In order to improve the accuracy of wireless network positioning,the triangulation method of wireless network positioning technology is proposed,which is based on the linear least square fitting method.It makes the ob...In order to improve the accuracy of wireless network positioning,the triangulation method of wireless network positioning technology is proposed,which is based on the linear least square fitting method.It makes the observed value and the fitting value very close,effectively solves the problem of significant contradiction between the fitting result and the observed value in the principle of least square method,and can realize the accurate measurement of geographic information by wireless network positioning technology.展开更多
Based on the analysis of the advantages and disadvantages of GPS positioning system in practical application,this paper proposes the combination of wireless network positioning technology and GPS positioning system to...Based on the analysis of the advantages and disadvantages of GPS positioning system in practical application,this paper proposes the combination of wireless network positioning technology and GPS positioning system to overcome the low accuracy of GPS positioning system in the case of occlusion.This paper introduces in detail the principle of the application of wireless network positioning technology based on GPS positioning system in geographic information measurement,and illustrates its practical application in production by taking coal mine positioning as an example.展开更多
The ent-kaurenoids(e.g.,oridonin and enmein)from the Isodon genus(Lamiaceae)are one class of diterpenoids with rich structural diversity and intriguing pharmaceutical activity.In contrast to the well-established gibbe...The ent-kaurenoids(e.g.,oridonin and enmein)from the Isodon genus(Lamiaceae)are one class of diterpenoids with rich structural diversity and intriguing pharmaceutical activity.In contrast to the well-established gibberellin pathway,oxidative modifications diversifying the ent-kaurene skeleton in Isodon have remained undetermined for half a century.Here we report a chromosome-level genome assembly of I.rubescens,a well-recognized oridonin producer long favored by Asian people as a traditional herb with antitumor effects.The shoot apex was confirmed to be the actual region actively producing ent-kaurene diterpenoids.Through comparative genomics and phylogenetic analyses,we discovered a cluster of tandem-duplicated CYP706V oxygenase-encoding genes located on an ancient genomic block widely distributed in eudicots,whereas almost exclusively emerged in Isodon plants.In the shoot apex,IrCYP706V2 and IrCYP706V7 oxidized the ent-kaurene core in the initial stage of oridonin biosynthesis.Loss of CYP706Vs in other Lamiaceae plants offered an explanation for the specific kaurenoid production in Isodon plants.Moreover,we found that the Isodon genomes encode multiple diterpenoid synthases that are potentially involved in generating diterpenoid diversity.These findings provided new insights into the evolution of the lineage-specific diterpenoid pathway and laid a foundation for improving production of bioactive ent-kaurene-type diterpenoids by molecular breeding and synthetic biology approaches.展开更多
文摘In order to improve the accuracy of wireless network positioning,the triangulation method of wireless network positioning technology is proposed,which is based on the linear least square fitting method.It makes the observed value and the fitting value very close,effectively solves the problem of significant contradiction between the fitting result and the observed value in the principle of least square method,and can realize the accurate measurement of geographic information by wireless network positioning technology.
基金supported by the Key R&D and transformation Projects in Qinghai,China(2019-GX-170).
文摘Based on the analysis of the advantages and disadvantages of GPS positioning system in practical application,this paper proposes the combination of wireless network positioning technology and GPS positioning system to overcome the low accuracy of GPS positioning system in the case of occlusion.This paper introduces in detail the principle of the application of wireless network positioning technology based on GPS positioning system in geographic information measurement,and illustrates its practical application in production by taking coal mine positioning as an example.
基金funded by the National Key R&D Program of China(2018YFA0900600)the National Natural Science Foundation of China(32070328,22077129,and 41876084),the Program of Shanghai Academic Research Leader(20XD1404400)+2 种基金the Strategic Priority Research Program“Molecular Mechanism of Plant Growth and Development”of CAS(XDB27020202)the Construction of the Registry and Database of Bioparts for Synthetic Biology of the Chinese Academy of Sciences(ZSYS-016),the International Partnership Program of the Chinese Academy of Sciences(153D31KYSB20170121),Derivative Bank of Chinese Biological Resources(KFJ-BRP-009-002)the National Key Laboratory of Plant Molecular Genetics,SIPPE,CAS.Y.S.is supported by the Foundation of Youth Innovation Promotion Association of the Chinese Academy of Sciences.
文摘The ent-kaurenoids(e.g.,oridonin and enmein)from the Isodon genus(Lamiaceae)are one class of diterpenoids with rich structural diversity and intriguing pharmaceutical activity.In contrast to the well-established gibberellin pathway,oxidative modifications diversifying the ent-kaurene skeleton in Isodon have remained undetermined for half a century.Here we report a chromosome-level genome assembly of I.rubescens,a well-recognized oridonin producer long favored by Asian people as a traditional herb with antitumor effects.The shoot apex was confirmed to be the actual region actively producing ent-kaurene diterpenoids.Through comparative genomics and phylogenetic analyses,we discovered a cluster of tandem-duplicated CYP706V oxygenase-encoding genes located on an ancient genomic block widely distributed in eudicots,whereas almost exclusively emerged in Isodon plants.In the shoot apex,IrCYP706V2 and IrCYP706V7 oxidized the ent-kaurene core in the initial stage of oridonin biosynthesis.Loss of CYP706Vs in other Lamiaceae plants offered an explanation for the specific kaurenoid production in Isodon plants.Moreover,we found that the Isodon genomes encode multiple diterpenoid synthases that are potentially involved in generating diterpenoid diversity.These findings provided new insights into the evolution of the lineage-specific diterpenoid pathway and laid a foundation for improving production of bioactive ent-kaurene-type diterpenoids by molecular breeding and synthetic biology approaches.