and lignin also indicated that these genes have a functional defensive role against Pcc.Our study provides valuable information and elucidates the resistance mechanism of Chinese cabbage against Pcc infection.展开更多
The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the head...The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the heading leaves curve inward and overlap at the top,is the shape preferred by consumers.To understand the genetic regulation of HTS,we generated a large segregating F 2 population from a cross between pak choi and Chinese cabbage,with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves.HTS was correlated with plant height,outer/rosette leaf length,and petiole length.A high-density genetic map was constructed.Quantitative trait locus(QTL)analysis resulted in the identification of 22 QTLs for leafy head-related traits,which included five HTS QTLs.Bulked segregant analysis(BSA)was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms.Interestingly,the HTS QTLs colocalized with QTLs for plant height,outer/rosette leaf,and petiole length,consistent with the observed phenotypic correlations.Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait.展开更多
The antipode of a Yetter-Drinfeld Hopf algebra is an anti-algebra and anti-coalgebra map is proved. It is also proved that the tensor algebra of Yetter-Drinfeld Hopf module is a Yetter-Drinfeld Hopf algebra.
In this paper, the fundamental theorem of Yetter-Drinfeld Hopf module is proved. As applications, the freedom of tensor and twisted tensor of two Yetter-Drinfeld Hopf algebras is given. Let A be a Yetter-Drinfeld Hopf...In this paper, the fundamental theorem of Yetter-Drinfeld Hopf module is proved. As applications, the freedom of tensor and twisted tensor of two Yetter-Drinfeld Hopf algebras is given. Let A be a Yetter-Drinfeld Hopf algebra. It is proved that the category of A-bimodule is equivalent to the category of ? -twisted module.展开更多
With the complementation of whole-genome sequencing of Chinese cabbage,it is necessary to develop genome-wide Simple Sequence Repeat(SSR)markers and analyse their characteristics,which will bring a revolution in the m...With the complementation of whole-genome sequencing of Chinese cabbage,it is necessary to develop genome-wide Simple Sequence Repeat(SSR)markers and analyse their characteristics,which will bring a revolution in the molecular marker-assisted breeding of Chinese cabbage.In this study distribution and characteristics of SSR loci in the genome of Chinese cabbage'Chiifu'was analyzed.Finally,a total of 173,892 SSR markers that occurred in 10 chromosomes were identified,and Perfect Repeat Motifs(P type)which is a main form of SSR loci with a mean distance of 1.89 kb/SSR was identified.Among them,mono-,di-,and tri-nucleotide repeat motifs were equally distributed across chromosomes compared with tetra-,penta-,and hexa-nucleotide repeat motifs.The largest proportion of SSR loci consisted of mono-and dinucleotide repeat motifs,which accounted for 93.04%of the total SSR loci.A/T and AT/TA were the most abundant motifs,accounting for 97.61%and 64.05%of the mono-and dinucleotide repeats,respectively.Furthermore,200 SSR markers were used for the amplification of 12 Chinese cabbage germplasm,in which 30 were highly polymorphic.These SSR markers were further used for the association analyses of flowering time of 190 Chinese cabbage accessions.The results revealed that 9 and 10 alleles were associated with bolting and flowering time,respectivley.The results show that development of SSR markers is feasible and useful in marker assisted selection of Chinese cabbage.展开更多
Due to strong synergistic effect of the elements,a series of XEuMnO_(x) ternary oxides(X=Ce,Ni,Co,Sb,Sn,Mo) were synthesized by one-pot co-precipitation method,and composite components were identified and optimized to...Due to strong synergistic effect of the elements,a series of XEuMnO_(x) ternary oxides(X=Ce,Ni,Co,Sb,Sn,Mo) were synthesized by one-pot co-precipitation method,and composite components were identified and optimized to maintain high activity and superior SO_(2)and H_(2)O endurance in selective catalytic reduction of NO_(x)with NH_(3)(NH_(3)-SCR).NO_(x)conversion of CeEuMnO_(x) ternary oxide catalysts attains more than 90% at 100-250℃,and finally achieves 74%under existence of 50×10^(-6)SO_(2) and 10 vol% H_(2)O at 230℃.The facile electron transfer through redox cycle of Mn^(3+)+Ce^(4+)■Mn4++Ce^(3+) and enhanced oxygen mobility can promote formation of more Mn species in high oxidation state and chemisorbed oxygen,accelerating oxidation of NO and the adsorbed NO_(2) formed can facilitate"fast SCR"reaction to improve low-temperature activity.In situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS)study reveals that addition of Ce to EuMnO_(x)catalyst boosts adsorption of NH_(3)and NO_(x)species.NH_(3)species are activated as crucial intermediate(NH_(2))to promote NH_(3)-SCR reaction.This research provides a novel material for practical deNO_(x)application of stationary source combustion flue gas in the future.展开更多
