Keeping steps ahead of the bacteria in the race for more efficacious antibacterial strategies is increasingly difficult with the advent of bacterial resistance genes.Herein,we engineered copper sulfide nanoclusters(Cu...Keeping steps ahead of the bacteria in the race for more efficacious antibacterial strategies is increasingly difficult with the advent of bacterial resistance genes.Herein,we engineered copper sulfide nanoclusters(CuS_(x) NCs)with variable sulfur defects for enhanced dual-treatment of bacterial infections by manipulating photothermal effects and Fenton-like activity.Next,by encasing CuS_(x) NCs with a complex mixture of amino acids and short peptides derived from Luria-Bertani bacterial culture media as a protein corona,we managed to coax E.Coli to take up these CuS_(x) NCs.As a whole,Amino-Pep-CuS_(x) NCs was perceived as a food source and actively consumed by bacteria,enhancing their effective uptake by at least 1.5-fold greater than full length BSA protein BSA-corona CuS_(x) NCs.Through strategically using defect-engineering,we successfully fine-tune photothermal effect and Fenton-like capacity of CuS_(x) NCs.Increased sulfur defects lead to reduced but sufficient heat generation under solar-light irradiation and increased production of toxic hydroxyl radicals.By fine-tuning sulfur defects during synthesis,we achieve CuS_(x) NCs with an optimal synergistic effect,significantly enhancing their bactericidal properties.These ultra-small and biodegradable CuS_(x) NCs can rapidly break down after treatment for clearance.Thus,Amino-Pep-CuS_(x) NCs demonstrate effective eradication of bacteria both in vitro and in vivo because of their relatively high uptake,optimal balanced photothermal and chemodynamic outcomes.Our study offers a straightforward and efficient method to enhance bacterial uptake of next generation of antibacterial agents.展开更多
基金the funding provided by the National University of Singapore Reimagine Grant(A-0009179-02-00,A-0009179-03-00)National Natural Science Foundation of China(82303841)+1 种基金Hunan Provincial Natural Science Foundation(2023JJ40800)the program of China Scholarships Council(No.202006090323).
文摘Keeping steps ahead of the bacteria in the race for more efficacious antibacterial strategies is increasingly difficult with the advent of bacterial resistance genes.Herein,we engineered copper sulfide nanoclusters(CuS_(x) NCs)with variable sulfur defects for enhanced dual-treatment of bacterial infections by manipulating photothermal effects and Fenton-like activity.Next,by encasing CuS_(x) NCs with a complex mixture of amino acids and short peptides derived from Luria-Bertani bacterial culture media as a protein corona,we managed to coax E.Coli to take up these CuS_(x) NCs.As a whole,Amino-Pep-CuS_(x) NCs was perceived as a food source and actively consumed by bacteria,enhancing their effective uptake by at least 1.5-fold greater than full length BSA protein BSA-corona CuS_(x) NCs.Through strategically using defect-engineering,we successfully fine-tune photothermal effect and Fenton-like capacity of CuS_(x) NCs.Increased sulfur defects lead to reduced but sufficient heat generation under solar-light irradiation and increased production of toxic hydroxyl radicals.By fine-tuning sulfur defects during synthesis,we achieve CuS_(x) NCs with an optimal synergistic effect,significantly enhancing their bactericidal properties.These ultra-small and biodegradable CuS_(x) NCs can rapidly break down after treatment for clearance.Thus,Amino-Pep-CuS_(x) NCs demonstrate effective eradication of bacteria both in vitro and in vivo because of their relatively high uptake,optimal balanced photothermal and chemodynamic outcomes.Our study offers a straightforward and efficient method to enhance bacterial uptake of next generation of antibacterial agents.
