Eu-MOF-Based Fluorescent Ratiometric Sensor by Detecting 3,4,5-Trihydroxybenzoic for Fingerprint Visualization on Porous Objects

Latent fingerprints(LFPs)at the crime scene are served as important clues to locate the trajectory of criminal behavior and portray the characteristics of the suspect.Therefore,visualizing LFPs is of considerable significance.In this work,the europium metal-organic framework(Eu-MOF)sensor was successfully constructed for sensitive detection of gallic acid(3,4,5-trihydroxybenzoic acid,GA)and visualization of the sweat LFPs.The boric-acid-modified Eu-MOF was prepared by using the simple one-pot solvothermal method using Eu as the metal i ion center and 3,5-dicarboxybenzeneboronic acid(BBDC)as the organic ligand.The sensor showed desirable photoluminescent performance through the chelating of BBDC with Eu3+.The sensor exhibited the satisfactory linear relationship to GA in the range of 1 nM to 20 nM with a low detection limit of 0.34 nM under the optimized conditions.The prepared sensor with ideal selectivity to GA was successfully applied for visualizing LFPs on porous substrates with the high contrast and superior stability.Given the good performance of the sensor,all fingerprint images obtained from 1200 samples presented clear friction ridges and met the identification criteria.Notably,the sensor had less impact on the subsequent deoxyribonucleic acid(DNA)detection,displaying a promising perspective for applications in extracting physical evidence of site investigation.

N-myc downstream-regulated gene 2 promotes proliferation of HO-8910 ovarian cancer cells

Objective To investigate N-myc downstream-regulated gene 2(NDRG2) expression in ovarian cancer cells and its potential usefulness as a diagnostic marker and/or target for therapeutic intervention.Methods Human NDRG2 L/S gene was obtained by revers-transcription polymerase chain reaction(RT-PCR). Sequence analysis confirmed the identity of NDRG2 L/S gene, which was then inserted into a eukaryotic vector p LNCX2, which was in turn transfected into NDRG2 gene-negative HO-8910 cells. Flow cytometry(FCM) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay were conducted to determine the proliferation rate of HO-8910 cells. Cisplatin resistance of HO-8910 cells transfected with p LNCX2-NDRG2 L/S was evaluated by FCM. Tumors were generated in female nude mice by subcutaneous injection of HO-8910 cells.Results NDRG2 gene was isolated and its expression vector was successfully constructed. NDRG2 expression positively correlated with the proliferation of HO-8910 cells. NDRG2 L/S promoted tumorigenicity in HO-8910 cells.Conclusion The present study identified a novel function of NDRG2 L/S gene and demonstrated its involvement in the promotion of ovarian cancer cell proliferation and enhancement of cisplatin resistance in HO-8910 cells. Future studies are warranted to determine the relationship between NDRG2 upregulation and ovarian cancer progression.

Determination of key components in automobile braking systems based on ABC classification and FMECA

As one of the critical issues in operations management, spare parts planning classification systems based only on cost may not withstand the test of time because of continuing technological advancement or environmental degradation. This study, however, emphasizes using failure mode effect and criticality analysis(FMECA) as a basis for designing an ABC classification system that is capable of more accurately determining critical equipment and making maintenance more efficient. In the proposed methodology, risk priority numbers(RPN) and criticality as an index of safety and reliability can be obtained using a structured failure analysis technique. These indexes can be added to the economic indicator of maintenance cost, and then a classification model of spare parts can be established according to a comprehensive scoring method. The model is calibrated by using case data from a bus braking system, and the components of the braking system are compared and analyzed. The results show that the improved ABC classification method reduces the proportion of key and main components. This method can make maintenance work more efficient, targeting the most critical components, and can reduce administrative costs for enterprises.

Study on Accuracy of Judgments by Chinese Fingerprint Examiners

The interpretation of fingerprint evidence depends on the judgments of fingerprint examiners.This study assessed the accuracy of different judgments made by fingerprint examiners following the Analysis,Comparison,and Evaluation(ACE)process.Each examiner was given five marks for analysis,comparison,and evaluation.We compared the experts’judgments against the ground truth and used an annotation platform to evaluate how Chinese fingerprint examiners document their comparisons during the identification process.The results showed that different examiners demonstrated different accuracy of judgments and different mechanisms to reach them.

Comparison of Vacuum Metal Deposition and 1,2-lndandione/Ninhydrin Reagent Method for the Development of Fingerprints on Renminbi

It is extremely difficult to develop fingerprints from the surface of currency.There are studies reporting that the high vacuum metal deposition(VMD)method can be used to detect fingerprints on certain types of currency notes.Both VMD and 1,2-indandione/ninhydrin techniques are employed to visualize latent fingermarks on porous surfaces,such as paper.The current study explores whether the VMD method or 1,2-indandione/ninhydrin reagent method is more effective in the development of fingerprints on remninbi(RMB).Uncirculated,circulated,and water-exposed RMB was utilized in this study,along with five donors who ranged in their age and potential to leave fingermarks.Samples were aged for a determined period(for uncirculated and circulated RMB,times were 1,3,5,10,and 35 days;for watei^exposed RMB,exposure time was 1 day)and then treated with VMD and 1,2-indandione/ninhydrin.The results suggested that the 1,2-indandione/ninhydrin reagent yielded a better effect for both circulated and uncirculated RMB.For the RMB exposed to water,VMD performed better and gave limited results in terms of fingerprint development,which could serve as a reference for actual forensic cases.

