Analysis of influencing factors of anxiety and depression in patients with periodontitis

BACKGROUND Periodontitis is a chronic oral disease caused by pathogenic microorganisms that corrode tooth tissue,form periodontal pockets,absorb alveolar bone,and finally lead to tooth loss.During treatment,patients are prone to anxiety,tension,and other negative emotions,which affect their ability to face the disease and may also lead to aggravation of the original condition and affect oral health.Therefore,it is important to improve the negative psychology of patients with periodontitis to clarify the factors that may lead to negative psychological emotions.AIM To investigate the risk factors that may lead to anxiety and depression in patients with periodontitis.METHODS One hundred patients with periodontitis were selected between March 2022 and March 2023 at our hospital.All patients were assessed with the Zung Self-rating Depression Scale(SDS)(≥53 points indicate a depressive state)and Zung Selfrating Anxiety Scale(SAS)(≥50 points indicates an anxious state).In this study,patients who experienced anxiety or depression were included in the occurrence group and those without anxiety or depression were included in the nonoccurrence group.The baseline data of the two groups were compared to explore the risk factors for anxiety and depression in patients with periodontitis.RESULTS A total of 100 patients with periodontitis were included in this study.According to the SDS,38 patients(38.00%)developed depression,with an average SDS score of(68.52±5.85)points.According to the SAS,40 patients(40.00%)developed anxiety,and the average SAS score was(72.15±4.15)points.In this study,56 patients with anxiety or depression were included.Compared with the nonoccurrence group,the occurrence group had higher ages(≥60 years),lower level of hope(low level),educational level(high school or below),disease perception(poor),and sleep disorder(yes).The negative coping dimension scores of the simplified coping style questionnaire(SCSQ)and Dental Fear Scale(DFS)in the occurrence group were higher,whereas the score of the positive coping dimension of the SCSQ was significantly lower(P<0.05).There were no significant differences in the other data between the groups(P>0.05).The results of multiple logistics regression analysis showed that age(≥60 years),level of hope(low level),educational level(high school or below),disease perception(poor),sleep disorder(yes),high negative coping dimension scores of SCSQ,high score of DFS,and low positive coping dimension scores of SCSQ were all factors contributing to the anxiety and depression in patients with periodontitis(odds ratio>1,P<0.05).CONCLUSION Age,hope level,educational level,disease perception,sleep disorders,coping style,and dental fear were all associated with anxiety and depression in patients with periodontitis.

固压柱塞泵轴瓦工装技术设计与应用

在油气开采领域,固井压裂柱塞泵工作的可靠性与稳定性对油气开采效率具有决定性影响。轴瓦是固井与压裂柱塞泵的核心机械部件,负责确保曲轴与连杆之间的稳定可靠连接。本研究针对轴瓦制造过程中的关键工艺工装进行了结构创新设计,所设计的轴瓦工装采用工装座与工装盖的组合结构,通过销钉和螺纹的嵌合连接进行定位,实现了轴瓦的整体安装与加工,显著提升了轴瓦的加工精度、效率和可靠性。轴瓦工装已在实际生产中得到应用验证,验证了其设计结构的有效性和实用性。

电驱压裂装备整体供电技术方案分析及应用

纵观压裂装备发展趋势,电驱压裂装备作为清洁、高效、节能的油气开发装备,势必将逐步取代传统的柴油机驱动压裂装备。结合页岩气压裂作业运行模式及装备配置需求,重点论述燃气涡轮发电机组供电与网电供电两种技术方案,确定了两种供电方案所需的核心装备及整体配置需求,对两种供电方案的燃料成本进行测算与对比,供客户根据实际需求进行选择。整体供电技术方案的研究将为电驱压裂装备的市场开拓、工业化应用及为客户提供更好的作业服务具有重要价值和实际意义。

电驱混砂装置技术方案设计分析

传统混砂装置由柴油发动机驱动液压系统,电控液完成压裂混砂工艺,噪声大、排放高、效率低、控制系统复杂且压裂液密度、添加剂比例等作业参数的控制精度低,对施工作业的质量和可靠性影响较大。笔者重点阐述变频电机组驱动技术方案,通过一组变频电机直接驱动吸入、排出、搅拌、输砂及添加剂机械装置,对电机转速进行变频矢量控制,达到压裂基液、支撑剂及添加剂的高质量混配。此技术方案的实施对大排量混砂装置实现节能减排、降低生产成本及提升作业效率与作业质量具有重要实际意义。

