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A review on protein based nanocarriers for polyphenols:interaction and stabilization mechanisms
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作者 Yun Xiao Talha Ahmad +4 位作者 Tarun Belwal Rana Muhammad Aadil Muhammad Siddique Limin Pang yanqun xu 《Food Innovation and Advances》 2023年第3期193-202,共10页
Protein has been used as the carrier for protecting and targeting polyphenols and increasing their shelf-life.Interactions of a protein molecule with polyphenols are important,which change functions and physiochemical... Protein has been used as the carrier for protecting and targeting polyphenols and increasing their shelf-life.Interactions of a protein molecule with polyphenols are important,which change functions and physiochemical properties of the complex and provide protection to polyphenols.Interactions between proteins and polyphenols are largely non-covalent.Factors that affect such interactions include pH,temperature,and the structure of both proteins and polyphenols.Moreover,excellent stability of polyphenols can be achieved by using nanoencapsulation techniques such as emulsion,nanohydrogel,and nanocomplex formation.The use of protein combined with other compounds such as lipids and carbohydrates was found to be the most suitable carrier for polyphenols encapsulation.This review aims to describe the interaction between proteins and polyphenols,focusing on applying nanoencapsulation for increasing stability and targeted delivery of phenolic compounds. 展开更多
关键词 INTERACTION STABILITY STABILIZATION
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Novel nanobubble technology in food science:Application and mechanism
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作者 Miral Javed Anam Matloob +3 位作者 Fatima-ezzahra Ettoumi Arooj Rehman Sheikh Ruyuan Zhang yanqun xu 《Food Innovation and Advances》 2023年第2期135-144,共10页
Nanobubble technology is one of the latest green technologies in food industry applications.Nanobubbles(NBs)are gas-filled nanoscopic bubbles with a diameter of<500 nm.The mass production of bulk nano-bubbles raise... Nanobubble technology is one of the latest green technologies in food industry applications.Nanobubbles(NBs)are gas-filled nanoscopic bubbles with a diameter of<500 nm.The mass production of bulk nano-bubbles raises growing interest due to their high stability,internal pressure,and an enormous surface-to-volume ratio.Also,they can increase surface area,alter the physicochemical characteristics of the medium,and facilitate mass transfer.Along with their size and stability,air,nitrogen,and carbon dioxide,NBs are widely recognized for their significance in food processing.The potential existence and ability of NBs to remain stable in liquids under ambient conditions have been a contentious issue for a long time due to the conventional thermodynamic theory.In this review,fundamental properties,and several generation methods of NBs have been described along with their mechanism.Moreover,NBs generation methods can produce fine and undeviating gas diffusion characteristics,which can be used to control the uniformity and textural qualities of various creamy and gel-based foods.Thus,we also described the possible applications of NBs along with their mechanism of action in extraction,freezing,foams,and film formation.The ability of NBs to impart health benefits makes them new,improved,and environmentally safe green techniques. 展开更多
关键词 MECHANISM THEORY BUBBLE
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脂多糖通过促进透明质酸受体CD44向核转移介导牙周膜细胞白细胞介素6释放
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作者 徐燕群 李平 +1 位作者 杨兴 薛慧 《中华口腔医学研究杂志(电子版)》 CAS 2023年第5期335-344,共10页
目的初步探讨炎症状态下人牙周膜细胞(hPDLC)炎症反应的分子作用机制。方法利用细胞免疫荧光法鉴定原代培养hPDLC波形丝蛋白、角蛋白,使用不同浓度(0、0.1、10和100μmol/L)的脂多糖(LPS)刺激hPDLC,流式细胞术检测细胞凋亡水平。选择不... 目的初步探讨炎症状态下人牙周膜细胞(hPDLC)炎症反应的分子作用机制。方法利用细胞免疫荧光法鉴定原代培养hPDLC波形丝蛋白、角蛋白,使用不同浓度(0、0.1、10和100μmol/L)的脂多糖(LPS)刺激hPDLC,流式细胞术检测细胞凋亡水平。选择不同的LPS刺激时间,酶联免疫检测方法(ELISA)检测细胞培养上清中透明质酸受体CD44的表达。使用ELISA、蛋白免疫印迹(Western blot)及细胞免疫荧光法等实验技术检测hPDLC在炎症环境下细胞质与细胞核中CD44、基质金属蛋白酶14(MMP-14)的表达变化,微量样本多指标流式蛋白定量技术(CBA)检测炎症因子的表达。进一步在炎症环境下加入MMP-14、Importinβ抑制剂,使用ELISA法检测细胞核内CD44的表达及定量反转录聚合酶链反应(RT-PCR)法检测细胞白细胞介素6(IL-6)mRNA表达水平。结果不同浓度(0.1、1、10、100μmol/L)LPS不会对hPDLC的凋亡产生影响。在1μmol/L LPS刺激hPDLC 4 h后,MMP-14荧光强度表达明显增强(233.75),对照组为107.53,差异有统计学意义(t=10.10,P<0.001)。CD44荧光强度为106.7,对照组为84.58,差异有统计学意义(t=3.13,P=0.02)。使用MMP-14抑制剂能够明显抑制细胞培养上清中CD44的表达,LPS+MMP-14抑制剂组较LPS组CD44表达明显下调,差异有统计学意义(t=5.03,P=0.001)。LPS刺激hPDLC会导致细胞核内CD44与Importinβ表达量增加,差异有统计学意义(P<0.001)。LPS刺激后细胞核内CD44与Importinβ也有上调表达的趋势,LPS会导致IL-6表达量增加,差异有统计学意义(P<0.001),并且LPS加入Importinβ抑制剂后细胞中IL-6 mRNA的表达量较LPS处理组明显减少,差异有统计学意义(t=16.79,P<0.001)。结论LPS能够导致hPDLC CD44、Importinβ与IL-6的上调表达,核内CD44上调表达显著,MMP-14、Importinβ抑制剂能够抑制炎症环境下CD44入核,抑制IL-6上调表达。提示在炎症状态下MMP-14与CD44参与hPDLC炎症反应,CD44能够向细胞核内转移介导IL-6的释放。 展开更多
关键词 牙周炎 人牙周膜细胞 脂多糖 透明质酸受体 CD44 白细胞介素6
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