The pandemic of COVID-19 caused by SARS-CoV-2 has made serious threats to the public health.Antibodies have been considered as promising therapeutics for the prevention and treatment of pathogens.So far,effectors that...The pandemic of COVID-19 caused by SARS-CoV-2 has made serious threats to the public health.Antibodies have been considered as promising therapeutics for the prevention and treatment of pathogens.So far,effectors that can influence the sustainability of SARS-CoV-2 specific antibodies in COVID-19 patients are still unclear.In this paper,we attempted to find potential key factors correlated with SARS-CoV-2 specific antibodies.Transcriptional analysis with the peripheral blood mononuclear cells(PBMCs)revealed proportional changes of immune cell subsets in COVID-19 convalescent patients,including a substantial decrease of monocytes and evident increase of dendritic cells(DCs).Moreover,we found that the gene expressions of chemokines associated with monocyte/macrophage were significantly up-regulated during the COVID-19 recovery phase.Most importantly,we found a set of 27 immune genes corresponding to a comparatively lower amount of SARS-CoV-2 specific antibodies,and identified two hub genes,IL1βand IL6,the protein expressions of which exhibited negative correlation with the immunoglobulin G(IgG)levels in COVID-19 convalescent sera.In addition,we found that high expressions of these 2 hub genes during the convalescent stage were negatively associated with the plasma cell marker CD138.Our study presented two key inflammatory factors correlated to the low level of SARS-CoV-2 specific antibodies,which indicated the potential regulatory process of plasmatic antibodies levels in some COVID-19 convalescent patients.展开更多
基金This study was sponsored by Natural Science Foundation of Chongqing,China(No.cstc2020jcyj-bshX0027),with the donation from Mr Yuling Feng.
文摘The pandemic of COVID-19 caused by SARS-CoV-2 has made serious threats to the public health.Antibodies have been considered as promising therapeutics for the prevention and treatment of pathogens.So far,effectors that can influence the sustainability of SARS-CoV-2 specific antibodies in COVID-19 patients are still unclear.In this paper,we attempted to find potential key factors correlated with SARS-CoV-2 specific antibodies.Transcriptional analysis with the peripheral blood mononuclear cells(PBMCs)revealed proportional changes of immune cell subsets in COVID-19 convalescent patients,including a substantial decrease of monocytes and evident increase of dendritic cells(DCs).Moreover,we found that the gene expressions of chemokines associated with monocyte/macrophage were significantly up-regulated during the COVID-19 recovery phase.Most importantly,we found a set of 27 immune genes corresponding to a comparatively lower amount of SARS-CoV-2 specific antibodies,and identified two hub genes,IL1βand IL6,the protein expressions of which exhibited negative correlation with the immunoglobulin G(IgG)levels in COVID-19 convalescent sera.In addition,we found that high expressions of these 2 hub genes during the convalescent stage were negatively associated with the plasma cell marker CD138.Our study presented two key inflammatory factors correlated to the low level of SARS-CoV-2 specific antibodies,which indicated the potential regulatory process of plasmatic antibodies levels in some COVID-19 convalescent patients.