In this paper,we describe multiplicative derivations on the set of all rank-s matrices of Mn(K)over a field K with a relatively small integer s.Concretely,for fixed integers n,s satisfying 1≤s≤n/2 and n≥2,we prove ...In this paper,we describe multiplicative derivations on the set of all rank-s matrices of Mn(K)over a field K with a relatively small integer s.Concretely,for fixed integers n,s satisfying 1≤s≤n/2 and n≥2,we prove that if a map d:Mn(K)→Mn(K)satisfiesδ(xy)=δ(x)y+xδ(y)for any two rank-s matrices,y∈Mn(K),then there exists a derivation D of Mn(K)such thatδ(x)=D(x)for each rank-k matrix a E Mn(K)with 0≤k≤s.As an application,we prove that a multiplicative derivation on a special subset of Mn(K)must be a derivation.展开更多
OBJECTIVE: To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. METHODS: MI/R ...OBJECTIVE: To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. METHODS: MI/R injury was induced by the occlusion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and malondialdehyde (MDA). Blood samples were col- lected to determine serum levels of creatine kinase-MB (CK-MB), cardiac troponin (cTnI) and lactate dehydrogenase (LDH). Furthermore, simulatedischemia/reperfusion (SI/R) injury in vitro was established by oxygen and glucose deprivation (OGD) for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured supernatant and the levels of intracellular reactive oxygen species (ROS), SOD and MDA in cardiomyocytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS: MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnI and LDH. Additionally, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION: MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties.展开更多
Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional stud...Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional studies remain challenging.To promote functional genomic studies of Chinese cabbage,we generated an ethyl methane sulfonate(EMS)mutant population of~8000 M_(2) plants using the double haploid inbred line A03 as the parent.The genome of A03 was sequenced and used as a reference for high-throughput functional characterization of gene mutations at the whole-genome level.A total of 300 M_(2) to M_(5) EMS mutants were phenotypically screened and then sequenced,revealing 750629 SNPs and 46272 InDel mutations that cover 98.27%of all predicted genes in the A03 genome.A forward-genetics approach was successfully used to identify two genes with chloroplast-related functions that are responsible for the yellow leaf mutant trait.A reverse-genetics approach was also used to identify associations between mutations in five genes of the glucosinolate biosynthetic pathway and variations in glucosinolate content of the mutant plants.In addition,we built the Chinese cabbage EMS mutation database(CCEMD,www.bioinformaticslab.cn/EMSmutation/home)to increase the usability of this mutant population resource.In summary,we performed large-scale screening of a heading Chinese cabbage EMS mutant collection at the phenotypic and genotypic levels,which will facilitate gene mining of Chinese cabbage and might also be useful for the study of other Brassica crops.展开更多
基金supported by the State Key Program of National Natural Science Foundation of China(Grant Nos.31930098,31772324)Hebei Provincial Natural Science Fund for Distinguished Young(Grant No.C2020204063)+6 种基金Natural Science Foundation and basic research project in Hebei Province(Grant No.18966925D)the Innovative Research Group Project of Hebei Natural Science Foundation(Grant No.C2020204111)the Agricultural Science and Technology Innovation Program of CAAS(Grant No.CAASXTCX2019025)the National Natural Science Foundation of China(Grant No.31672151)the Science and Technology Support Program of Hebei(Grant No.16226304D-2)Science and Technology Research Project of Universities in Hebei Province(BJ2019020)the International Science and Technology Cooperation base Special Project of Hebei(Grant No.20592901D)。
文摘Flowering time is an important agronomic trait of Chinese cabbage with late flowering being a primary breeding objective.In our previous work,we obtained Chinese cabbage-cabbage translocation lines that contained several beneficial cabbage genes.Cabbage-specific molecular markers show that these genes were coming from chromosome C01 of cabbage.In this study,we investigated the inheritance of flowering time in a couple of translocation lines and analyzed the transmission rate of molecular markers in the offspring.Consequently,we obtained the late flowering Chinese cabbage-cabbage translocation line‘AT7–4’in which the flowering time was later than that of‘85–1’by about 7 days under 4-week vernalization.Based on previous studies of the genomes of Chinese cabbage and cabbage,we located the cabbage-specific molecular markers that were closely linked at the top of the chromosome A01 in the F2mapping population generated by self-crossing F1s derived from a cross between the translocation line‘AT7–4’and Chinese cabbage‘14–36’.Five flowering-related genes in the alien fragment were found by functional annotation and their molecular markers were developed.This study lays the foundation for the future improvement of Chinese cabbage varieties using A-C translocation lines.
