Tree peony cultivars with yellow flowers are rare and valuable,but the molecular mechanism of pigment accumulation is not clear.In this study,the petal transcriptome of three developmental stages were sequenced to det...Tree peony cultivars with yellow flowers are rare and valuable,but the molecular mechanism of pigment accumulation is not clear.In this study,the petal transcriptome of three developmental stages were sequenced to determine the differentially expressed genes(DEGs)regulating yellow flowers color.The results showed that 10,842 and 12,022 DEGs were screened in stage 1 vs.stage 2 and in stage 2 vs.stage 3,respectively.Through analysis of flavonoid structural genes(FSGs),we found that the transcription level of DFR was very low in the three developmental stages.In a small group of cultivars,the DFR transcription level of red flowers was 862 times higher than that of yellow flowers.These data suggested that the flavonoid pathway is interrupted in the later stage due to the low transcriptional level of DFR,which limits the biosynthesis of anthocyanins in yellow flowers.The transcription levels of F3’H and FLS were upregulated from stage 1 to stage 2,while those of CHI and FLS were downregulated from stage 2 to stage 3.In addition,67 MYBs and 44 bHLHs showed similar transcription profiles with different members of FSGs.The results deepen our understanding of the molecular mechanism of yellow pigment accumulation in tree peony.展开更多
Coronavirus disease 2019(COVID-19)is an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.It is highly contagious and can cause death in severe cases.A...Coronavirus disease 2019(COVID-19)is an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.It is highly contagious and can cause death in severe cases.As reported by the World Health Organization(WHO),as of 6:36 pm Central European Summer Time(CEST),12 August 2022,there had been 585950285 confirmed cases of COVID-19,including 6425422 deaths(WHO,2022).According to previous studies,healthcare workers(HCWs)have a higher risk of developing COVID-19 compared with the general community,and the severity of COVID-19 is closely related to the health-related effects after discharge(Huang et al.,2021).展开更多
基金funded by Henan Province Science and Technology Breakthrough Project(212102110015)and the Key Scientific Research Projects of Colleges and Universities in Henan Province(21A180018).
文摘Tree peony cultivars with yellow flowers are rare and valuable,but the molecular mechanism of pigment accumulation is not clear.In this study,the petal transcriptome of three developmental stages were sequenced to determine the differentially expressed genes(DEGs)regulating yellow flowers color.The results showed that 10,842 and 12,022 DEGs were screened in stage 1 vs.stage 2 and in stage 2 vs.stage 3,respectively.Through analysis of flavonoid structural genes(FSGs),we found that the transcription level of DFR was very low in the three developmental stages.In a small group of cultivars,the DFR transcription level of red flowers was 862 times higher than that of yellow flowers.These data suggested that the flavonoid pathway is interrupted in the later stage due to the low transcriptional level of DFR,which limits the biosynthesis of anthocyanins in yellow flowers.The transcription levels of F3’H and FLS were upregulated from stage 1 to stage 2,while those of CHI and FLS were downregulated from stage 2 to stage 3.In addition,67 MYBs and 44 bHLHs showed similar transcription profiles with different members of FSGs.The results deepen our understanding of the molecular mechanism of yellow pigment accumulation in tree peony.
基金supported by the Rehabilitation Care Project for Medical Staff Infected with COVID-19 in China launching by Chinese Academy of Engineering and Tencent Foundation.
文摘Coronavirus disease 2019(COVID-19)is an acute respiratory infectious disease caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection.It is highly contagious and can cause death in severe cases.As reported by the World Health Organization(WHO),as of 6:36 pm Central European Summer Time(CEST),12 August 2022,there had been 585950285 confirmed cases of COVID-19,including 6425422 deaths(WHO,2022).According to previous studies,healthcare workers(HCWs)have a higher risk of developing COVID-19 compared with the general community,and the severity of COVID-19 is closely related to the health-related effects after discharge(Huang et al.,2021).
