This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content o...This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content of baicalin in S. baicalensis cultivated in Jizhou District was determined using high-performance liquid chromatography( HPLC) to judge whether it met the requirements of Chinese Pharmacopoeia; and according to the requirements of the Chinese Pharmacopoeia of 2015 edition,the contents of heavy metals were determined by ICP-MS. The results showed that the contents of baicalin,Cr,Cu,As,Cd and Pb in S. baicalensis planted in Jizhou District were 14. 625 05%,11. 360,12. 830,0. 062 315,0. 025 715 and 0. 176 65 μg/g,respectively. The content of baicalin in S. baicalensis planted in Jizhou District was much higher than the standard( ≥ 9. 0%) of Chinese Pharmacopoeia of 2015 edition,and the contents of heavy metals were also below the limit( ≤20/1000 000) required by Chinese Pharmacopoeia of 2015 edition.展开更多
[Objectives] This study aimed to evaluate the quality of Salvia miltiorrhiza cultivated in Jizhou. [Methods] The contents of chemical ingredients( tanshinone ⅡA,tanshinone Ⅰ and salvianolic acid B) in S. miltiorrhiz...[Objectives] This study aimed to evaluate the quality of Salvia miltiorrhiza cultivated in Jizhou. [Methods] The contents of chemical ingredients( tanshinone ⅡA,tanshinone Ⅰ and salvianolic acid B) in S. miltiorrhiza were determined by high-performance liquid chromatography( HPLC),and the chemical composition of S. miltiorrhiza planted in Jizhou and purchased from market was compared.[Results]The contents of tanshinone ⅡA,tanshinone I and salvianolic acid B in S. miltiorrhiza planted in Jizhou were 0. 014%,0. 421 2%and 3. 018%,respectively,higher than the standards of the Chinese Pharmacopoeia of the 2015 edition. [Conclusions]The chemical composition of S. miltiorrhiza planted in Jizhou meets the standards of the Chinese Pharmacopoeia of the 2015 edition.展开更多
With seeds as experimental materials,MS was used as the basic medium to combine different species and concentrations of auxin and mitogen for proliferation,elongation and rooting culture. The best combination of mediu...With seeds as experimental materials,MS was used as the basic medium to combine different species and concentrations of auxin and mitogen for proliferation,elongation and rooting culture. The best combination of medium and the most suitable medium were selected. The results showed that the best formula for the medium was MS + 0. 5 mg/L BA + 0. 5 mg/L IAA + 30 g/L sucrose in the proliferation culture,MS + 0. 25 mg/L BA + 0. 5 mg/L IAA + 30 g/L sucrose in the elongation culture,and MS + 0. 5 mg/L IAA + 20 g/L sucrose in the rooting culture respectively. The experimental results will be applied in the rapid propagation and breeding of high-quality seedlings of Platycodon grandiflorus.展开更多
基金Supported by Tianjin Science and Technology Plan Project(16PTZSTG-00020)Talents Project of Tianjin Agricultural University.Tianjin Science and Technology Plan Project(17JCYBJC29800)Various Talent Funding Project of Tianjin Agricultural University(J01009030702)
文摘This study aimed to explore an analysis method for baicalin in Scutellaria baicalensis Georgi and make a reference for the analysis of the medicinal value of S. baicalensis cultivated in Jizhou District. The content of baicalin in S. baicalensis cultivated in Jizhou District was determined using high-performance liquid chromatography( HPLC) to judge whether it met the requirements of Chinese Pharmacopoeia; and according to the requirements of the Chinese Pharmacopoeia of 2015 edition,the contents of heavy metals were determined by ICP-MS. The results showed that the contents of baicalin,Cr,Cu,As,Cd and Pb in S. baicalensis planted in Jizhou District were 14. 625 05%,11. 360,12. 830,0. 062 315,0. 025 715 and 0. 176 65 μg/g,respectively. The content of baicalin in S. baicalensis planted in Jizhou District was much higher than the standard( ≥ 9. 0%) of Chinese Pharmacopoeia of 2015 edition,and the contents of heavy metals were also below the limit( ≤20/1000 000) required by Chinese Pharmacopoeia of 2015 edition.
基金Supported by Tianjin Science and Technology Plan Funding Project(16PTZSTG00020)
文摘[Objectives] This study aimed to evaluate the quality of Salvia miltiorrhiza cultivated in Jizhou. [Methods] The contents of chemical ingredients( tanshinone ⅡA,tanshinone Ⅰ and salvianolic acid B) in S. miltiorrhiza were determined by high-performance liquid chromatography( HPLC),and the chemical composition of S. miltiorrhiza planted in Jizhou and purchased from market was compared.[Results]The contents of tanshinone ⅡA,tanshinone I and salvianolic acid B in S. miltiorrhiza planted in Jizhou were 0. 014%,0. 421 2%and 3. 018%,respectively,higher than the standards of the Chinese Pharmacopoeia of the 2015 edition. [Conclusions]The chemical composition of S. miltiorrhiza planted in Jizhou meets the standards of the Chinese Pharmacopoeia of the 2015 edition.
基金Supported by Science and Technology Planning Project of Tianjin City,China(17JCYBJC29800,16PTZSTG00020)
文摘With seeds as experimental materials,MS was used as the basic medium to combine different species and concentrations of auxin and mitogen for proliferation,elongation and rooting culture. The best combination of medium and the most suitable medium were selected. The results showed that the best formula for the medium was MS + 0. 5 mg/L BA + 0. 5 mg/L IAA + 30 g/L sucrose in the proliferation culture,MS + 0. 25 mg/L BA + 0. 5 mg/L IAA + 30 g/L sucrose in the elongation culture,and MS + 0. 5 mg/L IAA + 20 g/L sucrose in the rooting culture respectively. The experimental results will be applied in the rapid propagation and breeding of high-quality seedlings of Platycodon grandiflorus.
