Effect of Huanglian Jiedu Decoction on the Behavior of Zebrafish with Alzheimer's Disease

[Objectives]To study the effect of Huanglian Jiedu Decoction on the behavior of zebrafish with Alzheimer's disease caused by AlCl 3.[Methods]Each portion of Huanglian Jiedu Decoction was prepared according to the proportion of Coptis chinensis∶Phellodendron chinense Rupr.∶Scutellaria baicalensis Georgi∶Gardenia jasminoides Ellis=63 g∶42 g∶42 g∶63 g.After that,each portion of Huanglian Jiedu Decoction was soaked in 6.3 L water for 30 min and boiled twice at 100℃.The extracts were combined twice and filtered,then concentrated to 308 g/L and put into refrigerator for later use.Before training,zebrafishes were put into T-maze to adapt for 2 d,and then behavioral training was carried out for 7 d.After video recording,the behavior of zebrafish was analyzed by Smart 3.0,and qualified zebrafishes were selected for follow-up experiments.Then 60 successfully trained zebrafishes were randomly divided into control group,model group,positive group,low-dose group,medium-dose group and high-dose group of Huanglian Jiedu Decoction.Except for the control group,all the other groups were exposed to 100μg/L AlCl 3 for 7 d.After that,video was recorded,and behavioral analysis was carried out with behavioral record and analysis software Smart 3.0.And then the zebrafishes in the other four groups except the model group were treated with Huperzine A(4μg/L)and Huanglian Jiedu Decoction(154,308,616 mg/L)for 6 d,respectively.After that,it was recorded and the behavior of each group was analyzed.[Results]There was a significant difference in the time spent in the left area and the percentage of time in the left area between the control group and the model group(P<0.001).The time spent in the left area and the percentage of time in the left area in the model group and positive group,low,medium and high dose groups of Huanglian Jiedu Decoction decreased significantly(P<0.05,P<0.01,P<0.001).The swimming distance in the left area and the percentage of swimming distance in the left area in the model group were significantly higher than those in the control group(P<0.001).There was a significant difference in swimming distance between model group and positive group,low,medium and high dose groups of Huanglian Jiedu Decoction(P<0.01,P<0.001).In the percentage of swimming distance in the left area,there was a significant difference between the model group and the low and high dose groups of Huanglian Jiedu Decoction(P<0.01,P<0.001).[Conclusions]Huanglian Jiedu Decoction can improve the behavior of zebrafish with Alzheimer's disease.

Casein kinase-2 inhibition promotes retinal ganglion cell survival after acute intraocular pressure elevation

Intraocular pressure elevation can induce retinal ganglion cell death and is a clinically reversible risk factor for glaucoma,the leading cause of irreversible blindness.We previously demonstrated that casein kinase-2 inhibition can promote retinal ganglion cell survival and axonal regeneration in rats after optic nerve injury.To investigate the underlying mechanism,in the current study we increased the intraocular pressure of adult rats to 75 mmHg for 2 hours and then administered a casein kinase-2 inhibitor(4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole)by intravitreal injection.We found that intravitreal injection of 4,5,6,7-tetrabromo-2-azabenzimidazole or 2-dimethylamino-4,5,6,7-tetrabromo-1H-benzimidazole promoted retinal ganglion cell survival and reduced the number of infiltrating macrophages.Transcriptomic analysis showed that the mitogen activated protein kinase signaling pathway was involved in the response to intraocular pressure elevation but was not modulated by the casein kinase-2 inhibitors.Furthermore,casein kinase-2 inhibition downregulated the expression of genes(Cck,Htrsa,Nef1,Htrlb,Prph,Chat,Slc18a3,Slc5a7,Scn1b,Crybb2,Tsga10ip,and Vstm21)involved in intraocular pressure elevation.Our data indicate that inhibition of casein kinase-2 can enhance retinal ganglion cell survival in rats after acute intraocular pressure elevation via macrophage inactivation.

