Zinc(Zn) ferti-fortification using different sources and methods in Zn deficient soils is being advocated to increase Zn concentration in rice kernel as an alternative to pursuing greater Zn-use efficiency(ZnUE). A tw...Zinc(Zn) ferti-fortification using different sources and methods in Zn deficient soils is being advocated to increase Zn concentration in rice kernel as an alternative to pursuing greater Zn-use efficiency(ZnUE). A two-year field study was conducted to assess the effect of Zn application on Zn content and uptake at several growth stages and in several parts of the rice kernel: hull, bran, and the white rice kernel. Variety ‘PB 1509' with 1.25 kg Zn ha^(-1) as Zn-EDTA + 0.5% foliar spray(FS) at maximum tillering(MT) and panicle initiation(PI) stages registered the highest Zn content in hull, bran, and white rice kernel. Among parts of the rice kernel, Zn concentration decreased in the order hull > bran > white rice kernel,indicating that brown rice kernels are much denser in Zn content than polished rice.Considering the higher Zn accumulation in the bran, brown rice consumption, especially in Asia and Africa, could be recommended to overcome Zn malnutrition. The variety ‘PB 1401'showed the highest Zn uptake in rice straw, while ‘PB 1509' showed the highest Zn uptake in hull and white rice kernel. Application of 1.25 kg Zn ha^(-1)(Zn-EDTA) + 0.5% FS at MT and PI and 2.5 kg Zn ha^(-1) ZnSO_4·7H_2O(ZnSHH) + 0.5% FS at MT and PI resulted in higher Zn uptake than other treatments. On average, about one third of total Zn uptake remained in the white rice kernel, with the remaining two thirds accumulating in both hull and bran of brown rice. Zn-EDTA along with 0.5% FS, despite the application of a lower quantity of Zn leading to the highest Zn mobilization efficiency index(ZnMEI) and Zn-induced nitrogen recovery efficiency(ZniNRE), produced the highest kernel yield. However, of the two Zn sources, Zn-EDTA contributed more to the increase in ZnUE than did ZnSHH.展开更多
Rice is very sensitive to low zinc(Zn) supply in submerged paddy soils and Zn deficiency is one of the major limiting factors in determining rice production in India. A field experiment was conducted during the summer...Rice is very sensitive to low zinc(Zn) supply in submerged paddy soils and Zn deficiency is one of the major limiting factors in determining rice production in India. A field experiment was conducted during the summer-rainy seasons of 2009 and 2010 at the research farm of the Indian Agricultural Research Institute, New Delhi, to determine the effects of summer green manure crops and Zn fertilizers on diethylenetriaminepentaacetic acid(DTPA)-extractable(available) Zn concentration in soil and total Zn content in Basmati rice cultivar Pusa Basmati 1 at periodic intervals. Summer green manure crops included Sesbania aculeata(Dhaincha),Crotalaria juncea(Sunhemp), and Vigna unguiculata(Cowpea) and the Zn fertilizers used were ethylenediaminetetraacetic acid(EDTA)-chelated Zn, ZnSO4·7H2O, ZnSO4·H2O, ZnO, and ZnSO4·7H2O + ZnO. Beneficial effects of summer green manure crops and Zn fertilizers on DTPA-extractable Zn concentration in soil and total Zn content in dry matter of Basmati rice at periodic intervals were observed, with significant increases in all the determined parameters, in comparison with those in the control(no Zn application or summer fallow). The rate of increase varied among summer green manure crops and Zn fertilizers during both years. Among the summer green manures, incorporation of S. aculeata led to a significant increase in mean Zn content in Basmati rice grain and straw when compared with C. juncea, V. unguiculata, and summer fallow treatments. Among the Zn fertilizers, significant increases in Zn content in Basmati rice dry matter and DTPA-extractable Zn concentration in soil during various growth stages of the plant were recorded with EDTA-chelated Zn application, followed by the application of ZnSO4·7H2O, ZnSO4·H2O, ZnSO4·7H2O + ZnO, ZnO,and no Zn. The highest mean Zn content in Basmati rice grain and straw was recorded with EDTA-chelated Zn application in 2009 and 2010, respectively. The application of ZnSO4·7H2O was the second best treatment after EDTA-chelated Zn;however, it was statistically inferior to EDTA-chelated Zn. The lowest values were recorded with the control(no Zn application) during both years of study. The amount of Zn concentration in soil was found to be significantly positively correlated with the Zn content in Basmati rice dry matter during both years. Significantly higher levels of residual fertility in soil after the harvest of Basmati rice were observed with application of EDTA-chelated Zn and incorporation of S. aculeata when compared with those of other Zn sources and summer green manures.展开更多
