Fine-grained lacustrine sedimentation controlled by astronomical cycles remains a research weakness in sedimentology studies,as most studies have concentrated on how astronomical cycles affect the normal lacustrine fi...Fine-grained lacustrine sedimentation controlled by astronomical cycles remains a research weakness in sedimentology studies,as most studies have concentrated on how astronomical cycles affect the normal lacustrine fine-grained sedimentation,but how they affect the lacustrine fine-grained event sedimen-tation has been rarely studied.Therefore,this work researched the characteristics of event sedimentation by systematically observing the cores from 30 cored wells in the Shahejie Formation of the Dongying Sag at a depth of over 1800 m,with more than 4000 thin sections being authenticated and over 1000 whole rocks being analyzed by X-ray diffraction(XRD).The research object was the Chunshang Sub-member of Upper Es_(4) in the Fanye 1 well,as it had the most comprehensive analysis data and underwent the most continuous coring.We divided astronomical cycles into different orders and made corresponding curves using the gamma-ray(GR)curve,spectral analysis,power spectrum estimation,and module extreme values,there were 6 long eccentricity periods,22 short eccentricity periods,65.5 obliquity cycles,and 110.5 precession cycles in this sub-member.On this basis,this study analyzed the control of astronomical cycles on the lacustrine fine-grained event sedimentation,and the research shows deposits were developed by slide-slump,turbidities,hyperpycnites,and tempestites in the Chunshang Sub-member of the Upper Es_(4),with higher long eccentricity,the monsoon climate contributes to the formation of storm currents,while with lower long eccentricity,the surface deposits are severely eroded by rivers and rainfalls,thus developing the slide-slump,turbidities,and hyperpycnites.The relationship between the lacustrine fine-grained event sedimentation and astronomical cycles was studied in this case study,which can promote research on fine-grained sedimentary rocks in genetic dynamics and boost the theoretical and disciplinary development in fine-grained sedimentology.展开更多
This paper mainly introduced the preparation of silk fibroin membranes and their structural change characteristics.Silk fibroin membranes can be used as tissue engineering materials,enzyme-immobilizing membranes,biose...This paper mainly introduced the preparation of silk fibroin membranes and their structural change characteristics.Silk fibroin membranes can be used as tissue engineering materials,enzyme-immobilizing membranes,biosensors and drug controlled-release membranes and other different materials.They have excellent characteristics such as non-toxic,non-polluting and degradable,and thus have broad application prospects.展开更多
The planting and afforestation of mulberry and its application as animal feed conform to the spirit that comprehensive utilization of biomass such as agriculture and forestry is the direction of sustainable developmen...The planting and afforestation of mulberry and its application as animal feed conform to the spirit that comprehensive utilization of biomass such as agriculture and forestry is the direction of sustainable development in the National Program for Medium-to-Long-Term Scientific and Technological Development . The application of mulberry as animal feed has attracted international attention, but the cultivar identification of forage mulberry is still controversial in the industry. The paper summarizes the current situation of forage mulberry in animal husbandry, and expounds how to define and classify forage mulberry.展开更多
Silk can promote the penetration of oxygen and water and support the attachment and proliferation of neurons and Schwann cells,and has appropriate biodegradation kinetics and flexible physical strength.It is a high-qu...Silk can promote the penetration of oxygen and water and support the attachment and proliferation of neurons and Schwann cells,and has appropriate biodegradation kinetics and flexible physical strength.It is a high-quality natural tissue engineering material and can be used as an ideal matrix for nerve repair.This paper reviewed the structure and properties of silk and the research progress of silk nerve conduits in peripheral nerve regeneration in recent years,and prospected its clinical application.展开更多
Silk sericin is a natural polymer,which is often discarded as waste in silk reeling production.Sericin protein increases the COD value of degumming wastewater and increases the difficulty of sewage treatment.The recov...Silk sericin is a natural polymer,which is often discarded as waste in silk reeling production.Sericin protein increases the COD value of degumming wastewater and increases the difficulty of sewage treatment.The recovery and utilization of sericin protein can minimize environmental problems,and has high scientific and commercial value.This paper introduced and analyzed current technical methods of recycling sericin from silk degumming wastewater,providing a reference for the utilization of recovered sericin.展开更多