基金This work was supported by the Natural Science Foundation of Hebei Province(C2021204015)the 2021 Project for the Introduction of Oversea Students in Hebei Province(C20210510)+4 种基金science and technology research projects of colleges and universities in Hebei Province(ZD2022111)the Introduction of Talents Start-up fund of the State Key Laboratory of North China Crop Improvement and Regulation(NCCIR2020RC-13)the Introduction of Talents Start-up fund of Hebei Agricultural University(YJ2020067)the Hebei Fruit Vegetables Seed Industry Science and Technology Innovation Team Project(21326309D)the Vegetable Innovation Team Project of Hebei Modern Agricultural Industrial Technology System(HBCT2018030203).
文摘Fleshy fruit shape is an important external quality trait influencing the usage of fruits and consumer preference.Thus,modification of fruit shape has become one of the major objectives for crop improvement.However,the underlying mechanisms of fruit shape regulation are poorly understood.In this review we summarize recent progress in the genetic basis of fleshy fruit shape regulation using tomato,cucumber,and peach as examples.Comparative analyses suggest that the OFP-TRM(OVATE Family Protein-TONNEAU1 Recruiting Motif)and IQD(IQ67 domain)pathways are probably conserved in regulating fruit shape by primarily modulating cell division patterns across fleshy fruit species.Interestingly,cucumber homologs of FRUITFULL(FUL1),CRABS CLAW(CRC)and 1-aminocyclopropane-1-carboxylate synthase 2(ACS2)were found to regulate fruit elongation.We also outline the recent progress in fruit shape regulation mediated by OFP-TRM and IQD pathways in Arabidopsis and rice,and propose that the OFP-TRM pathway and IQD pathway coordinate regulate fruit shape through integration of phytohormones,including brassinosteroids,gibberellic acids,and auxin,and microtubule organization.In addition,functional redundancy and divergence of the members of each of the OFP,TRM,and IQD families are also shown.This review provides a general overview of current knowledge in fruit shape regulation and discusses the possible mechanisms that need to be addressed in future studies.
基金This work was supported by the National Natural Science Foundation of China(Grant Nos.31930098 and 32172560)the Hebei Provincial Natural Science Fund for Distinguished Young Scholars(Grant No.C2020204063)the Innovative Research Group Project of Hebei Natural Science Foundation(Grant No.C2020204111).
文摘The genus Brassica contains a rich diversity of species and morphological types,including leaf,root,and oil crops,all of which show substantial phenotypic variation.Both Chinese cabbage and cabbage are typical leaf-type crops with normal roots.We created translocation lines based on interspecific crosses between Chinese cabbage and cabbage and identified qdh225,which exhibited a swollen-root phenotype.The swollen root of qdh225 contained a large number of granular substances,and the formation of its irregular morphological tissue was caused by a thickening of the phloem.Transcriptomic and metabolomic data suggested that differential expression of genes encoding nine types of enzymes involved in starch and sucrose metabolism caused changes in starch synthesis and degradation in the swollen root.These genes jointly regulated sucrose and starch levels,leading to significant enrichment of starch and soluble proteins in the swollen root and a reduction in the content of soluble sugars such as d-glucose and trehalose 6-phosphate.A significant increase in auxin(IAA)and abscisic acid(ABA)contents and a decrease in gibberellin(GA)content in the swollen root likely promoted the differential expression of genes associated with hormone signal transduction,thereby regulating the development of the swollen root.Taken together,our data suggest that accumulation of IAA and ABA and reduction in GA promote swollen root formation by regulating hormone-mediated signaling,leading to a thickening of phloem,root enlargement,and substantial accumulation of starch and soluble proteins.The latter provide materials,energy,and nutrient sources for the development of swollen roots.