Effect of overheating-induced minor addition on Zr-based metallic glasses

Melt treatment is well known to have an important influence on the properties of metallic glasses(MGs).However,for the MGs quenched from different melt temperatures with a quartz tube,the underlying physical origin responsible for the variation of properties remains poorly understood.In the present work,we systematically studied the influence of melt treatment on the thermal properties of a Zr50Cu36Al14 glass-forming alloy and unveiled the microscopic origins.Specifically,we quenched the melt at different temperatures ranging from 1.1Tl to 1.5Tl(Tl is the liquidus temperature)to obtain melt-spun MG ribbons and investigated the variation of thermal properties of the MGs upon heating.We found that glass transition temperature,Tg,increases by as much as 36 K,and the supercooled liquid region disappears in the curve of differential scanning calorimetry when the melt is quenched at a high temperature up to 1.5Tl.The careful chemical analyses indicate that the change in glass transition behavior originates from the incorporation of oxygen and silicon in the molten alloys.The incorporated oxygen and silicon can both enhance the interactions between atoms,which renders the cooperative rearrangements of atoms difficult,and thus enhances the kinetic stability of the MGs.

Magnetic diagnostics layout design for CFETR plasma equilibrium reconstruction

Plasma equilibrium reconstruction provides essential information for tokamak operation and physical analysis.An extensive and reliable set of magnetic diagnostics is required to obtain accurate plasma equilibrium.This study designs and optimizes the magnetic diagnostics layout for the reconstruction of the equilibrium of the plasma according to the scientific objectives,engineering design parameters,and limitations of the Chinese Fusion Engineering Test Reactor(CFETR).Based on the CFETR discharge simulation,magnetic measurement data are employed to reconstruct consistent plasma equilibrium parameters,and magnetic diagnostics'number and position are optimized by truncated Singular value decomposition,verifying the redundancy reliability of the magnetic diagnostics layout design.This provides a design solution for the layout of the magnetic diagnostics system required to control the plasma equilibrium of CFETR,and the developed design and optimization method can provide effective support to design magnetic diagnostics systems for future magnetic confinement fusion devices.

软土地区河道开挖对下方地铁隧道变形的影响及其控制措施

[目的]地铁盾构隧道因上方河道开挖卸荷产生隆起变形,可能会影响地铁隧道的正常运行。为此,应就软土地区河道开挖工程对下方地铁隧道的变形影响进行研究,并制定地铁隧道变形控制措施。[方法]介绍了案例工程的概况,采用有限元方法对其实测数据进行分析,验证了土体本构模型及参数的合理性。基于具有典型软土特征的宁波地区地层剖面和土层参数,建立了土体简化模型,依次分析了河道开挖宽度变化对下方地铁隧道变形的影响、河道开挖深度变化对下方地铁隧道变形的影响、软土层埋深对地铁隧道变形的影响。介绍了门架式土体加固措施及门架式梁板加固措施两种变形控制措施,将考虑了控制变形措施的计算模型与基准模型的有限元计算结果进行了比较分析。[结果及结论]在土体加固和河道开挖阶段,下方地铁隧道变形分别表现为沉降和隆起;河道开挖断面面积相同时,减小河道的开挖深度、增加河道开挖宽度,有利于减少下方地铁隧道变形;地铁隧道竖向位移主要由其下方土体回弹变形产生;门架式梁板加固措施对于地铁隧道隆起变形的控制效果明显。

高端车桥用水性双组分环氧底面合一涂料的制备与研究

研制了一种高端车桥用水性双组分环氧底面合一涂料,研究了主要成膜物、防锈颜料、流变助剂和消泡剂对涂料性能和施工性的影响。结果表明,环氧乳液选用AQUAER-3015和3EE102W复配,按照3:1复配,固化剂选用硬度高、耐水好的AQUAEPO-3126,n(环氧)∶n(活泼氢)=1∶0.8,防锈颜料选择GA-4、WJ1711和APW-Ⅱ复配,流变助剂选择DeuRheo WT-102、HM-30S、Bentone LT和SCM-198复配,质量比为3∶3∶3∶5,消泡剂选择HOS 401W、HOS 420和HOS 402W复配,质量比为1∶2∶3时,该水性双组分环氧底面合一涂料的性能最优,可以适用现场施工工艺条件。

水性双组分底面合一防腐蚀涂料的研究

介绍一种能兼顾耐腐蚀性与耐老化性能的水性双组分底面合一防腐蚀涂料新体系,阐述其制备工艺并探讨了水性羟丙树脂、异氰酸酯固化剂、防锈颜料、涂料体系的颜基比(P/B)和膜厚等变化对水性双组分底面合一防腐蚀涂料的涂膜外观及性能的影响。最终实现“1C1B”工艺施工,单道涂层膜厚≥45μm,耐盐雾性能可达到360 h,氙灯耐老化性能达到600 h。