Decreased ^(68)Ga-NOTA-exendin-4 renal uptake in patients pretreated with Gelofusine infusion: a randomized controlled study

Objective:Glucagon-like peptide-1 receptor imaging,using 68Ga-NOTA-exendin-4 is recently established for detecting insulinomas.Owing to the highly radioactive kidneys in 68Ga-NOTA-exendin-4 PET/CT,we aimed to determine the effectiveness of Gelofusine in reducing renal uptake of 68Ga-NOTA-exendin-4.Methods:In our randomized controlled study,patients presenting for 68Ga-NOTA-exendin-4 PET/CT who were18 years and with normal renal function were enrolled,and then randomized into intervention group and control group.Patients in intervention group(group 1)underwent infusion of 500mL Gelofusine prior to PET/CT procedures:patients injected with 68Ga-NOTA-exendin-4(group 1a)immediately after 500mL Gelofusine infusion was completed;(group 1b)at the time point that 250 to 300mL Gelofusine infusion was completed;(group 1c)30minutes after completion of 500mL Gelofusine infusion.The control group(group 2)received 500mL 5%glucose saline instead.PET/CT was then performed,and SUVs of the kidneys and insulinomas were measured.Results:A total of 28 patients were recruited.There were 7 patients(14 kidneys)in each subgroup.The age,body weight,BMI,renal function,injected 68Ga-exendin-4 activity,and interval between injection of the tracer and PET/CT scan were not significantly different among the groups(P>.05).The average and maximum SUVs in the kidneys were significantly reduced in patients of intervention group compared with the control subjects(P<.0001),and the renal uptake could be decreased by 57%with Gelofusine pretreatment.No significant difference in the renal radioactivity was found among group 1a,1b,and 1c(P=.071).The uptake of insulinomas was not significantly different between the intervention and control groups.Conclusion:Renal uptake of 68Ga-exendin-4 can be effectively reduced by pretreatment with Gelofusine.Abbreviations:GLP-1R=Glucagon-like peptide-1 receptor;PRRT=peptide receptor radionuclide therapy;PET=positron emission tomography;SUV=standard uptake value.

Objective evaluation of similarity scores derived by Evofinder^(®) system for marks on bullets fired from Chinese Norinco QSZ-92 pistols

In recent years,many studies have been conducted in the field of firearm identification with the objective of providing an objective method of evaluating the comparison of cartridge cases.However,less attention has been paid to the objective evaluation of bullet comparisons.In this study,1000 registered Chinese Norinco QSZ-92 pistols were used,and a database of 2996 bullets was constructed.Both the receiver operating characteristic(ROC)curve and the score-based likelihood ratio method were used to objectively evaluate the similarity scores derived by the EvofinderVR system.The results indicate that this system has excellent ability to discriminate between the selected pistols.This paper proposes an objective evaluation method,which serves as a response to the ongoing debates regarding the foundation of the discipline.

Specificity of Characteristic Marks on Cartridge Cases from 3070 Consecutive Firings of a Chinese Norinco QSZ‑929 mm Pistol

The specificity of marks was evaluated by differences between marks left by the same firearm(within variability)and differences between marks left by different firearms(between variability).The within variability and between variability of firing pin impressions and breech face marks on cartridge cases from over 3070 consecutive firings of a Chinese Norinco QSZ‑929 mm pistol were investigated based on the automated ballistic identification system Evofinder®.The first 20 cartridge cases and one random specimen out of every set of 10 consecutive cartridge cases of the remaining 3050 cases were entered into the Evofinder®system.Thus,325 cartridges fired from the same pistol were used to estimate the within variability.Then,88 cartridges fired from 88 different pistols of the same model(one cartridge each)were used to estimate the between variability.This study established a database containing 413 cartridges.We used the 325 cartridges fired from the same pistol as a correlation baseline in the database.Both firing pin and breech scores and gaps between these scores were used to analyze the variability.In the score evaluation process,a likelihood ratio system was used to evaluate the likelihood ratio performance.The Evofinder®system correctly discriminated the matches,and the likelihood ratios provided strong support for the true state of the hypothesis.In addition,we found that the score gap was usually greater than the other score differences by more than 1000%in firing pin list and 100%in the breech list.Further analysis of the score and gap can help examiners use the Evofinder®system more efficiently.

Analysis of Microbiome DNA on Frequently Touched Items and from Palm Prints

Limited by the sensitivity of laboratory techniques,conventional human DNA analysis of touch DNA on frequently used items and prints does not always provide satisfactory results.In this study,microbiome DNA on personal computers,cell phones,and palm prints was analyzed and compared.After sample collection,DNA extraction,polymerase chain reaction amplification,library preparation,and sequencing,data were analyzed using the QIIME 1.8.0 software.Weighted unifrac distance between the right palm skin and the right side of a keyboard,the right palm skin and the mouse,and the left side of the keyboard and the left palm skin was 0.258850,0.265474,and 0.214098,respectively.Even after palm prints were left for 1 week,microbial community structures were still quite similar to those of samples collected from the palm skin on the day they were left(weighted unifrac distance was 0.270885).