文摘Objective: To evaluate the diagnostic value and safety of CT and ultrasound-guided PTNB for peripheral type of occupying lung lesions by meta-analysis and clinical study. Methods: 1) The target data of randomized controlled trials of CT and ultrasound-guided PTNB for peripheral type of occupying lung lesions were extracted by computer search of foreign PubMed, Embase, the Cochrane Library, Web of Science databases, since the establishment of the database. Cochrane quality assessment criteria were used for evaluation. Statistical analysis was performed using Review Manager 5.3 software. 2) To retrospectively study the diagnosis rate and complication rate of patients, undergoing CT or ultrasound-guided PTNB in the First Affiliated Hospital of Dali University from January 2015 to December 2020. Results: Meta-analysis included 7 papers with a total of 1177 patients including 502 patients in the ultrasound group and 675 patients in the CT group. Meta-analysis results showed that there was no difference in the diagnosis rate of PTNB guided by ultrasound and CT. The incidence of postoperative complications was higher in the CT group than in the ultrasound group. The incidence of postoperative pneumothorax was higher in the CT group than in the ultrasound group, and there was no difference in the incidence of postoperative bleeding. 3) Clinical study results show that the puncture success rate was 100% in both of the ultrasound and CT groups, the pathological diagnosis rate was 85.48% in the ultrasound group and 91.67% in the CT group, and there was no difference in the overall complication rate between the two puncture groups. Conclusion: Either ultrasound or CT-guided PTNB is a safe and effective clinical diagnostic method for the diagnosis of peripheral pulmonary occupations.
基金supported in part by the National Natural Science Foundation of China under Grant Nos.61427802,31727901,61625103,61501032,61471038the Chang Jiang Scholars Program(T2012122)+1 种基金part by the 111 project of China under Grant B14010supported by the Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China
文摘Radar is an electronic device that uses radio waves to determine the range, angle, or velocity of objects. Real-time signal and information processor is an important module for real-time positioning, imaging, detection and recognition of targets. With the development of ultra-wideband technology, synthetic aperture technology, signal and information processing technology, the radar coverage, detection accuracy and resolution have been greatly improved, especially in terms of one-dimensional(1D) high-resolution radar detection, tracking, recognition, and two-dimensional(2D) synthetic aperture radar imaging technology. Meanwhile, for the application of radar detection and remote sensing with high resolution and wide swath, the amount of data has been greatly increased. Therefore, the radar is required to have low-latency and real-time processing capability under the constraints of size, weight and power consumption. This paper systematically introduces the new technology of high resolution radar and real-time signal and information processing. The key problems and solutions are discussed, including the detection and tracking of 1D high-resolution radar, the accurate signal modeling and wide-swath imaging for geosynchronous orbit synthetic aperture radar, and real-time signal and information processing architecture and efficient algorithms. Finally, the latest research progress and representative results are presented, and the development trends are prospected.
基金supported by the National Key R&D of China[Grant No.2017YFD0101802,2016YFD0100204-17]the National Natural Science Foundation of China[Grant No.31672151 and 31772324]+6 种基金the Hundred-Talents Program of Hebei Universities[Grant No.SLRC2017040]the Basic Research Program of Hebei[Grant No.18966925D]the Natural Science Foundation of Hebei[C2016204170]the Science and Technology Support Program of Hebei[16226304D-2]the Science and Technology Research Project of Hebei Colleges and Universities[Grant No.QN2017084 and ZD2017236]the International Cooperation Project in the Science and Technology Support Program of Hebei[Grant No.17396315D]the Graduate Student Innovation Fund Project in Hebei Province[CXZZBS2017067].