电驱压裂橇研制关键技术研究及应用

电驱压裂装备将替代传统柴油机驱动压裂装备成为非常规油气增产增储作业主力装备。针对采油压驱注水工艺技术作业模式及装备输出能力需求特点,确定2500型电驱压裂橇的技术设计参数,重点对产品实现机理、功能单元组成及研制关键技术开展设计分析研究,构建产品技术方案,通过样机研制、场内试验及工业性试验对产品性能、可靠性及稳定性进行有效验证,产品在华东油田压驱注水工艺技术中得到推广应用。该产品的研制成功,将对推进采油压驱注水工艺技术作业向高效率、低成本、绿色环保方向发展具有重要价值与实际意义。

Extracellular vesicle-mediated heterogeneous communication between cancer and the lymphatic system facilitates lymphatic metastasis

Introduction The dissemination of cancer cells to organs initiates the formation of an aggressive cancer phenotype and is a predominant cause of cancer-associated death.For most epithelial cancers,lymphatic system metastasis has been characterized as the most common and earliest metastatic pathway,and the detection of metastasis in lymph nodes(LNs)often predicts poor survival among patients'.Although increasing attention is being paid to the clinical importance of LN metastasis,the underlying mechanisms have remained unclear in the past decade.Accumulating evidence suggests that the occurrence of LN metastasis is not stochastic but is a programming biological event regulated by the bidirectional crosstalk between metastasis-initiating cancer cells and the tumor microenvironment(TME)2.However,the regulators and patterns of cancer-TME communication in LN metastasis remain to be furtherexplored.

压裂装备智能化作业管理系统设计应用

压裂装备作为非常规油气开发的关键设备,装备的自动化、智能化水平决定作业的高效性和安全性。针对传统压裂装备作业中存在无法远程实时查询设备现场运行实时信息、关键信息数据量大、维保和故障维修针对性不强、组网方式单一及信息化管理水平低等现状,将数据采集技术、5G无线通讯技术、数据可视化技术、AR远程运维技术应用于压裂装备作业管理系统,并对所设计的系统进行应用验证,可达到提升设备整体智能化管理水平和作业安全性的目标,对于提升压裂作业装备的高端化、智能化、绿色化具有重要价值与现实意义。

An HGF-dependent positive feedback loop between bladder cancer cells and fibroblasts mediates lymphangiogenesis and lymphatic metastasis

Background Cancer-associated fibroblasts (CAFs) play a vital role in facilitating tumor progression through extensive reciprocal interplay with cancer cells. Tumor-derived extracellular vesicles (EVs) are the critical mediators involved in the crosstalk between cancer cells and stromal cells, contributing to the metastasis of cancers. Yet, the biological mechanisms of tumor-derived EVs in triggering CAFs phenotype to stimulate the lymph node (LN) metastasis of bladder cancer (BCa) are largely unknown. Here, we aimed to explore the effects and molecular mechanisms of tumor-derived EV-mediated CAFs phenotype in regulating BCa LN metastasis. Methods The high-throughput sequencing was utilized to identify the crucial long non-coding RNA (lncRNA) associated with CAF enrichment in BCa. The functional role of the transition of fibroblasts to CAFs induced by LINC00665-mediated EVs was investigated through the in vitro and in vivo assays. Chromatin isolation by RNA purification assays, fluorescence resonance energy transfer assays, cytokine profiling and patient-derived xenograft (PDX) model were performed to explore the underlying mechanism of LINC00665 in the LN metastasis of BCa. Results We found that CAFs are widely enriched in the tumor microenvironment of BCa, which correlated with BCa lymphangiogenesis and LN metastasis. We then identified a CAF-associated long non-coding RNA, LINC00665, which acted as a crucial mediator of CAF infiltration in BCa. Clinically, LINC00665 was associated with LN metastasis and poor prognosis in patients with BCa. Mechanistically, LINC00665 transcriptionally upregulated RAB27B expression and induced H3K4me3 modification on the promoter of RAB27B through the recruitment of hnRNPL. Moreover, RAB27B-induced EVs secretion endowed fibroblasts with the CAF phenotype, which reciprocally induced LINC00665 overexpression to form a RAB27B-HGF-c-Myc positive feedback loop, enhancing the lymphangiogenesis and LN metastasis of BCa. Importantly, we demonstrated that blocking EV-transmitted LINC00665 or HGF broke this loop and impaired BCa lymphangiogenesis in a PDX model. Conclusion Our study uncovers a precise mechanism that LINC00665 sustains BCa LN metastasis by inducing a RAB27B-HGF-c-Myc positive feedback loop between BCa cells and fibroblasts, suggesting that LINC00665 could be a promising therapeutic target for patients with LN metastatic BCa.

