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How mobile messaging apps users share information on WeChat: The role of demographic variables 被引量:1
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作者 Shengli DENG yanqing lin 《Chinese Journal of Library and Information Science》 2015年第4期32-49,共18页
Purpose: We conducted an empirical study to find out the role of demographic variables in affecting information sharing behaviors of college student users of WeChat.Design/methodology/approach: A questionnaire surve... Purpose: We conducted an empirical study to find out the role of demographic variables in affecting information sharing behaviors of college student users of WeChat.Design/methodology/approach: A questionnaire survey was carried out to investigate the relationship between demographic variables (gender, grade level, dating status, and singleparent family background) and information sharing behaviors of WeChat users. The participants were college students and a total of 255 valid questionnaires were collected. Data was analyzed using descriptive statistics and multiple regression analysis.Findings: Grade level and single-parent family background were found to be significantly correlated with information sharing behaviors whereas no effect of gender was found on information sharing behaviors. Dating status had no significant impact on user browsing behavior, but was related to users' publishing posts and posting replies.Implications: The study will help understand individual differences in information sharing among WeChat users.Limitations: First, the relatively small sample size is a limitation in exploring the effects of demographic variables on user information sharing behaviors. Second, this study only used questionnaire surveys to collect data and more research methods such as interviews should be adopted to improve the accuracy of the study results.Originality/value: This paper is one of the first studies to explore the relationship between demographic variables and user information sharing behaviors on WeChat. 展开更多
关键词 Demographic variable Information sharing WeChat Mobile messaging apps College student
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Selective Ensemble Learning Method for Belief-Rule-Base Classification System Based on PAES 被引量:1
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作者 Wanling Liu Weikun Wu +2 位作者 Yingming Wang Yanggeng Fu yanqing lin 《Big Data Mining and Analytics》 2019年第4期306-318,共13页
Traditional Belief-Rule-Based(BRB) ensemble learning methods integrate all of the trained sub-BRB systems to obtain better results than a single belief-rule-based system. However, as the number of BRB systems particip... Traditional Belief-Rule-Based(BRB) ensemble learning methods integrate all of the trained sub-BRB systems to obtain better results than a single belief-rule-based system. However, as the number of BRB systems participating in ensemble learning increases, a large amount of redundant sub-BRB systems are generated because of the diminishing difference between subsystems. This drastically decreases the prediction speed and increases the storage requirements for BRB systems. In order to solve these problems, this paper proposes BRBCS-PAES: a selective ensemble learning approach for BRB Classification Systems(BRBCS) based on ParetoArchived Evolutionary Strategy(PAES) multi-objective optimization. This system employs the improved Bagging algorithm to train the base classifier. For the purpose of increasing the degree of difference in the integration of the base classifier, the training set is constructed by the repeated sampling of data. In the base classifier selection stage, the trained base classifier is binary coded, and the number of base classifiers participating in integration and generalization error of the base classifier is used as the objective function for multi-objective optimization. Finally,the elite retention strategy and the adaptive mesh algorithm are adopted to produce the PAES optimal solution set.Three experimental studies on classification problems are performed to verify the effectiveness of the proposed method. The comparison results demonstrate that the proposed method can effectively reduce the number of base classifiers participating in the integration and improve the accuracy of BRBCS. 展开更多
关键词 belief-rule-base pareto-archived evolutionary strategy selective ensemble classification
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慢病毒介导N2ICD过表达的人胃癌MKN45细胞稳定株的构建及鉴定
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作者 吴丹琳 温俊杰 +9 位作者 叶健斌 薛杨 林彦青 胡冰心 罗美群 陈中标 谢金玲 宁立军 宁云山 李妍 《生物技术》 CAS 2018年第4期372-376,共5页
[目的]构建慢病毒介导Notch2受体胞内段((Notch2 intracellular domain,N2ICD)过表达的人胃癌MKN45细胞稳定株并进行鉴定。[方法]采用PCR法扩增N2ICD基因并克隆至慢病毒载体PTYE-EF1α-IRES-EGFP(简写为pLV)中,通过酶切及测序鉴定后,三... [目的]构建慢病毒介导Notch2受体胞内段((Notch2 intracellular domain,N2ICD)过表达的人胃癌MKN45细胞稳定株并进行鉴定。[方法]采用PCR法扩增N2ICD基因并克隆至慢病毒载体PTYE-EF1α-IRES-EGFP(简写为pLV)中,通过酶切及测序鉴定后,三质粒共转染HEK293T细胞包装病毒,收集且浓缩病毒毒液用于感染人胃癌MKN45细胞并筛选稳定细胞株,用Western Blot验证。[结果]成功构建慢病毒重组质粒N2ICD/pLV;包装病毒后,荧光显微镜下观察到大部分HEK293T细胞发出绿色荧光;收集病毒毒液感染MKN45细胞,显微镜下观察有部分细胞表达绿色荧光蛋白;流式分选技术筛选得到稳定细胞株,Western Blot鉴定结果显示:相比于空白对照组,稳定株的N2ICD过表达明显。[结论]成功构建慢病毒表达质粒N2ICD/pLV,并建立稳定过表达N2ICD的胃癌细胞株N2ICD/pLV-MKN45。 展开更多
关键词 NOTCH2 N2ICD 慢病毒 稳定株
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