文摘Pectobacterium carotovorum ssp.carotovorum(Pcc)is a necrotrophic bacterial species that causes soft rot disease in Chinese cabbage.In this study,plants harboring the resistant mutant sr gene,which confers resistance against Pcc,were screened from an 800 M2 population mutated by ethyl methane sulfonate(EMS)and scored in vitro and in vivo for lesion size.The transcript profiles showed~512 differentially expressed genes(DEGs)between sr and WT plants occurring between 6 and 12 h postinoculation(hpi),which corresponded to the important defense regulation period(resistance)to Pcc in Chinese cabbage.The downstream defense genes(CPK,CML,RBOH MPK3,and MPK4)of pathogen pattern-triggered immunity(PTI)were strongly activated during infection at 12 hpi in resistant mutant sr;PTI appears to be central to plant defense against Pcc via recognition by three putative pattern recognition receptors(PRRs;BrLYM1-BrCERK1,BrBKK1/SERK4-PEPR1,BrWAKs).Pcc triggered the upregulation of the jasmonic acid(JA)and ethylene(ET)biosynthesis genes in mutant sr,but auxins and other hormones may have affected some negative signals.Endogenous hormones(auxins,JAs,and SA),as well as exogenous auxins(MEJA and BTH),were also verified as functioning in the immune system.Concurrently,the expression of glucosinolate and lignin biosynthesis genes was increased at 12 hpi in resistant mutant sr,and the accumulation of glucosinolate and lignin also indicated that these genes have a functional defensive role against Pcc.Our study provides valuable information and elucidates the resistance mechanism of Chinese cabbage against Pcc infection.
基金the National Natural Science Foundation of China(Grant Nos.32002054,31930098,and 31801857)he National Key R&D of China(Grant No.2016YFD0100204-17)+2 种基金the Science and Technology Support Program of Hebei(Grant No.360108)the Natural Science Foundation of Hebei(Grant No.C2020204122)the International Cooperation Project in the Science and Technology Support Program of Hebei(Grant Nos.2019YX023A and 17396315D)。
文摘The agricultural and consumer quality of Chinese cabbage is determined by its shape.The shape is defined by the folding of the heading leaves,which defines the head top shape(HTS).The overlapping HTS,in which the heading leaves curve inward and overlap at the top,is the shape preferred by consumers.To understand the genetic regulation of HTS,we generated a large segregating F 2 population from a cross between pak choi and Chinese cabbage,with phenotypes ranging from nonheading to heading with either outward curving or inward curving overlapping heading leaves.HTS was correlated with plant height,outer/rosette leaf length,and petiole length.A high-density genetic map was constructed.Quantitative trait locus(QTL)analysis resulted in the identification of 22 QTLs for leafy head-related traits,which included five HTS QTLs.Bulked segregant analysis(BSA)was used to confirm HTS QTLs and identify candidate genes based on informative single-nucleotide polymorphisms.Interestingly,the HTS QTLs colocalized with QTLs for plant height,outer/rosette leaf,and petiole length,consistent with the observed phenotypic correlations.Combined QTL analysis and BSA laid a foundation for molecular marker-assisted breeding of Chinese cabbage HTS and directions for further research on the genetic regulation of this trait.
基金Supported by the National Nature Science Foundation of China(Grant No.10901098 and No.11271239)
文摘The antipode of a Yetter-Drinfeld Hopf algebra is an anti-algebra and anti-coalgebra map is proved. It is also proved that the tensor algebra of Yetter-Drinfeld Hopf module is a Yetter-Drinfeld Hopf algebra.
文摘In this paper, the fundamental theorem of Yetter-Drinfeld Hopf module is proved. As applications, the freedom of tensor and twisted tensor of two Yetter-Drinfeld Hopf algebras is given. Let A be a Yetter-Drinfeld Hopf algebra. It is proved that the category of A-bimodule is equivalent to the category of ? -twisted module.
基金supported by the fundings from the National Natural Science Foundation of China (31930098,32172594)Hebei Provincial Department of Human Resources and Social Security (C20210363)+1 种基金Hebei Provincial Department of Science and Technology (216Z2904G)the Natural Science Foundation of Heibei (C2020204111).
