A compact volume holographic imaging(VHI)method that can detect fluorescence objects located in diffusive medium in spectral selective imaging manner is presented.The enlargement of lateralfield of view of the VHI sys...A compact volume holographic imaging(VHI)method that can detect fluorescence objects located in diffusive medium in spectral selective imaging manner is presented.The enlargement of lateralfield of view of the VHI system is realized by using broadband illumination and demagnification optics.Each target spectrum of°uorescence emitting from a di®usive medium is probed by tuning the inclination angle of the transmission volume holographic grating(VHG).With the use of the single transmission VHG,fluorescence images with different spectrum are obtained sequentially and precise three-dimensional(3D)information of deep fluorescent objects located in a diffusive medium can be reconstructed from these images.The results of phantom experiments demonstrate that two fluorescent objects with a sub-millimeter distance can be resolved by spectral selective imaging.展开更多
基金This work is supported by the National Basic Research Program of China(973)under Grant No.2011CB707701the National Natural Science Foundation of China under Grant Nos.61361160418,61322101,81227901,81271617,and 61401246the National Major Scientific Instrument and Equipment Development Project under Grant No.2011YQ030114.
文摘A compact volume holographic imaging(VHI)method that can detect fluorescence objects located in diffusive medium in spectral selective imaging manner is presented.The enlargement of lateralfield of view of the VHI system is realized by using broadband illumination and demagnification optics.Each target spectrum of°uorescence emitting from a di®usive medium is probed by tuning the inclination angle of the transmission volume holographic grating(VHG).With the use of the single transmission VHG,fluorescence images with different spectrum are obtained sequentially and precise three-dimensional(3D)information of deep fluorescent objects located in a diffusive medium can be reconstructed from these images.The results of phantom experiments demonstrate that two fluorescent objects with a sub-millimeter distance can be resolved by spectral selective imaging.