Pleural and peritoneal metastasis accompanied by malignant pleural effusion(MPE)or malignant ascites(MA)is frequent in patients with advanced solid tumors that originate from the lung,breast,gastrointestinal tract and...Pleural and peritoneal metastasis accompanied by malignant pleural effusion(MPE)or malignant ascites(MA)is frequent in patients with advanced solid tumors that originate from the lung,breast,gastrointestinal tract and ovary.Regional delivery of CAR-T cells represents a new strategy to control tumor dissemination in serous cavities.However,malignant effusions constitute an immune-suppressive environment that potentially induces CAR-T cell dysfunction.Here,we demonstrated that the anti-tumor cytotoxicity of conventional 2nd-generation CAR-T cells was significantly inhibited by both the cellular and non-cellular components of MPE/MA,which was primarily attributed to impaired CAR-T cell proliferation and cytokine production in MPE/MA environment.Interestingly,we found that PD-L1 was widely expressed on freshly-isolated MPE/MA cells.Based on this feature,a novel PD-L1-targeting chimeric switch receptor(PD-L1.BB CSR)was designed,which can bind to PD-L1,switching the inhibitory signal into an additional 4-1BB signal.When co-expressed with a 2nd-generation CAR,PD-L1.BB CSR-modified CAR-T cells displayed superior fitness and enhanced functions in both culture medium and MPE/MA environment,causing rapid and durable eradication of pleural and peritoneal metastatic tumors in xenograft models.Further investigations revealed elevated expressions of T-cell activation,proliferation,and cytotoxicity-related genes,and we confirmed that PD-L1 scFv and 4-1BB intracellular domain,the two important components of PD-L1.BB CSR,were both necessary for the functional improvements of CAR-T cells.Overall,our study shed light on the clinical application of PD-L1.BB CSR-modified dual-targeting CAR-T cells.Based on this study,a phase I clinical trial was initiated in patients with pleural or peritoneal metastasis(NCT04684459).展开更多
基金supported by the Study on Astronomical Stratigraphic Period of Lacustrine Shale and High Resolution Sedimentary Cycle in Logging(41872166)of the National Natural Science Foundation of China and the Exploration and Development Research Institute,Shengli Oilfield Company,SINOPEC.
文摘Fine-grained lacustrine sedimentation controlled by astronomical cycles remains a research weakness in sedimentology studies,as most studies have concentrated on how astronomical cycles affect the normal lacustrine fine-grained sedimentation,but how they affect the lacustrine fine-grained event sedimen-tation has been rarely studied.Therefore,this work researched the characteristics of event sedimentation by systematically observing the cores from 30 cored wells in the Shahejie Formation of the Dongying Sag at a depth of over 1800 m,with more than 4000 thin sections being authenticated and over 1000 whole rocks being analyzed by X-ray diffraction(XRD).The research object was the Chunshang Sub-member of Upper Es_(4) in the Fanye 1 well,as it had the most comprehensive analysis data and underwent the most continuous coring.We divided astronomical cycles into different orders and made corresponding curves using the gamma-ray(GR)curve,spectral analysis,power spectrum estimation,and module extreme values,there were 6 long eccentricity periods,22 short eccentricity periods,65.5 obliquity cycles,and 110.5 precession cycles in this sub-member.On this basis,this study analyzed the control of astronomical cycles on the lacustrine fine-grained event sedimentation,and the research shows deposits were developed by slide-slump,turbidities,hyperpycnites,and tempestites in the Chunshang Sub-member of the Upper Es_(4),with higher long eccentricity,the monsoon climate contributes to the formation of storm currents,while with lower long eccentricity,the surface deposits are severely eroded by rivers and rainfalls,thus developing the slide-slump,turbidities,and hyperpycnites.The relationship between the lacustrine fine-grained event sedimentation and astronomical cycles was studied in this case study,which can promote research on fine-grained sedimentary rocks in genetic dynamics and boost the theoretical and disciplinary development in fine-grained sedimentology.
基金Supported by Chongqing Natural Science Foundation Project(18219)Chongqing Scientific Research Institution Performance Incentive Project(19537,20521)+1 种基金Chongqing Rongchang Agriculture and Animal Husbandry High Tech Industry R&D Special Project(20209)Chongqing Modern Mountain Characteristic Benefit Agriculture(Sericulture)Technology System Diversified Development Research Office(18304).
文摘This paper mainly introduced the preparation of silk fibroin membranes and their structural change characteristics.Silk fibroin membranes can be used as tissue engineering materials,enzyme-immobilizing membranes,biosensors and drug controlled-release membranes and other different materials.They have excellent characteristics such as non-toxic,non-polluting and degradable,and thus have broad application prospects.