文摘With the complementation of whole-genome sequencing of Chinese cabbage,it is necessary to develop genome-wide Simple Sequence Repeat(SSR)markers and analyse their characteristics,which will bring a revolution in the molecular marker-assisted breeding of Chinese cabbage.In this study distribution and characteristics of SSR loci in the genome of Chinese cabbage'Chiifu'was analyzed.Finally,a total of 173,892 SSR markers that occurred in 10 chromosomes were identified,and Perfect Repeat Motifs(P type)which is a main form of SSR loci with a mean distance of 1.89 kb/SSR was identified.Among them,mono-,di-,and tri-nucleotide repeat motifs were equally distributed across chromosomes compared with tetra-,penta-,and hexa-nucleotide repeat motifs.The largest proportion of SSR loci consisted of mono-and dinucleotide repeat motifs,which accounted for 93.04%of the total SSR loci.A/T and AT/TA were the most abundant motifs,accounting for 97.61%and 64.05%of the mono-and dinucleotide repeats,respectively.Furthermore,200 SSR markers were used for the amplification of 12 Chinese cabbage germplasm,in which 30 were highly polymorphic.These SSR markers were further used for the association analyses of flowering time of 190 Chinese cabbage accessions.The results revealed that 9 and 10 alleles were associated with bolting and flowering time,respectivley.The results show that development of SSR markers is feasible and useful in marker assisted selection of Chinese cabbage.
基金Project supported by the National Key R&D Program of China(2019YFC1904100,2019YFC1904102,2019YFC1903900,2019YFC1903902)the National Natural Science Foundation of China(U20A20132,52106180,22076136)+1 种基金the Hebei Province Major Scientific and Technological Achievement Transformation Fund Support Project(2021004012 A)the Central Government Guides the Local Science and Technology Development Fund Project(206Z3702G).
文摘Due to strong synergistic effect of the elements,a series of XEuMnO_(x) ternary oxides(X=Ce,Ni,Co,Sb,Sn,Mo) were synthesized by one-pot co-precipitation method,and composite components were identified and optimized to maintain high activity and superior SO_(2)and H_(2)O endurance in selective catalytic reduction of NO_(x)with NH_(3)(NH_(3)-SCR).NO_(x)conversion of CeEuMnO_(x) ternary oxide catalysts attains more than 90% at 100-250℃,and finally achieves 74%under existence of 50×10^(-6)SO_(2) and 10 vol% H_(2)O at 230℃.The facile electron transfer through redox cycle of Mn^(3+)+Ce^(4+)■Mn4++Ce^(3+) and enhanced oxygen mobility can promote formation of more Mn species in high oxidation state and chemisorbed oxygen,accelerating oxidation of NO and the adsorbed NO_(2) formed can facilitate"fast SCR"reaction to improve low-temperature activity.In situ diffuse reflectance infrared Fourier transform spectroscopy(DRIFTS)study reveals that addition of Ce to EuMnO_(x)catalyst boosts adsorption of NH_(3)and NO_(x)species.NH_(3)species are activated as crucial intermediate(NH_(2))to promote NH_(3)-SCR reaction.This research provides a novel material for practical deNO_(x)application of stationary source combustion flue gas in the future.
基金supported by the NSF of China(grants No.11971289,No.11771176)Wang Yanhua was supported by the NSF of China(grant No.11971289)+1 种基金Zhao Zhibing was supported by the NSF of China(grant No.11571329)the Project of University Natural Science Research of Anhui Province(grant No.KJ2019A0007).
文摘In this paper,we describe multiplicative derivations on the set of all rank-s matrices of Mn(K)over a field K with a relatively small integer s.Concretely,for fixed integers n,s satisfying 1≤s≤n/2 and n≥2,we prove that if a map d:Mn(K)→Mn(K)satisfiesδ(xy)=δ(x)y+xδ(y)for any two rank-s matrices,y∈Mn(K),then there exists a derivation D of Mn(K)such thatδ(x)=D(x)for each rank-k matrix a E Mn(K)with 0≤k≤s.As an application,we prove that a multiplicative derivation on a special subset of Mn(K)must be a derivation.
