Objective:To investigate the effect of matrine against biofilm formation of Escherichia coli(E.coli)in the early stage.Methods:Biofilms of E.coli(ATCC25922)was induced by using a peritoneal dialysis catheter in 96-wel...Objective:To investigate the effect of matrine against biofilm formation of Escherichia coli(E.coli)in the early stage.Methods:Biofilms of E.coli(ATCC25922)was induced by using a peritoneal dialysis catheter in 96-well plates.After treatment with matrine for 24 h,the formation of biofilm on the catheters was observed by scanning electron microscope.The expressions of the flagellar gene(fliC)and motility genes(motA and motB)were determined by qPCR,and the protein expression of fliC was detected by western blotting.Results:The biofilm formation of E.coli was suppressed by different concentrations(less than the minimal inhibitory concentration)of matrine,and the mRNA levels of motA and motB as well as the mRNA and protein expression levels of fliC were decreased in a dose-dependent manner.Conclusion:Matrine could inhibit biofilm formation of E.coli via downregulating the expression of motA,motB and fliC.展开更多
基金supported by the National Natural Science Foundation of China(No. 81360111No.81660133)
文摘Objective:To investigate the effect of matrine against biofilm formation of Escherichia coli(E.coli)in the early stage.Methods:Biofilms of E.coli(ATCC25922)was induced by using a peritoneal dialysis catheter in 96-well plates.After treatment with matrine for 24 h,the formation of biofilm on the catheters was observed by scanning electron microscope.The expressions of the flagellar gene(fliC)and motility genes(motA and motB)were determined by qPCR,and the protein expression of fliC was detected by western blotting.Results:The biofilm formation of E.coli was suppressed by different concentrations(less than the minimal inhibitory concentration)of matrine,and the mRNA levels of motA and motB as well as the mRNA and protein expression levels of fliC were decreased in a dose-dependent manner.Conclusion:Matrine could inhibit biofilm formation of E.coli via downregulating the expression of motA,motB and fliC.
