Interferon-γis a kind of protein with a wide range of biological activities,which can regulate the immune function of the body,and can be used as an important marker to detect and treat bovine tuberculosis diseases.H...Interferon-γis a kind of protein with a wide range of biological activities,which can regulate the immune function of the body,and can be used as an important marker to detect and treat bovine tuberculosis diseases.Here,a picogram-level bovine interferon-γ(BoIFN-γ)electrochemical impedance immunosensor was constructed for the first time using mesoporous silica nanospheres(MS Ns)to immobilize specific monoclonal BoIFN-y antibodies.The MS Ns and BoIFN-γimmuno sensors were characterized using scanning electron microscopy,transmission electron microscope,nitrogen adsorption experiment,X-ray photoelectron spectra,and contact angle measurements.MSNs possess a substantial specific surface area and significant hydrophilicity,and can immobilize many antibody molecules,thereby improving detection sensitivity.The immunosensor has a linear detection range from 0.001 to 10.0 ng/mL with an exceptionally low detection limit of 0.62 pg/mL.Compared to the traditional BoIFN-γanalysis method,BoIFN-γimmunosensor presents superiorities in sensitivity,wide linear range as well as short processing time.More importantly,the BoIFN-γsensor exhibits high selectivity,reliable repeatability as well as stability,providing a promising application prospect for the early diagnosis of Mycobacterium bovis infection.展开更多
基金funded by by National Key Research and Development Program of China(2021YFD1800403)National Natural Science Foundation of China(21475116,21575125 and 81302016)+4 种基金Natural Science Foundation of Jiangsu Province(BK20221370,BK20221281)Key University Natural Science Foundation of Jiangsu Province(20KJA150004)the Project for Science and Technology of Yangzhou(YZ2022074,YZ2020076)Crosscooperation project of Subei Peoples’Hospital of Jiangsu Province(SBJC220009)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_3203)
文摘Interferon-γis a kind of protein with a wide range of biological activities,which can regulate the immune function of the body,and can be used as an important marker to detect and treat bovine tuberculosis diseases.Here,a picogram-level bovine interferon-γ(BoIFN-γ)electrochemical impedance immunosensor was constructed for the first time using mesoporous silica nanospheres(MS Ns)to immobilize specific monoclonal BoIFN-y antibodies.The MS Ns and BoIFN-γimmuno sensors were characterized using scanning electron microscopy,transmission electron microscope,nitrogen adsorption experiment,X-ray photoelectron spectra,and contact angle measurements.MSNs possess a substantial specific surface area and significant hydrophilicity,and can immobilize many antibody molecules,thereby improving detection sensitivity.The immunosensor has a linear detection range from 0.001 to 10.0 ng/mL with an exceptionally low detection limit of 0.62 pg/mL.Compared to the traditional BoIFN-γanalysis method,BoIFN-γimmunosensor presents superiorities in sensitivity,wide linear range as well as short processing time.More importantly,the BoIFN-γsensor exhibits high selectivity,reliable repeatability as well as stability,providing a promising application prospect for the early diagnosis of Mycobacterium bovis infection.
