The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production.In our studies,we found that AtC...The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production.In our studies,we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis.Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis.The expression of some genes responsible for Cd2+ transportation into vacuoles was induced,and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment.The expression of Cd-transportation related genes was not in response to Cd-stress,whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment.The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment.Additionally,malondialdehyde (MDA),hydrogen peroxide,and Cd2+ contents,and activities of some antioxidative enzymes,differed between the wild type and atcsr-2.Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years.The findings revealed that the expression pattern of H2 S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2,and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity.Finally,one possible suggestion was given:down-regulation of atcsr-2,depending on H2S gas-transmitter not only weakened Cd2+ chelation,but also reduced Cd2+ transportation into vacuoles,as well as enhancing the Cd2+ assimilation,thus rendering atcsr-2 mutant sensitive to Cd-stress.展开更多
Antibiotic abuse in food processing could threaten human health via either direct pathogen or drug-resistant infection.To curb this situation,it is of vital significance to identify trace level of antibiotics in foods...Antibiotic abuse in food processing could threaten human health via either direct pathogen or drug-resistant infection.To curb this situation,it is of vital significance to identify trace level of antibiotics in foodstuffin a point-of-care inspection manner.Here,a smartphone-based electrochemical system was developed for the quantitation of chloramphenicol(CAP).The differential pulse voltammetric reduction of CAP was measured facilely on a piece of gold-deposited screen-printed electrode,which was docked onto a palm-sized detector with remote directives from a mobile APP of Bluetooth handshaking and digital display.Under optimal conditions,the target CAP could be determined in a range from 1 nmol/L to 5μmol/L with a detection limit of 0.25 nmol/L.Further tests on emulate samples demonstrated the miniaturized device could handle the food-screening scenarios with accuracy,convenience and quick response.展开更多
基金Project supported by the Research Fund for the Doctoral Program of Higher Education of China (No. 20091401110004)the Science and Technology Special Project of Shanxi Province,China (2012,to QiangZHANG)the Shanxi Scholarship Council of China (No. 2011-007)
文摘The gene AtCSR encodes peptidyl-prolyl cis/trans isomerases (PPIases) that accelerate energetically unfavorable cis/trans isomerization of the peptide bond preceding proline production.In our studies,we found that AtCSR was associated with cadmium (Cd)-sensitive response in Arabidopsis.Our results show that AtCSR expression was triggered by Cd-stress in wild type Arabidopsis.The expression of some genes responsible for Cd2+ transportation into vacuoles was induced,and the expression of the iron-regulated transporter 1 (IRT1) related to Cd2+ absorption from the environment was not induced in wild type with Cd2+ treatment.The expression of Cd-transportation related genes was not in response to Cd-stress,whereas IRT expression increased dramatically in atcsr-2 with Cd2+ treatment.The expression of glutathione 1 (GSH1) was consistent with GSH being much lower in atcsr-2 in comparison with the wild type with Cd2+ treatment.Additionally,malondialdehyde (MDA),hydrogen peroxide,and Cd2+ contents,and activities of some antioxidative enzymes,differed between the wild type and atcsr-2.Hydrogen sulfide (H2S) has been confirmed as the third gas-transmitter over recent years.The findings revealed that the expression pattern of H2 S-releasing related genes and that of Cd-induced chelation and transportation genes matched well in the wild type and atcsr-2,and H2S could regulate the expression of the Cd-induced genes and alleviate Cd-triggered toxicity.Finally,one possible suggestion was given:down-regulation of atcsr-2,depending on H2S gas-transmitter not only weakened Cd2+ chelation,but also reduced Cd2+ transportation into vacuoles,as well as enhancing the Cd2+ assimilation,thus rendering atcsr-2 mutant sensitive to Cd-stress.
基金financially supported by the National Key Research and Development Program of China for International Science&Innovation Cooperation Major Project between Governments(2018YFE0113200)National Natural Science Foundation of China(21874071,22104058)+1 种基金Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_0281)Fundamental Research Funds for the Central Universities(30921013112,30920021125,30922010501)
文摘Antibiotic abuse in food processing could threaten human health via either direct pathogen or drug-resistant infection.To curb this situation,it is of vital significance to identify trace level of antibiotics in foodstuffin a point-of-care inspection manner.Here,a smartphone-based electrochemical system was developed for the quantitation of chloramphenicol(CAP).The differential pulse voltammetric reduction of CAP was measured facilely on a piece of gold-deposited screen-printed electrode,which was docked onto a palm-sized detector with remote directives from a mobile APP of Bluetooth handshaking and digital display.Under optimal conditions,the target CAP could be determined in a range from 1 nmol/L to 5μmol/L with a detection limit of 0.25 nmol/L.Further tests on emulate samples demonstrated the miniaturized device could handle the food-screening scenarios with accuracy,convenience and quick response.
