Residual antibiotics in food pose a serious long-term threat to human health.Therefore,an on-site visualization method for antibiotic detection is required.However,the requirements of traditional antibiotic testing me...Residual antibiotics in food pose a serious long-term threat to human health.Therefore,an on-site visualization method for antibiotic detection is required.However,the requirements of traditional antibiotic testing methods in terms of operator proficiency and equipment cost hinder the rapid point-of-caretesting detection of suspected samples.Herein,we reported an integrated microfluidic device combining a microfluidic chip containing cruciform valves with immunochromatographic strips for the rapid detection of multiple antibiotics in milk.The rapid qualitative and quantitative analysis of four types of antibiotics(sulfonamides,β-lactams,streptomycin,and tetracyclines)was performed using mobile phone photography and mobile phone application analysis.The detection time was maintained at 10 min.The limits of detection(LODs)for the four antibiotics were 0.15,0.12,0.25,and 0.29 ng/mL,respectively,and the selectivity for the different antibiotics was observed even in a highly complex matrix.This device successfully integrated separation and real-time detection onto a chip and might provide a promising perspective for the detection of multiple antibiotics in milk.展开更多
基金This work was supported financially by the National Natural Science Foundation of China(Nos.82073816 and 21727814)Advanced Talents of Beijing Technology and Business University(No.19008021179).
文摘Residual antibiotics in food pose a serious long-term threat to human health.Therefore,an on-site visualization method for antibiotic detection is required.However,the requirements of traditional antibiotic testing methods in terms of operator proficiency and equipment cost hinder the rapid point-of-caretesting detection of suspected samples.Herein,we reported an integrated microfluidic device combining a microfluidic chip containing cruciform valves with immunochromatographic strips for the rapid detection of multiple antibiotics in milk.The rapid qualitative and quantitative analysis of four types of antibiotics(sulfonamides,β-lactams,streptomycin,and tetracyclines)was performed using mobile phone photography and mobile phone application analysis.The detection time was maintained at 10 min.The limits of detection(LODs)for the four antibiotics were 0.15,0.12,0.25,and 0.29 ng/mL,respectively,and the selectivity for the different antibiotics was observed even in a highly complex matrix.This device successfully integrated separation and real-time detection onto a chip and might provide a promising perspective for the detection of multiple antibiotics in milk.
