Bone marrow mesenchymal stem cell transplantation for treatment of spinal cord injury An in vivo magnetic resonance imaging tracking study

Non-invasive tracing in vivo can be used to observe the migration and distnbution of grafted stem cells, and can provide experimental evidence for treatment. This study utilized adenovirus-carrying enhanced green fluorescent protein （AD5/F35-eGFP） and superparamagnetic iron oxide （SPIO）-Iabeled bone marrow mesenchymal stem cells （BMSCs）. BMSCs, double-labeled by AD5/F35-eGFP and SPIO, were transplanted into rats with spinal cord injury via the subarachnoid space. MRI tracing results demonstrated that BMSCs migrated to the injured spinal cord over time （T2 hypointensity signals）. This result was verified by immunofluorescence. These results indicate that MRI can be utilized to trace in vivo the SPIO-labeled BMSCs after grafting.

Resveratrol improves cognition and reduces oxidative stress in rats with vascular dementia

Resveratrol possesses beneficial biological effects, which include anti-oxidant, anti-inflammatory and anti-carcinogenic properties. Recently, resveratrol has been shown to exhibit neuroprotective effects in models of Parkinson＇s disease, cerebral ischemia and Alzheimer＇s disease. However, its effects on vascular dementia remain unclear. The present study established a rat model of vascular dementia using permanent bilateral common carotid artery occlusion. At 8-12 weeks after model induction, rats were intragastrically administered 25 mg/kg resveratrol daily. Our results found that resveratrol shortened the escape latency and escape distances in the Morris water maze, and pro- longed the time spent percentage and swimming distance percentage in the target quadrant during the probe test, indicating that resveratrol improved learning and memory ability in vascular dementia rats. Further experiments found that resveratrol decreased malonyldialdehyde levels, and increased superoxide dismutase activity and glutathione levels in the hippocampus and cerebral cortex of vascular dementia rats. These results confirmed that the neuroprotective effects of resveratrol on vascular dementia were associated with its anti-oxidant properties.

Gastrodin blocks neural stem cell differentiation into glial cells mediated by kainic acid

Kainic acid can simulate excitatory amino acids in vitro. Neural stem cells, isolated from newborn Wistar rats, were cultured in vitro and exposed to 100 4 000 #M kainic acid for 7 days to induce neuronal cell differentiation, causing the number of astrocytes to be significantly increased. Treatment with a combination of 0.5 mg/L gastrodin and kainic acid also caused the number of differentiated neurons to be significantly increased compared with treatment with kainic acid alone Experimental findings suggest that gastrodin reduces the excitability of kainic acid and induces neural stem cell differentiation into neurons.

Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cell transplantation improves hypoxic-ischemic brain injury

Human insulin-like growth factor 1-transfected umbilical cord blood neural stem cells were transplanted into a hypoxic-ischemic neonatal rat model via the tail vein. BrdU-positive cells at day 7 post-transplantation, as well as nestin- and neuron specific enolase-positive cells at day 14 were increased compared with those of the single neural stem cell transplantation group. In addition, the proportion of neuronal differentiation was enhanced. The genetically modified cell-transplanted rats exhibited enhanced performance in correctly crossing a Y-maze and climbing an angled slope compared with those of the single neural stem cell transplantation group. These results showed that human insulin-like growth factor 1-transfected neural stem cell transplantation promotes the recovery of the leaming, memory and motor functions in hypoxic-ischemic rats.

Review of research on intelligent diagnosis of oil transfer pump malfunction

Oil transfer pump is the key dynamic equipment in the process of oil and gas gathering and transportation,and its working reliability directly affects the safety of oil and gas storage and transportation.Intelligent diagnosis is a key technical method to reduce failure rate of oil transfer pump,ensure the safety of gathering and transportation process,and avoid major safety accidents caused by oil transfer pump failure.Various oil transfer pumps have been emerged in recent decades,and the common fault types and characteristics of oil transfer pump have been brought out in the review.This article highlights on the research of the fault signal and processing methods of oil transfer pump.Firstly,the fault signal of the oil transfer pump is discussed and the advantages and disadvantages of different signal extraction are analyzed.Secondly,the intelligent diagnosis method of oil transfer pump and the shortcomings of the existing methods are pointed out.Finally,the conclusions are given and the future development perspectives of oil transfer pumps are suggested.The main contribution of this review is to give a syn-thetic understanding on oil transfer pumps.