Water,minerals,nutrients,etc.,can be shared by physiological integration among inter-connected ramets of clonal plants.Nitrogen plays an important role in alleviating cadmium(Cd)stress for clonal plants.But how differ...Water,minerals,nutrients,etc.,can be shared by physiological integration among inter-connected ramets of clonal plants.Nitrogen plays an important role in alleviating cadmium(Cd)stress for clonal plants.But how different forms of nitrogen affect growth performance of clonal plants subjected to heterogeneous Cd stress still remains poorly understood.A pot experiment was conducted to investigate the differential effects of ammonium and nitrate on growth performance of Glechoma longituba under heterogeneous Cd stress.In the experiment,parent ramets of Glechoma longituba clonal fragments were respectively supplied with modified Hoagland solution containing 7.5 mM ammonium,7.5 mM nitrate or the same volume of nutrient solution without nitrogen.Cd solution with different concentrations(0,0.1 or 2.0 mM)was applied to offspring ramets of the clonal fragments.Compared with control(N-free),nitrogen addition to parent ramets,especially ammonium,significantly improved antioxidant capacity[glutathione(GSH),proline(Pro),peroxidase(POD,)superoxide dismutase(SOD)and catalase(CAT)],PSII activity[maximum quantum yield of PSII(F_(v)/F_(m))and effective quantum yield of PSII(ΦPSII)],chlorophyll content and biomass accumulation of the offspring ramets suffering from Cd stress.In addition,negative effects of nitrate on growth performance of whole clonal fragments were observed under Cd stress with high concentration(2.0 mM).Transportation or sharing of nitrogen,especially ammonium,can improve growth performance of clonal plants under heterogeneous Cd stress.The experiment provides insight into transmission mechanism of nitrogen among ramets of clonal plants suffering from heterogeneous nutrient supply.Physiological integration might be an important ecological strategy for clonal plants adapting to heterogeneous environment stress conditions.展开更多
Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-c...Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-color formation has not been reported.Thus,the pigment composition and comparative transcriptome analyses of H.discus hannai with green and gray egg color were conducted using high-performance liquid chromatography(HPLC)and RNA-Seq methods.Results show that individuals with green and gray eggs both possess the fucoxanthin.Lutein existed in gray-egged individuals,but not in green-egged individuals.In transcriptome analysis,272310 unigenes were received from 461162 transcripts with a mean length of 985 bp and N50 of 1524 bp,respectively.A total of 185 unigenes were identifi ed as diff erentially expressed genes(DEGs).The DEGs involved in“fl avin-containing compound metabolic process”,“melanosome”,“glutathione metabolism”,and“cytochrome b6f complex”were likely related to the formation of the egg color.Our results provide foundational information for the functional analysis of egg-color related genes and are benefi cial to the selective breeding of H.discus hannai.展开更多
基金This research was supported by Science and Technology Support Program of Sichuan Province(2016NYZ0021)Guizhou Environmental Protection Department(2016).
文摘Water,minerals,nutrients,etc.,can be shared by physiological integration among inter-connected ramets of clonal plants.Nitrogen plays an important role in alleviating cadmium(Cd)stress for clonal plants.But how different forms of nitrogen affect growth performance of clonal plants subjected to heterogeneous Cd stress still remains poorly understood.A pot experiment was conducted to investigate the differential effects of ammonium and nitrate on growth performance of Glechoma longituba under heterogeneous Cd stress.In the experiment,parent ramets of Glechoma longituba clonal fragments were respectively supplied with modified Hoagland solution containing 7.5 mM ammonium,7.5 mM nitrate or the same volume of nutrient solution without nitrogen.Cd solution with different concentrations(0,0.1 or 2.0 mM)was applied to offspring ramets of the clonal fragments.Compared with control(N-free),nitrogen addition to parent ramets,especially ammonium,significantly improved antioxidant capacity[glutathione(GSH),proline(Pro),peroxidase(POD,)superoxide dismutase(SOD)and catalase(CAT)],PSII activity[maximum quantum yield of PSII(F_(v)/F_(m))and effective quantum yield of PSII(ΦPSII)],chlorophyll content and biomass accumulation of the offspring ramets suffering from Cd stress.In addition,negative effects of nitrate on growth performance of whole clonal fragments were observed under Cd stress with high concentration(2.0 mM).Transportation or sharing of nitrogen,especially ammonium,can improve growth performance of clonal plants under heterogeneous Cd stress.The experiment provides insight into transmission mechanism of nitrogen among ramets of clonal plants suffering from heterogeneous nutrient supply.Physiological integration might be an important ecological strategy for clonal plants adapting to heterogeneous environment stress conditions.
基金Supported by the Seed Improvement Project of Shandong Province of China(No.2017LZGC009)the Major Scientifi c and Technological Innovation Project of Shandong Provincial Key Research and Development Program(No.2019JZZY020706)。
文摘Haliotis discus hannai is an important marine economic species in China.Its egg color was found to be associated with economic traits,which provides a new idea for breeding.However,the molecular mechanism of the egg-color formation has not been reported.Thus,the pigment composition and comparative transcriptome analyses of H.discus hannai with green and gray egg color were conducted using high-performance liquid chromatography(HPLC)and RNA-Seq methods.Results show that individuals with green and gray eggs both possess the fucoxanthin.Lutein existed in gray-egged individuals,but not in green-egged individuals.In transcriptome analysis,272310 unigenes were received from 461162 transcripts with a mean length of 985 bp and N50 of 1524 bp,respectively.A total of 185 unigenes were identifi ed as diff erentially expressed genes(DEGs).The DEGs involved in“fl avin-containing compound metabolic process”,“melanosome”,“glutathione metabolism”,and“cytochrome b6f complex”were likely related to the formation of the egg color.Our results provide foundational information for the functional analysis of egg-color related genes and are benefi cial to the selective breeding of H.discus hannai.
