CH4 emission rates followed an increased pattern during the growing season at Tibetan Plateau.•Unique genes carried by abundant species were positively correlated with CH4 emission rates.•Climate factors influenced CH...CH4 emission rates followed an increased pattern during the growing season at Tibetan Plateau.•Unique genes carried by abundant species were positively correlated with CH4 emission rates.•Climate factors influenced CH4 emission rates by regulating microbial community and their genes.Microorganisms play pivotal roles in soil methane(CH4)emissions and their functional genes are origins of a key mechanism for soil CH4-cycling.However,understanding of the roles of specific genes(e.g.,unique or shared genes carried by species)underlying CH4-cycling remains elusive.Here,we measured CH4 emission rates and investigated variations in microbial community and the abundance of genes carried by species during the growing season in alpine meadow on the Tibetan Plateau.We discovered that CH4 emission rates increased from 394.4,745.9,and 1092.7µg CH4 m−2 h−1,in April,June,and August,respectively,and had a positive correlation with unique genes carried by abundant species during the growing season.Moreover,we found that unique genes carried by abundant species involved in methanogenesis processes have a higher abundance than methanotrophic processes.Further analysis indicated that climate factors(i.e.,mean monthly temperature(MMT)and mean monthly precipitation(MMP))influenced microbial community and their functional genes,and therefore affected the CH4 emission rates.Overall,the present study provides a novel insight into the variation of soil CH4 emissions from a functional gene perspective,highlighting the important roles of unique genes carried by abundant species in CH4 emissions in the Tibetan Plateau under seasonal variation.展开更多
基金financially supported by the National Natural Science Foundation of China(Grant No.42277284)the 2021 first funds for central government to guide local science and technology development in Qinghai Province(Grant No.2021ZY002)the Second Tibetan Plateau Scientific Expedition and Research Program(Grant Nos.2019QZKK020102,2019OZKK0302).
文摘CH4 emission rates followed an increased pattern during the growing season at Tibetan Plateau.•Unique genes carried by abundant species were positively correlated with CH4 emission rates.•Climate factors influenced CH4 emission rates by regulating microbial community and their genes.Microorganisms play pivotal roles in soil methane(CH4)emissions and their functional genes are origins of a key mechanism for soil CH4-cycling.However,understanding of the roles of specific genes(e.g.,unique or shared genes carried by species)underlying CH4-cycling remains elusive.Here,we measured CH4 emission rates and investigated variations in microbial community and the abundance of genes carried by species during the growing season in alpine meadow on the Tibetan Plateau.We discovered that CH4 emission rates increased from 394.4,745.9,and 1092.7µg CH4 m−2 h−1,in April,June,and August,respectively,and had a positive correlation with unique genes carried by abundant species during the growing season.Moreover,we found that unique genes carried by abundant species involved in methanogenesis processes have a higher abundance than methanotrophic processes.Further analysis indicated that climate factors(i.e.,mean monthly temperature(MMT)and mean monthly precipitation(MMP))influenced microbial community and their functional genes,and therefore affected the CH4 emission rates.Overall,the present study provides a novel insight into the variation of soil CH4 emissions from a functional gene perspective,highlighting the important roles of unique genes carried by abundant species in CH4 emissions in the Tibetan Plateau under seasonal variation.