基金Supported by Development of Low-Immunogenicity&Water-Soluble Silk Fibroin(22541C)Chongqing Scientific Research Institution Performance Incentive Project(22529J,22531J)+1 种基金Chongqing Rongchang Agriculture and Animal Husbandry High-tech Industry R&D Special Project(20209)Chongqing Modern Mountain Characteristic Benefit Agriculture(Sericulture)Technology System Diversified Development Research Office(18304).
文摘The planting and afforestation of mulberry and its application as animal feed conform to the spirit that comprehensive utilization of biomass such as agriculture and forestry is the direction of sustainable development in the National Program for Medium-to-Long-Term Scientific and Technological Development . The application of mulberry as animal feed has attracted international attention, but the cultivar identification of forage mulberry is still controversial in the industry. The paper summarizes the current situation of forage mulberry in animal husbandry, and expounds how to define and classify forage mulberry.
基金Supported by Development of Low-immunogenicity&Water-soluble Silk Fibroin(22541C)Chongqing Scientific Research Institution Performance Incentive Project(22529J and 22531J)Chongqing Rongchang Agriculture and Animal Husbandry High-tech Industry R&D Special Project(20209).
文摘Silk can promote the penetration of oxygen and water and support the attachment and proliferation of neurons and Schwann cells,and has appropriate biodegradation kinetics and flexible physical strength.It is a high-quality natural tissue engineering material and can be used as an ideal matrix for nerve repair.This paper reviewed the structure and properties of silk and the research progress of silk nerve conduits in peripheral nerve regeneration in recent years,and prospected its clinical application.
基金Supported by Chongqing Natural Science Foundation Project(18219)Chongqing Scientific Research Institution Performance Incentive Project(19537,20521)+1 种基金Chongqing Rongchang Agriculture and Animal Husbandry High-tech Industry R&D Special Project(20209)Chongqing Modern Mountain Characteristic Benefit Agriculture(Sericulture)Technology System Diversified Development Research Office(18304).
文摘Silk sericin is a natural polymer,which is often discarded as waste in silk reeling production.Sericin protein increases the COD value of degumming wastewater and increases the difficulty of sewage treatment.The recovery and utilization of sericin protein can minimize environmental problems,and has high scientific and commercial value.This paper introduced and analyzed current technical methods of recycling sericin from silk degumming wastewater,providing a reference for the utilization of recovered sericin.
基金National Science and Technology Major Project(2018ZX09733001-003-004)National Natural Science Foundation of China(81673001 and 81872489).
文摘Pleural and peritoneal metastasis accompanied by malignant pleural effusion(MPE)or malignant ascites(MA)is frequent in patients with advanced solid tumors that originate from the lung,breast,gastrointestinal tract and ovary.Regional delivery of CAR-T cells represents a new strategy to control tumor dissemination in serous cavities.However,malignant effusions constitute an immune-suppressive environment that potentially induces CAR-T cell dysfunction.Here,we demonstrated that the anti-tumor cytotoxicity of conventional 2nd-generation CAR-T cells was significantly inhibited by both the cellular and non-cellular components of MPE/MA,which was primarily attributed to impaired CAR-T cell proliferation and cytokine production in MPE/MA environment.Interestingly,we found that PD-L1 was widely expressed on freshly-isolated MPE/MA cells.Based on this feature,a novel PD-L1-targeting chimeric switch receptor(PD-L1.BB CSR)was designed,which can bind to PD-L1,switching the inhibitory signal into an additional 4-1BB signal.When co-expressed with a 2nd-generation CAR,PD-L1.BB CSR-modified CAR-T cells displayed superior fitness and enhanced functions in both culture medium and MPE/MA environment,causing rapid and durable eradication of pleural and peritoneal metastatic tumors in xenograft models.Further investigations revealed elevated expressions of T-cell activation,proliferation,and cytotoxicity-related genes,and we confirmed that PD-L1 scFv and 4-1BB intracellular domain,the two important components of PD-L1.BB CSR,were both necessary for the functional improvements of CAR-T cells.Overall,our study shed light on the clinical application of PD-L1.BB CSR-modified dual-targeting CAR-T cells.Based on this study,a phase I clinical trial was initiated in patients with pleural or peritoneal metastasis(NCT04684459).
