[ Objective] This study aimed to provide theoretical reference for the extraction of theabrownine from Liubao tea, determination of the biological activity, and development and utilization of theabrownine. [Method] Or...[ Objective] This study aimed to provide theoretical reference for the extraction of theabrownine from Liubao tea, determination of the biological activity, and development and utilization of theabrownine. [Method] Orthogonal experimental design was adopted to investigate the effects of solid-liquid ratio, extraction temperature, extraction duration, and extraction frequency on the yield of theabrownine extracted from Liubao tea. The extraction technology was optimized, and the antioxidant activity of theabrownine was analyzed. [ Result] The results showed that extraction frequency and extraction temperature had significant effects on the yield of theabrownine extracted from Liubao tea, while solid-liquid ratio and extraction duration had no significant effect. The optimal conditions for extracting theabrownine from Liubao tea were determined as follows : solid-liquid ratio was 1 : 30, extraction temperature was 100℃, extraction duration was 80 min, and extraction frequency was 4 times. Under these conditions, the yield of theabrownine reached 22.92%. Results of antioxidant activity determination showed that hydroxyl radical-scavenging activity of theabrownine was as high as 74%, and the scavenging effects was significantly higher than that of Vitamin C under low concentrations; nitroso-scavenging activity of theabrownine reached 73%, with no significant difference from that of Vitamin C; superoxide anion free radical-scavenging activity of theabrownine could be above 60%, which was lower than that of Vitamin C. [Condusion] Liubao tea contains abundant theabrownine that has relatively high antioxidant activity with good prospects in antioxidant application, which lays solid foundation for in-depth development of Liubao tea.展开更多
This study introduced a two-stage cultivation method for sweet pepper seedlings, integrating the strengths of a closed plant factory and solar greenhouse, to mitigate the environmental constraints in Northeast China d...This study introduced a two-stage cultivation method for sweet pepper seedlings, integrating the strengths of a closed plant factory and solar greenhouse, to mitigate the environmental constraints in Northeast China during the early spring season. In the first stage, seedlings were cultivated in a closed plant factory, followed by a second stage in a solar greenhouse. Four treatments- T1 (9 and 36 d), T2 (12 and 33 d), T3 (15 and 30 d), and T4 (18 and 27 d) - were designed for the first and second stages, respectively, with solar greenhouse-only approach serving as the control (CK). The findings reveal that the two-stage methodology significantly outperformed the control across multiple metrics, including seedling health index, chlorophyll content, photosynthetic capacity, yield, etc. Specifically, T3 emerged as optimal, boosting the health index by 38.59%, elevating chlorophyll content by 39.61%, increasing net photosynthesis by 34.61%, and augmenting yield per plant by 40.67%. Additionally, T3 expedited the time to harvest by 25 d compared to the control. Although the seedling cost for T3 was 0.12 RMB yuan higher, the benefits offset the additional investment. In conclusion, the two-stage cultivation method effectively leverages the advantages of both closed-plant factories and solar greenhouses, resulting in superior seedling quality compared to using only solar greenhouses. It offers a practical and economically viable solution for enhancing the quality and yield of sweet pepper seedlings, thus contributing to the progress in the field of facility seedling cultivation research.展开更多
基金Supported by Scientific and Technological Development Project of Guangxi Zhuang Autonomous Region(GKN1348022-4)General Project of Scientific Research Fund of the Education Department of Guangxi Zhuang Autonomous Region(201012MS138)
文摘[ Objective] This study aimed to provide theoretical reference for the extraction of theabrownine from Liubao tea, determination of the biological activity, and development and utilization of theabrownine. [Method] Orthogonal experimental design was adopted to investigate the effects of solid-liquid ratio, extraction temperature, extraction duration, and extraction frequency on the yield of theabrownine extracted from Liubao tea. The extraction technology was optimized, and the antioxidant activity of theabrownine was analyzed. [ Result] The results showed that extraction frequency and extraction temperature had significant effects on the yield of theabrownine extracted from Liubao tea, while solid-liquid ratio and extraction duration had no significant effect. The optimal conditions for extracting theabrownine from Liubao tea were determined as follows : solid-liquid ratio was 1 : 30, extraction temperature was 100℃, extraction duration was 80 min, and extraction frequency was 4 times. Under these conditions, the yield of theabrownine reached 22.92%. Results of antioxidant activity determination showed that hydroxyl radical-scavenging activity of theabrownine was as high as 74%, and the scavenging effects was significantly higher than that of Vitamin C under low concentrations; nitroso-scavenging activity of theabrownine reached 73%, with no significant difference from that of Vitamin C; superoxide anion free radical-scavenging activity of theabrownine could be above 60%, which was lower than that of Vitamin C. [Condusion] Liubao tea contains abundant theabrownine that has relatively high antioxidant activity with good prospects in antioxidant application, which lays solid foundation for in-depth development of Liubao tea.
基金supported by the China Agricultural Research System of MOF and MARA (Grant No.CARS-24-G-05)Jilin Province Science and Technology Development Plan Talent Special Project (Grant No.232695HJ0101110676).
文摘This study introduced a two-stage cultivation method for sweet pepper seedlings, integrating the strengths of a closed plant factory and solar greenhouse, to mitigate the environmental constraints in Northeast China during the early spring season. In the first stage, seedlings were cultivated in a closed plant factory, followed by a second stage in a solar greenhouse. Four treatments- T1 (9 and 36 d), T2 (12 and 33 d), T3 (15 and 30 d), and T4 (18 and 27 d) - were designed for the first and second stages, respectively, with solar greenhouse-only approach serving as the control (CK). The findings reveal that the two-stage methodology significantly outperformed the control across multiple metrics, including seedling health index, chlorophyll content, photosynthetic capacity, yield, etc. Specifically, T3 emerged as optimal, boosting the health index by 38.59%, elevating chlorophyll content by 39.61%, increasing net photosynthesis by 34.61%, and augmenting yield per plant by 40.67%. Additionally, T3 expedited the time to harvest by 25 d compared to the control. Although the seedling cost for T3 was 0.12 RMB yuan higher, the benefits offset the additional investment. In conclusion, the two-stage cultivation method effectively leverages the advantages of both closed-plant factories and solar greenhouses, resulting in superior seedling quality compared to using only solar greenhouses. It offers a practical and economically viable solution for enhancing the quality and yield of sweet pepper seedlings, thus contributing to the progress in the field of facility seedling cultivation research.