苏云金芽胞杆菌XL6 BTXL6＿11095基因对生物被膜相关表型的调控

在细菌生物被膜(bacterial biofilm, BBF)状态下,细菌往往具有更强的抗紫外线能力、环境适应性和耐药性。本课题组在前期工作中通过转座子插入突变强生物被膜产生菌苏云金芽胞杆菌(Bacillus thuringiensis,Bt)XL6,筛选出了一个可能调控生物被膜的基因BTXL6_11095。本文以该基因为研究对象,通过生物信息学手段分析该基因功能,构建了BTXL6_11095基因敲除菌株,并对其生物被膜相关表型进行了分析。生物信息学分析结果表明BTXL6_11095基因的结构域为PAS-GGDEF-GGDEF-EAL。生物被膜相关表型分析表明,与野生菌株相比,基因敲除菌株的生长曲线基本不变,群游能力上升,生物被膜形成能力减弱、UV-B抗性降低。本研究通过对BTXL6_11095基因表型变化综合评估,从功能角度揭示此基因对Bt XL6生物被膜相关表型的影响,为进一步解析Bt XL6生物被膜调控网络和构建高抗UV的工程生物被膜奠定了基础。

Building New Bridges of International Friendship and Making New Achievements for the Belt and Road——A factual report of foreign aid projects of Beijing Xingyou Engineering Project Management Co., Ltd.

At 09:15 local time on May 13,2018 in the Republic of Mozambique,Li Zhanshu,a member of the Standing Committee of the Political Bureau of the CPC Central Committee and Chairman of the Standing Committee of the National People's Congress (NPC),and Veronica Macamo,Speaker of the Republic of Mozambique,attended the founding ceremony of the Chinese aid project for the Confucius Institute and Media Arts Institute in Mozambique.