Interference of CsGPA1,theα-submit of G protein,reduces drought tolerance in cucumber seedlings

The G proteinα-subunit,GPA1,is an integral component of several signaling pathways in plants,including response to abiotic stress.However,the molecular mechanism behind these processes remains largely unknown in the cucumber plant(Cucumis sativus L.).In order to further understand the role of CsGPA1 in cucumber under drought stress,changes in plant growth,physiological parameters,and gene expression of CsAQPs were all measured under water stress induced by polyethylene glycol(PEG)using wild type(WT)and CsGPA1-interference(RNAi)cucumber seedlings.Our results demonstrated that the RNAi plants had lower drought tolerance,displaying seriously withered leaves,lower relative growth rate,lower root-shoot ratio,and lower root activity under drought stress compared to WT plants.Physiological studies indicated that the suppression of CsGPA1 weakened drought stress tolerance due to higherwater loss rate in the leaves,higher levels of hydrogen peroxide(H2O2),increased malondialdehyde(MDA)content,lower free proline content,lower soluble sugar content,lower soluble protein content,and decreased antioxidant enzyme activities.qRT-PCR analysis demonstrated that the interference of CsGPA1 up-regulated the expression of most AQP genes(except for CsPIP2;3 in leaves)and down-regulated the expression of CsPIP1;2,CsPIP1;4,CsPIP2;1,and CsPIP2;4 in roots under drought stress when compared to WT plants.Our results demonstrated that CsGPA1 could function as a positive regulator in drought stress response by decreasing the accumulation of reactive oxygen species(ROS),improving permeable potentials,and reducing water loss in cucumber plants.

Multifaceted regulatory functions of CsBPC2 in cucumber under salt stress conditions

BASIC PENTACYSTEINE(BPC)transcription factors are essential regulators of plant growth and development.However,BPC functions and the related molecular mechanisms during cucumber(Cucumis sativus L.)responses to abiotic stresses,especially salt stress,remain unknown.We previously determined that salt stress induces CsBPC expression in cucumber.In this study,Csbpc2 transgene-free cucumber plants were created using a CRISPR/Cas9-mediated editing system to explore CsBPC functions associated with the salt stress response.The Csbpc2 mutants had a hypersensitive phenotype,with increased leaf chlorosis,decreased biomass,and increased malondialdehyde and electrolytic leakage levels under salt stress conditions.Additionally,a mutated CsBPC2 resulted in decreased proline and soluble sugar contents and antioxidant enzyme activities,which led to the accumulation of hydrogen peroxide and superoxide radicals.Furthermore,the mutation to CsBPC2 inhibited salinity-induced PM-H+-ATPase and V-H+-ATPase activities,resulting in decreased Na+efflux and increased K+efflux.These findings suggest that CsBPC2 may mediate plant salt stress resistance through its effects on osmoregulation,reactive oxygen species scavenging,and ion homeostasis-related regulatory pathways.However,CsBPC2 also affected ABA signaling.The mutation to CsBPC2 adversely affected salt-induced ABA biosynthesis and the expression of ABA signaling-related genes.Our results indicate that CsBPC2 may enhance the cucumber response to salt stress.It may also function as an important regulator of ABA biosynthesis and signal transduction.These findings will enrich our understanding of the biological functions of BPCs,especially their roles in abiotic stress responses,thereby providing the theoretical basis for improving crop salt tolerance.

Sugars promote graft union development in the heterograft of cucumber onto pumpkin

The use of heterografts is widely applied for the production of several important commercial crops,but the molecular mechanism of graft union formation remains poorly understood.Here,cucumber grafted onto pumpkin was used to study graft union development,and genome-wide tempo-spatial gene expression at the graft interface was comprehensively investigated.Histological analysis suggested that resumption of the rootstock growth occurred after both phloem and xylem reconnection,and the scion showed evident callus production compared with the rootstock 3 days after grafting.Consistently,transcriptome data revealed specific responses between the scion and rootstock in the expression of genes related to cambium development,the cell cycle,and sugar metabolism during both vascular reconnection and healing,indicating distinct mechanisms.Additionally,lower levels of sugars and significantly changed sugar enzyme activities at the graft junction were observed during vascular reconnection.Next,we found that the healing process of grafted etiolated seedlings was significantly delayed,and graft success,xylem reconnection,and the growth of grafted plants were enhanced by exogenous glucose.This demonstrates that graft union formation requires the correct sugar content.Furthermore,we also found that graft union formation was delayed with a lower energy charge by the target of rapamycin(TOR)inhibitor AZD-8055,and xylem reconnection and the growth of grafted plants were enhanced under AZD-8055 with exogenous glucose treatment.Taken together,our results reveal that sugars play a positive role in graft union formation by promoting the growth of cucumber/pumpkin and provide useful information for understanding graft union healing and the application of heterografting in the future.

南瓜高效再生体系的建立

南瓜(Cucurbitamoschata)再生率较低,为建立高效的南瓜再生体系,以南瓜子叶为外植体,进行35组不同激素浓度的不定芽诱导研究。结果表明,南瓜再生受培养基中激素浓度和配比的影响,适宜浓度6-苄氨基腺嘌呤(6-BA)能有效促进不定芽形成;单独使用脱落酸(ABA)诱导使南瓜子叶发黄,但与6-BA组合使用可显著提高外植体的再生能力,1.0mg·L–16-BA与0.5mg·L–1 ABA组合南瓜芽再生率高达90.26%。将不定芽置于MS培养基中进行生根培养,再生苗移栽易成活。从子叶接种到苗再生约需70天。