一种L波段国产GaN固态雷达发射机

为了应对国产雷达所有元器件均国产化需求,设计了一款工作频点在1.2GHZ~1.4 GHz的国产氮化镓(gallium nitride,GaN)固态雷达发射机。使用国产GaN功率模块对发射系统最大限度地进行小型化设计,降低组件的重量,加强设备的高可靠性及维护方便性设计,进一步完善GaN功率模块在线测试功能,使产品具有高可靠性和完善的雷达故障诊断功能。增加系统的监控、检测及显示功能,并将各种工作状态信号都传输到主监控上,使系统的各种工作状态都能够在终端上显示,使操作人员通过终端界面便可以检查发射系统的各种工作参数,判断发射机的工作状态。在电磁兼容方面通过谐波滤波器对二次谐波的抑制,测试结果表明,发射系统输出信号的二次谐波小于等于-66dB。

The effect of adjuvant transarterial chemoembolization for hepatocellular carcinoma after liver resection based on risk stratification

Background:There is currently no standard adjuvant treatment proven to prevent hepatocellular carcinoma(HCC)recurrence.Recent studies suggest that postoperative adjuvant transarterial chemoembolization(PA-TACE)is beneficial for patients at high risk of tumor recurrence.However,it is difficult to select the patients.The present study aimed to develop an easy-to-use score to identify these patients.Methods:A total of 4530 patients undergoing liver resection were recruited.Independent risk factors were identified by Cox regression model in the training cohort and the Primary liver cancer big data transarterial chemoembolization(PDTE)scoring system was established.Results:The scoring system was composed of ten risk factors including alpha-fetoprotein(AFP),albuminbilirubin(ALBI)grade,operative bleeding loss,resection margin,tumor capsular,satellite nodules,tumor size and number,and microvascular and macrovascular invasion.Using 5 points as risk stratification,the patients with PA-TACE had higher recurrence-free survival(RFS)compared with non-TACE in>5 points group(P<0.001),whereas PA-TACE patients had lower RFS compared with non-TACE in≤5 points group(P=0.013).In the training and validation cohorts,the C-indexes of PDTE scoring system were 0.714[standard errors(SE)=0.010]and 0.716(SE=0.018),respectively.Conclusions:The model is a simple tool to identify PA-TACE for HCC patients after liver resection with a favorable performance.Patients with>5 points may benefit from PA-TACE.

Localized-domains staging structure and evolution in lithiated graphite

Intercalation provides to the host materials a means for controlled variation of many physical/chemical properties and dominates the reactions in metal‐ion batteries.Of particular interest is the graphite intercalation compounds with intriguing staging structures,which however are still unclear,especially in their nanostructure and dynamic transition mechanism.Herein,the nature of the staging structure and evolution of the lithium(Li)‐intercalated graphite was revealed by cryogenic‐transmission electron microscopy and other methods at the nanoscale.The intercalated Li‐ions distribute unevenly,generating local stress and dislocations in the graphitic structure.Each staging compound is found macroscopically ordered but microscopically inhomogeneous,exhibiting a localized‐domains structural model.Our findings uncover the correlation between the long‐range ordered structure and short‐range domains,refresh the insights on the staging structure and transition of Li‐intercalated/deintercalated graphite,and provide effective ways to enhance the reaction kinetic in rechargeable batteries by defect engineering.

三级公立医院绩效考核中住院病案首页存在的问题及改进对策

随着医改的持续深入,公立医院绩效考核成为指引公立医院高质量发展的重要抓手。在公立医院绩效考核中,一些指标涉及住院病案首页信息,切实抓好和提升住院病案首页质量,可有效提升公立医院绩效考核成绩。在工作中,住院病案首页存在一些问题,影响到三级公立医院绩效考核结果,宜针对具体问题采取有效应对策略。这对提升医院医疗质量,改善医院管理水平,提高公立医院绩效考核成绩有重要的意义。

