In this work, we report the synthesis of holmium（III）-doped carbon nanodots（Ho BCDs） as fluorescence/magnetic resonance（FL/MR） dual-modal imaging probes via a facile hydrothermal process using citrate acid（CA）...In this work, we report the synthesis of holmium（III）-doped carbon nanodots（Ho BCDs） as fluorescence/magnetic resonance（FL/MR） dual-modal imaging probes via a facile hydrothermal process using citrate acid（CA）, branched-polyethylenimine（BPEI） and diethylenetriamine pentaacetic acid hydrate holmium（III） dihydrogen salt（Ho-DTPA） as carbon source, passivating reagent and holmium source, respectively.The thus prepared Ho BCDs exhibited ultra-small particle size（~4 nm）, high water solubility and bright fluorescence with an absolute quantum yield of 8%. Additionally, grey-scaled T_1-weighted images of Ho BCDs solution appeared to be apparently brighter than that of deionized water and un-doped blue carbon nanodots（BCDs） solution. In addition, in vitro toxicity assay validated superior biocompatibility of Ho BCDs. Using He La cells as models, Ho BCDs-treated cells were observed to emit blue fluorescence located both in plasma and nucleus, and presented positive contrast enhancement in T_1-weighted images, suggesting their potentials for practical biomedical applications.展开更多
基金supported by grants from Tip-top Scientific and Technical Innovative Youth Talents of Guangdong special support program (No. 2014TQO1R417)the Fundamental Research Funds for the Central Universities (No. 171gjc09)Shenzhen Basic Research Program(No.JCYJ20170307140752183)
文摘In this work, we report the synthesis of holmium（III）-doped carbon nanodots（Ho BCDs） as fluorescence/magnetic resonance（FL/MR） dual-modal imaging probes via a facile hydrothermal process using citrate acid（CA）, branched-polyethylenimine（BPEI） and diethylenetriamine pentaacetic acid hydrate holmium（III） dihydrogen salt（Ho-DTPA） as carbon source, passivating reagent and holmium source, respectively.The thus prepared Ho BCDs exhibited ultra-small particle size（~4 nm）, high water solubility and bright fluorescence with an absolute quantum yield of 8%. Additionally, grey-scaled T_1-weighted images of Ho BCDs solution appeared to be apparently brighter than that of deionized water and un-doped blue carbon nanodots（BCDs） solution. In addition, in vitro toxicity assay validated superior biocompatibility of Ho BCDs. Using He La cells as models, Ho BCDs-treated cells were observed to emit blue fluorescence located both in plasma and nucleus, and presented positive contrast enhancement in T_1-weighted images, suggesting their potentials for practical biomedical applications.