Implantable optical fiber microelectrode with anti-biofouling ability for in vivo photoelectrochemical analysis

In-situ monitoring of neurochemicals is of vital importance for the understanding of brain functions.Microelectrode-based photoelectrochemical(PEC) sensing has emerged as a promising tool for in vivo analysis since it inherits the merits of both optical and electrochemical methods. However, the in-situ excitation of photoactive materials on the photoelectrode in living body is still a challenge because of limited tissue penetration depth of light. To circumvent this problem, we herein developed an implantable optical fiber(OF)-based microelectrode for in vivo PEC analysis. The working electrode was constructed by coating Au film as conducting layer and CdS@ZnO as photoactive material on a micron-sized OF,which was free of the limitation of light penetration in biological tissues. Further decoration of an antibiofouling layer on the surface made the sensor robust in biosamples. It was successfully applied for monitoring Cu^(2+) level in three different brain regions in the rat model of cerebral ischemia/reperfusion.

Variation of satellite transponder delay

For precise orbit determination of geosynchronous earth orbit(GEO)satellites using transfer ranging observations,it is generally assumed that the variation of the satellite transponder delay is very small and that it can be solved as a constant parameter together with satellite orbit parameters.However,this assumption is too general and it reduces the accuracy of orbit determination for GEO satellites.To study and analyse the impact of the satellite transponder delay on GEO satellites orbit determination,two schemes were proposed.First,the satellite transponder delay was eliminated by forming single-difference observations between two ground stations;second,the satellite transponder delay was described as a constant parameter.The preliminary results demonstrate a difference of about1–2 m between the two schemes when used for precise orbit determination of GEO satellites.By fixing the GEO satellite orbit and other relevant parameters estimated by single-difference model,we inversed the instantaneous transponder delay from non-difference observation.It was found that the satellite transponder delay has a distinct diurnal variation,with an amplitude of 3–4 m.The findings of this paper are helpful in establishing an accurate model of satellite transponder delay and in improving the accuracy of GEO satellites orbit determinations and predictions.

“Dual-Lock-Dual-Key”Controlled Second Near-Infrared Molecular Probe for Specific Discrimination of Orthotopic Colon Cancer and Imaging-Guided Tumor Excision

Fluorescence imaging in the second infrared window(1000-1700 nm)has emerged as a promising approach to tumor diagnosis.However,the currently available second near-infrared(NIR-II)imaging agents are based on the“always on”modality or single biomarker activation,which are subject to limited imaging contrast,nonspecific response,and even false-positive diagnosis.Here,we developed a H2S/H+dual-stimuli responsive NIR-II fluorescent probe,WH-N3,for precise tumor delimitation and intraoperative fluorescence-guided surgical resection.WH-N3 itself is nonfluorescent,and it can only light up through synergistic activation by H2S and in the tumor acidic environment(TEM).Such a“duallock-dual-key”strategy-based activatable probe exhibited significantly higher tumor-to-normal tissue(T/N)ratios than the“always on”agent(ICG)and single parameter responsive counterpart probes in the imaging of colon tumors,which overexpresses H2S.WH-N3 was also able to visualize the tumor-derived endogenous H2S fluctuation and accurately differentiate tumor types based on H2S content discrepancy.More excitingly,under the guidance of the probe’s highly specific NIR-II fluorescence,a tiny orthotopic colon tumor with diameter down to 0.8 mm was facilely resected.We expect our dual-stimuli responsive strategy will contribute more reliable tools for specific discrimination and imaging-guided excision of tumor.