连锁桩墙在软土地区深基坑工程中的应用

连锁混凝土预制桩墙是一种兼具挡土和止水功能的创新基坑支护结构。依托宁波软土地区某深基坑工程,总结了连锁混凝土预制桩墙在设计与施工方面的难点与关键技术,并在施工便利性与环保性方面将其与传统钻孔灌注桩进行了对比。结果表明:连锁混凝土预制桩墙具有安全、高效和环保等优点,适用于软土地区基坑工程,是一种值得推广的绿色基坑支护技术。

Microneedles for Enhanced Topical Treatment of Skin Disorders:Applications,Challenges,and Prospects

Microneedles(MNs)can be used for the topical treatment of skin disorders as they directly deliver therapeutics to the site of skin lesions,resulting in increased therapeutic efficacy while having minimum side effects.MNs are used to deliver different kinds of therapeutics(e.g.,small molecules,macromolecules,nanomedicines,living cells,bacteria,and exosomes)for treating various skin disorders,including superficial tumors,wounds,skin infections,inflammatory skin diseases,and abnormal skin appearance.The therapeutic efficacy of MNs can be improved by integrating the advantages of multiple therapeutics to perform combination therapy.Through careful designing,MNs can be further modified with biomimetic structures for the responsive drug release from internal and external stimuli and to enhance the transdermal delivery efficiency for robust therapeutic outcomes.Some studies have proposed the use of drug-free MNs as a promising mechanotherapeutic strategy to promote wound healing,scar removal,and hair regeneration via a mechanical communication pathway.Although MNs have several advantages,the practical application of MNs suffers from problems related to industrial manufacture and clinical evaluation,making it difficult for clinical translation.In this study,we summarized the various applications,emerging challenges,and developmental prospects of MNs in skin disorders to provide information on ways to advance clinical translation.

Prediction of multifaceted asymmetric radiation from the edge movement in density-limit disruptive plasmas on Experimental Advanced Superconducting Tokamak using random forest

Multifaceted asymmetric radiation from the edge(MARFE) movement which can cause density limit disruption is often encountered during high density operation on many tokamaks. Therefore, identifying and predicting MARFE movement is meaningful to mitigate or avoid density limit disruption for the steady-state high-density plasma operation. A machine learning method named random forest(RF) has been used to predict the MARFE movement based on the density ramp-up experiment in the 2022’s first campaign of Experimental Advanced Superconducting Tokamak(EAST). The RF model shows that besides Greenwald fraction which is the ratio of plasma density and Greenwald density limit, dβp/dt,H98and d Wmhd/dt are relatively important parameters for MARFE-movement prediction. Applying the RF model on test discharges, the test results show that the successful alarm rate for MARFE movement causing density limit disruption reaches ~ 85% with a minimum alarm time of ~ 40 ms and mean alarm time of ~ 700 ms. At the same time, the false alarm rate for non-disruptive and non-density-limit disruptive discharges can be kept below 5%. These results provide a reference to the prediction of MARFE movement in high density plasmas, which can help the avoidance or mitigation of density limit disruption in future fusion reactors.

Development of an upgraded motional Stark effect diagnostic system on EAST tokamak

A new multi-channel motional Stark effect(MSE)diagnostic system has been developed on the upgraded EAST tokamak,which was installed on the port C to observe a tangential neutral beam.A telecentric imaging lens was deployed to ensure uniform illumination from the core to the boundary.A square fiber head which contained 23 fiber bundles was mounted to this imaging lens;each fiber bundle contained 19 fibers and two of them were assigned to CXRS and BES spectrometer,respectively.The angle tuning method was used for matching the Doppler shift of theσcomponent’s wavelength which was caused by the beam voltage.At the present stage,the MSE system only contains ten channels that would be extended to 23 channels in the future,covering a measurement range from R=1.8 to R=2.27 m with a temporal resolution of 10 ms and a spatial resolution of3 cm.The polarization angle-constrained q profiles and current density profiles were reconstructed with EFIT equilibrium reconstructions.In the sawtooth discharges,the q=1 surface position was validated by the ECE signals,which further verified the rationality of the MSE measurement.

