Immobilizing enzymes within metal-organic frameworks(MOFs)enables enzymes to against extreme environments.However,these MOF shells are just like armors,protective but heavy,which shield the enzymes from threats while ...Immobilizing enzymes within metal-organic frameworks(MOFs)enables enzymes to against extreme environments.However,these MOF shells are just like armors,protective but heavy,which shield the enzymes from threats while locking them in the cage.The exploitation of immobilization strategy and intrinsic property of MOFs themselves is of great significance.Here,we proposed a functional protein trap strategy for efficient enzyme encapsulation.The ferrocenedicarboxylic acid(Fc)was used to induce the formation of defect-rich Co-based MOFs(CoBDC-Fc).As result,the engineered protein trap can not only improve the enzyme loading but also accelerate catalytic efficiency.Specifically,the atomically dispersed Fc sites serve as cocatalysts/cofactors and even change the conformation of enzymes in the construed microenvironment.Furthermore,the obtained CoBDC-Fc/enzyme exhibits excellent recyclability and tolerance to inhospitable conditions.Benefited by these,the CoBDC-Fc/enzyme/antigen composites were further prepared for cascade enzyme-linked immunosorbent assay of prostate-specific antigen with satisfactory sensitivity.展开更多
In order to develop pure organic single-molecule white-light emitters(SMWLE),the oxidation of thianthrene(TA)was performed on sulfur atoms at different degrees to tune room temperature phosphorescence(RTP)emission.Wit...In order to develop pure organic single-molecule white-light emitters(SMWLE),the oxidation of thianthrene(TA)was performed on sulfur atoms at different degrees to tune room temperature phosphorescence(RTP)emission.With increasing degrees of oxidation from 1OTA,2OTA,3OTA,to 4OTA,monomeric and aggregative RTP emission was gradually suppressed,due to the gradual disappearance of lone pair electrons on sulfur atoms.Among these compounds,monomers and aggregates of 1OTA demonstrated a better intensity match between fluorescence and RTP.展开更多
A reversible dual-responsive luminescent material was introduced by our group to show the simultaneous color and lifetime switching in response to external stimuli.Pristine crystalline powder of(E)-2-(benzo[d]thiazol-...A reversible dual-responsive luminescent material was introduced by our group to show the simultaneous color and lifetime switching in response to external stimuli.Pristine crystalline powder of(E)-2-(benzo[d]thiazol-2-yl)-3-(pyren-1-yl)acrylonitrile(Py-BZTCN)shows the orderedπ–πstacking with only near-monomer-normal orange-yellow fluorescence,but it exhibits red emission with thermally activated delayed fluorescence(TADF)after grinding,which can be reversibly recovered by heating or fuming treatment.Grinding disturbs the orderedπ–πstacking of pristine powder,leading to the formation of small aggregates with compressed distance and increased overlap ofπ–πstacking between adjacent molecules.The cause of switching was verified by single-crystal X-ray diffraction experiments of two corresponding crystals.This strongπ–πinteraction effectively promotes the excited-state energy splitting and substantially decreases the singlet–triplet energy gap(ΔEST)of aggregates,resulting in the red TADF emission of aggregates through reverse intersystem crossing.This finding proposes a new route to realizing the TADF emission of aggregates through strong intermolecular interactions based on non-TADF monomer,thereby enabling a novel high-contrast dualresponsive luminescence switching.展开更多
基金support of the National Natural Science Foundation of China(Nos.22074049 and 22004042)the Fundamental Research Funds for the Central Universities(No.CCNU22JC006)the Program of Introducing Talents of Discipline to Universities of China(111 program,No.B17019).
文摘Immobilizing enzymes within metal-organic frameworks(MOFs)enables enzymes to against extreme environments.However,these MOF shells are just like armors,protective but heavy,which shield the enzymes from threats while locking them in the cage.The exploitation of immobilization strategy and intrinsic property of MOFs themselves is of great significance.Here,we proposed a functional protein trap strategy for efficient enzyme encapsulation.The ferrocenedicarboxylic acid(Fc)was used to induce the formation of defect-rich Co-based MOFs(CoBDC-Fc).As result,the engineered protein trap can not only improve the enzyme loading but also accelerate catalytic efficiency.Specifically,the atomically dispersed Fc sites serve as cocatalysts/cofactors and even change the conformation of enzymes in the construed microenvironment.Furthermore,the obtained CoBDC-Fc/enzyme exhibits excellent recyclability and tolerance to inhospitable conditions.Benefited by these,the CoBDC-Fc/enzyme/antigen composites were further prepared for cascade enzyme-linked immunosorbent assay of prostate-specific antigen with satisfactory sensitivity.
基金supported by the National Natural Science Foundation of China(62004074,51727809)the Science and Technology Department of Hubei Province(2019AAA063,2020BAA016)。
基金supported by the National Natural Science Foundation of China(51873077,91833304,and 51803071)the National Basic Research Program of China(2016YFB0401001)+1 种基金the Postdoctoral Innovation Talent Support Project(BX20180121)the China Postdoctoral Science Foundation(2018M641767),and JLUSTIRT(2019TD-33).
文摘In order to develop pure organic single-molecule white-light emitters(SMWLE),the oxidation of thianthrene(TA)was performed on sulfur atoms at different degrees to tune room temperature phosphorescence(RTP)emission.With increasing degrees of oxidation from 1OTA,2OTA,3OTA,to 4OTA,monomeric and aggregative RTP emission was gradually suppressed,due to the gradual disappearance of lone pair electrons on sulfur atoms.Among these compounds,monomers and aggregates of 1OTA demonstrated a better intensity match between fluorescence and RTP.
基金supported by the National Natural Science Foundation of China(nos.52073117,91833304,51873077,and 51803071)the National Basic Research Program of China(no.2016YFB0401001)+1 种基金the Postdoctoral Innovation Talent Support Project(no.BX20180121)the China Postdoctoral Science Foundation(nos.2018M641767 and 2019TQ0198).
文摘A reversible dual-responsive luminescent material was introduced by our group to show the simultaneous color and lifetime switching in response to external stimuli.Pristine crystalline powder of(E)-2-(benzo[d]thiazol-2-yl)-3-(pyren-1-yl)acrylonitrile(Py-BZTCN)shows the orderedπ–πstacking with only near-monomer-normal orange-yellow fluorescence,but it exhibits red emission with thermally activated delayed fluorescence(TADF)after grinding,which can be reversibly recovered by heating or fuming treatment.Grinding disturbs the orderedπ–πstacking of pristine powder,leading to the formation of small aggregates with compressed distance and increased overlap ofπ–πstacking between adjacent molecules.The cause of switching was verified by single-crystal X-ray diffraction experiments of two corresponding crystals.This strongπ–πinteraction effectively promotes the excited-state energy splitting and substantially decreases the singlet–triplet energy gap(ΔEST)of aggregates,resulting in the red TADF emission of aggregates through reverse intersystem crossing.This finding proposes a new route to realizing the TADF emission of aggregates through strong intermolecular interactions based on non-TADF monomer,thereby enabling a novel high-contrast dualresponsive luminescence switching.