An investigation was undertaken to analyse the influence of microbial inoculants on growth and enzyme activities elicited, and soil microbiome of two varieties of Chrysanthemum morifolium Ramat, which were grown under...An investigation was undertaken to analyse the influence of microbial inoculants on growth and enzyme activities elicited, and soil microbiome of two varieties of Chrysanthemum morifolium Ramat, which were grown under protected mode of cultivation. Rhizosphere soil sampling at 45 and 90 DAT(days after transplanting of cuttings) revealed up to four- to five-fold enhancement in the activity of defence-, and pathogenesisrelated, and antioxidant enzymes, relative to the uninoculated control. Plant growth and soil microbial parameters, especially soil microbial biomass carbon and potential nitrification exhibited significant increases over control. Available soil nitrogen concentrations showed 40%–44% increment in inoculated treatments. Scanning electron microscopy of the root tissues revealed biofilm-like aggregates and individual short bits of cyanobacterial filaments. Analyses of DGGE profiles of archaeal and bacterial communities did not show temporal variations(between 45 and 90 DAT). However,distinct influences on the number and abundance of phylotypes due to microbial inoculants were recorded. The inoculants — Cyanobacterial consortium(BF1- 4) and Anabaena sp.–Trichoderma sp. biofilm(An-Tr) were particularly promising in terms of the plant and soil related parameters,and remained distinct in the DGGE profiles generated. The effect of Trichoderma viride–Azotobacter biofilm on soil bacterial and archaeal communities was unique and distinct as a separate cluster. This study highlights that microbial inoculants exert positive effects, which are specific even to the rhizosphere soil microbiome of chrysanthemum varieties tested. Such inoculants can serve as soil fertility enhancing options in protected floriculture.展开更多
基金the financial assistance received in the form of Senior Research Fellowship from the Director, ICAR-Indian Agricultural Research Institute, New Delhi, India, during his Doctor of Philosophy degree program
文摘Zinc(Zn) ferti-fortification using different sources and methods in Zn deficient soils is being advocated to increase Zn concentration in rice kernel as an alternative to pursuing greater Zn-use efficiency(ZnUE). A two-year field study was conducted to assess the effect of Zn application on Zn content and uptake at several growth stages and in several parts of the rice kernel: hull, bran, and the white rice kernel. Variety ‘PB 1509' with 1.25 kg Zn ha^(-1) as Zn-EDTA + 0.5% foliar spray(FS) at maximum tillering(MT) and panicle initiation(PI) stages registered the highest Zn content in hull, bran, and white rice kernel. Among parts of the rice kernel, Zn concentration decreased in the order hull > bran > white rice kernel,indicating that brown rice kernels are much denser in Zn content than polished rice.Considering the higher Zn accumulation in the bran, brown rice consumption, especially in Asia and Africa, could be recommended to overcome Zn malnutrition. The variety ‘PB 1401'showed the highest Zn uptake in rice straw, while ‘PB 1509' showed the highest Zn uptake in hull and white rice kernel. Application of 1.25 kg Zn ha^(-1)(Zn-EDTA) + 0.5% FS at MT and PI and 2.5 kg Zn ha^(-1) ZnSO_4·7H_2O(ZnSHH) + 0.5% FS at MT and PI resulted in higher Zn uptake than other treatments. On average, about one third of total Zn uptake remained in the white rice kernel, with the remaining two thirds accumulating in both hull and bran of brown rice. Zn-EDTA along with 0.5% FS, despite the application of a lower quantity of Zn leading to the highest Zn mobilization efficiency index(ZnMEI) and Zn-induced nitrogen recovery efficiency(ZniNRE), produced the highest kernel yield. However, of the two Zn sources, Zn-EDTA contributed more to the increase in ZnUE than did ZnSHH.