A combined magnetic field stabilization system for improving the stability of 40Ca^(+) optical clock

Future applications of portable40Ca^(+)optical clocks require reliable magnetic field stabilization to improve frequency stability, which can be achieved by implementing an active and passive magnetic field noise suppression system. On the one hand, we have optimized the magnetic shielding performance of the portable optical clock by reducing its apertures and optimizing its geometry;on the other hand, we have introduced an active magnetic field noise suppression system to further suppress the magnetic field noise experienced by the ions. These efforts reduced the ambient magnetic field noise by about 10000 times, significantly reduced the linewidth of the clock transition spectrum, improved the stability of the portable40Ca+optical clock, and created the conditions for using portable optical clocks in non-laboratory magnetic field environments. This active magnetic field suppression scheme has the advantages of simple installation and wide applicability.

A cryogenic radio-frequency ion trap for a 40Ca^(+) optical clock

A liquid-nitrogen cryogenic40Ca^(+)optical clock is presented that is designed to greatly reduce the blackbody radiation(BBR) shift. The ion trap, the electrodes and the in-vacuum BBR shield are installed under the liquid-nitrogen container,keeping the ions in a cryogenic environment at liquid-nitrogen temperature. Compared with the first design in our previous work, many improvements have been made to increase the performance. The liquid-nitrogen maintenance time has been increased by about three times by increasing the volume of the liquid-nitrogen container;the trap position recovery time after refilling the liquid-nitrogen container has been decreased more than three times by using a better fixation scheme in the liquid-nitrogen container;and the magnetic field noise felt by the ions has been decreased more than three times by a better design of the magnetic shielding system. These optimizations make the scheme for reducing the BBR shift uncertainty of liquid-nitrogen-cooled optical clocks more mature and stable, and develop a stable lock with a narrower linewidth spectrum,which would be very beneficial for further reducing the overall systematic uncertainty of optical clocks.

Isotope-Shift Measurement of Bosonic Yb^(+)Ions

We present a method that the atomic transition frequency measurement relies on the accurate wavemeter,optical frequency comb and stable Fabry–Pérot cavity to precise determination of stable even isotope shift on single Yb+ion(A=168,170,172,174,176).The 6s^(2)S_(1/2)←→6p^(2)P_(1/2)and 5d^(2)D_(3/2)←→6s^(3)[3/2]_(1/2)resonance dipole transition frequencies are preliminarily measured by using a wavemeter which is calibrated by the 729 nm clock laser of ^(40)Ca^(+).Meanwhile,those frequencies are double checked by using optical frequency comb for correction of deviation.Ultimately,by changing frequency locking points at an ultralow expansion cavity more slightly and monitoring the corresponding atomic fluorescence changing with 17%,we finally improve the resonant frequency uncertainty to±6 MHz,which is one order of improvement in precision higher than previously published measurements on the same transitions.A King-plot analysis with sensitivity to coupling between electrons and neutrons is carried out to determine the field and mass shift constants.Our measurement combined with existing or future isotope shift measurements can be used to determine basic properties of atomic nuclei,and to test new forces beyond the Standard Model.

一种轮毂用水性烤漆的研制

介绍了一种轮毂用水性烤漆的研制方法及其涂层性能。采用控制变量法,研究分析了不同主体树脂组合、氨基树脂用量、流平剂和消泡剂的选择、催化剂用量以及增稠剂的筛选与复配对产品性能的影响,从而优选出合适的原材料及最佳的组合和用量。对水性烤漆进行了性能评估,该水性烤漆具有在阳离子电泳漆上的附着性好、重涂性好、物化性能好的特点,现已被应用于钢/铝轮毂、汽车五金配件、电梯板材等产品的涂覆中。

透皮给药实心微针阵列的紫外压印工艺方法

文中提出了一种紫外压印技术成型透皮给药实心微针的工艺方法,以C-coating光刻胶作为微针成型的原材料,采用微机电技术加工的聚二甲基硅氧烷(PDMS)微针模具,探究了紫外压印实验中影响微针成型质量的工艺条件。实验结果表明,利用C-coating光刻胶抽真空12 min以上,紫外曝光170~200 s时,制备的紫外压印微针的高度能达到230μm。通过纳米压痕力学测试和猪皮离体皮肤穿刺实验,证明这种紫外压印微针的针尖在承受50 mN的力时没有发生断裂破坏现象,并且能够顺利刺入猪皮达到良好的透皮给药效果,为紫外压印技术成型透皮给药微针提供了一定的技术参考。