基金Supported by the National Natural Science Foundation of China (No. 81173514,No.81001673)the Xijing Research Boosting Program (No. XJZT10D02)the Excellent Civil Service Training Fund of Fourth Military Medical University(No. 4138C4IDK6)
文摘OBJECTIVE: To determine the cardioprotective effect of magnesium lithospermate B (MLB) on myocardial ischemia/reperfusion (MI/R) injury and to investigate the antioxidant potential in vivo and in vitro. METHODS: MI/R injury was induced by the occlusion of left anterior descending coronary artery for 30 min followed by reperfusion for 3 h in rats. After reperfusion, hearts were harvested to assess infarct size, histopathological damages, the levels of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) and malondialdehyde (MDA). Blood samples were col- lected to determine serum levels of creatine kinase-MB (CK-MB), cardiac troponin (cTnI) and lactate dehydrogenase (LDH). Furthermore, simulatedischemia/reperfusion (SI/R) injury in vitro was established by oxygen and glucose deprivation (OGD) for 2 h followed by 24-hour recovery period in cardiomyocytes. The activity of LDH in the cultured supernatant and the levels of intracellular reactive oxygen species (ROS), SOD and MDA in cardiomyocytes were also measured. Finally, cardiomyocytes apoptosis was determined with flow cytometry. RESULTS: MLB significantly limited infarct size, ameliorated histopathological damages and prevented leakage of CK-MB, cTnI and LDH. Additionally, SOD, CAT, GPx and GSH activities were notably increased by MLB, along with the MDA content decreased as compared with the model group in rats. In vitro study, MLB also decreased LDH activity in the cultured supernatant, increased SOD activity in cardiomyocytes, reduced intracellular ROS and MDA levels, and significantly suppressed cardiomyocytes apoptosis. CONCLUSION: MLB possessed remarkably cardioprotective effects on MI/R injury in vivo and in vitro. The protection of MLB may contribute to its antioxidant properties.
基金Project supported by AsiaLink Project "Algebras and Representations in China and Europe" ASI/B7-301/98/679-11 and the National Natural Science Foundation of China (No.10271113).
基金supported by the Innovative Research Group Project of Hebei Natural Science Foundation(grant no.C2020204111)the National Natural Science Foundation of China(grant nos.32002054,31902005,31930098,31972411,31722048,31872947)+7 种基金the International Science and Technology Cooperation base Special Project of Hebei(grant no.20592901D)Key R&D Project of Hebei(grant no.21326344D)the Natural Science Foundation of Hebei(grant nos.C2020204122,C2021204163)the Introducing Foreign Intellectual Project of Hebei(grant no.2019YX023A)the Post doctoral Science Foundation of China(grant no.2020M680901)the Science and Technology Research Project of University in Hebei(grant no.BJ2019020)the Post-graduate's Innovation Fund Project of Hebei(grant no.CXZZBS2017069)the State Key Laboratory of North China Crop Improvement and Regulation(NCCIR20202KF-2).
文摘Chinese cabbage(Brassica rapa ssp.pekinensis)is an economically important vegetable crop throughout the world,especially in Asia.High-quality genome sequences are available for Chinese cabbage,but gene functional studies remain challenging.To promote functional genomic studies of Chinese cabbage,we generated an ethyl methane sulfonate(EMS)mutant population of~8000 M_(2) plants using the double haploid inbred line A03 as the parent.The genome of A03 was sequenced and used as a reference for high-throughput functional characterization of gene mutations at the whole-genome level.A total of 300 M_(2) to M_(5) EMS mutants were phenotypically screened and then sequenced,revealing 750629 SNPs and 46272 InDel mutations that cover 98.27%of all predicted genes in the A03 genome.A forward-genetics approach was successfully used to identify two genes with chloroplast-related functions that are responsible for the yellow leaf mutant trait.A reverse-genetics approach was also used to identify associations between mutations in five genes of the glucosinolate biosynthetic pathway and variations in glucosinolate content of the mutant plants.In addition,we built the Chinese cabbage EMS mutation database(CCEMD,www.bioinformaticslab.cn/EMSmutation/home)to increase the usability of this mutant population resource.In summary,we performed large-scale screening of a heading Chinese cabbage EMS mutant collection at the phenotypic and genotypic levels,which will facilitate gene mining of Chinese cabbage and might also be useful for the study of other Brassica crops.