文摘Rice is very sensitive to low zinc(Zn) supply in submerged paddy soils and Zn deficiency is one of the major limiting factors in determining rice production in India. A field experiment was conducted during the summer-rainy seasons of 2009 and 2010 at the research farm of the Indian Agricultural Research Institute, New Delhi, to determine the effects of summer green manure crops and Zn fertilizers on diethylenetriaminepentaacetic acid(DTPA)-extractable(available) Zn concentration in soil and total Zn content in Basmati rice cultivar Pusa Basmati 1 at periodic intervals. Summer green manure crops included Sesbania aculeata(Dhaincha),Crotalaria juncea(Sunhemp), and Vigna unguiculata(Cowpea) and the Zn fertilizers used were ethylenediaminetetraacetic acid(EDTA)-chelated Zn, ZnSO4·7H2O, ZnSO4·H2O, ZnO, and ZnSO4·7H2O + ZnO. Beneficial effects of summer green manure crops and Zn fertilizers on DTPA-extractable Zn concentration in soil and total Zn content in dry matter of Basmati rice at periodic intervals were observed, with significant increases in all the determined parameters, in comparison with those in the control(no Zn application or summer fallow). The rate of increase varied among summer green manure crops and Zn fertilizers during both years. Among the summer green manures, incorporation of S. aculeata led to a significant increase in mean Zn content in Basmati rice grain and straw when compared with C. juncea, V. unguiculata, and summer fallow treatments. Among the Zn fertilizers, significant increases in Zn content in Basmati rice dry matter and DTPA-extractable Zn concentration in soil during various growth stages of the plant were recorded with EDTA-chelated Zn application, followed by the application of ZnSO4·7H2O, ZnSO4·H2O, ZnSO4·7H2O + ZnO, ZnO,and no Zn. The highest mean Zn content in Basmati rice grain and straw was recorded with EDTA-chelated Zn application in 2009 and 2010, respectively. The application of ZnSO4·7H2O was the second best treatment after EDTA-chelated Zn;however, it was statistically inferior to EDTA-chelated Zn. The lowest values were recorded with the control(no Zn application) during both years of study. The amount of Zn concentration in soil was found to be significantly positively correlated with the Zn content in Basmati rice dry matter during both years. Significantly higher levels of residual fertility in soil after the harvest of Basmati rice were observed with application of EDTA-chelated Zn and incorporation of S. aculeata when compared with those of other Zn sources and summer green manures.
基金funded by the AMAAS Network Project on Microorganisms(IARI Code:12/122)granted by ICAR to RP and the SERB project(SR/S0/PS/164/2010)DST,Government of India granted to BR
文摘An investigation was undertaken to analyse the influence of microbial inoculants on growth and enzyme activities elicited, and soil microbiome of two varieties of Chrysanthemum morifolium Ramat, which were grown under protected mode of cultivation. Rhizosphere soil sampling at 45 and 90 DAT(days after transplanting of cuttings) revealed up to four- to five-fold enhancement in the activity of defence-, and pathogenesisrelated, and antioxidant enzymes, relative to the uninoculated control. Plant growth and soil microbial parameters, especially soil microbial biomass carbon and potential nitrification exhibited significant increases over control. Available soil nitrogen concentrations showed 40%–44% increment in inoculated treatments. Scanning electron microscopy of the root tissues revealed biofilm-like aggregates and individual short bits of cyanobacterial filaments. Analyses of DGGE profiles of archaeal and bacterial communities did not show temporal variations(between 45 and 90 DAT). However,distinct influences on the number and abundance of phylotypes due to microbial inoculants were recorded. The inoculants — Cyanobacterial consortium(BF1- 4) and Anabaena sp.–Trichoderma sp. biofilm(An-Tr) were particularly promising in terms of the plant and soil related parameters,and remained distinct in the DGGE profiles generated. The effect of Trichoderma viride–Azotobacter biofilm on soil bacterial and archaeal communities was unique and distinct as a separate cluster. This study highlights that microbial inoculants exert positive effects, which are specific even to the rhizosphere soil microbiome of chrysanthemum varieties tested. Such inoculants can serve as soil fertility enhancing options in protected floriculture.
