Pyrethroid is widely used in developing countries and is a potential threat to human health.So putting forward an effective pyrethroid cleaning method is of vital importance.Clearance of fenpropathrin,cypermethrin and...Pyrethroid is widely used in developing countries and is a potential threat to human health.So putting forward an effective pyrethroid cleaning method is of vital importance.Clearance of fenpropathrin,cypermethrin and deltamethrin in cabbage by using ultrasonic treatment accompanied with different baking soda concentrations were investigated in this study.The results of response surface methodology showed that the maximum clearance rate was 70.61% for fenpropathrin under the optimal power,treated time,treated temperature and baking soda concentration,which were 260.02 W,10 min,20.15◦C and 0.014 g/mL,respectively.Similarly,the maximum removal efficiency was 73.72% for cypermethrin under 240 W,13.84 min,19.53℃ and 0.014 g/mL,and 92.15%for deltamethrin under 238.99 W,12.42 min,25℃ and 0.013 g/mL.The clearance rate of pyrethroid by this method was significant higher(P<0.05)than traditional cleaning methods(bathing and running water washing).The contents of the compositions such as vitamin C,total soluble sugar,protein and dietary fiber,texture and sensory evaluation of cabbage were not found obvious changes after the ultrasonic treatment combined with baking soda(P>0.05).Results showed that ultrasonic treatment combined with baking soda was an effective way to eliminate the fenpropathrin,cypermethrin and deltamethrin in cabbage without influencing cabbage quality.展开更多
Quercetin-3-O-sambubioside[Quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranoside]was separated and purified by semi-preparative high-speed counter-current chromatography(HSCCC)with a twophase-solvent system comp...Quercetin-3-O-sambubioside[Quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranoside]was separated and purified by semi-preparative high-speed counter-current chromatography(HSCCC)with a twophase-solvent system composed of ethyl acetate-nbutanol-water(4∶1∶5,v/v)from the leaves of Nelumbo nucifera(Lotus).A total of 5.0 mg of the targeted compound with a purity of 98.6%as determined by high performance liquid chromatography(HPLC)was obtained from 100 mg of the crude extract cleaned up by AB-8 macroporous resin in a one-step separation.Quercetin-3-O-sambubioside was a novel flavonoid glycoside from the leaves of Nelumbo nucifera,and its chemical structure was identified by means of ESI-MS,1D NMR and 2D NMR.展开更多
Lipophilic(lycopene,β-carotene)and hydrophilic(caffeic acid,p-coumaric acid)phytochemicals were mixed in molar mass at different concentrations/ratios to explore the antioxidant interactions on H_(2)O_(2)-treated H9c...Lipophilic(lycopene,β-carotene)and hydrophilic(caffeic acid,p-coumaric acid)phytochemicals were mixed in molar mass at different concentrations/ratios to explore the antioxidant interactions on H_(2)O_(2)-treated H9c2 cells.Reactive oxide species(ROS),expression of antioxidant enzymes,and oxidation products were analyzed and evaluated by synergistic rate(SR)and combination index(CI).At constant concentrations,antioxidant interactions were influenced by ratios.Group contained more hydrophilic compounds(eg.,p-coumaric acid:β-carotene=9:1)showed antioxidant synergism(SR=22.33±1.23,SR>0)at 3.0μM,while groups that contained more lipophilic compounds(eg.,p-coumaric acid:β-carotene=1:9)showed antioxidant antagonism(SR=-11.68±1.25,SR<0)at 3.0μM.Antioxidant interactions were also influenced by concentrations.From results,ROS generation of group(p-coumaric acid:β-carotene=1:9)changed from synergism(SR=12.27±2.34,SR>0)to antagonism(SR=-11.68±1.25,SR<0)as their total concentrations increased from 1.5μM to 3.0μM.Therefore,this study discussed the possible mechanism of ratio and concentration on the antioxidant interaction among hydrophilic and lipophilic phytochemicals.It also suggested that proper concentrations/ratios of hydrophilic and lipophilic phytochemicals should be considered in diet and formulation of functional foods.展开更多
基金the financial and technology support of State Key Lab of Food Science and Technology of Nanchang University(SKLF-ZZA-201910).
文摘Pyrethroid is widely used in developing countries and is a potential threat to human health.So putting forward an effective pyrethroid cleaning method is of vital importance.Clearance of fenpropathrin,cypermethrin and deltamethrin in cabbage by using ultrasonic treatment accompanied with different baking soda concentrations were investigated in this study.The results of response surface methodology showed that the maximum clearance rate was 70.61% for fenpropathrin under the optimal power,treated time,treated temperature and baking soda concentration,which were 260.02 W,10 min,20.15◦C and 0.014 g/mL,respectively.Similarly,the maximum removal efficiency was 73.72% for cypermethrin under 240 W,13.84 min,19.53℃ and 0.014 g/mL,and 92.15%for deltamethrin under 238.99 W,12.42 min,25℃ and 0.013 g/mL.The clearance rate of pyrethroid by this method was significant higher(P<0.05)than traditional cleaning methods(bathing and running water washing).The contents of the compositions such as vitamin C,total soluble sugar,protein and dietary fiber,texture and sensory evaluation of cabbage were not found obvious changes after the ultrasonic treatment combined with baking soda(P>0.05).Results showed that ultrasonic treatment combined with baking soda was an effective way to eliminate the fenpropathrin,cypermethrin and deltamethrin in cabbage without influencing cabbage quality.
基金funded by the Natural Science Foundation of Jiangxi Province(2006GY0066)the Research Project of Education Office of Jiangxi Province(20030058)the Program for Yangtse Scholars and Innovative Research Team in University(IRT0540).
文摘Quercetin-3-O-sambubioside[Quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-glucopyranoside]was separated and purified by semi-preparative high-speed counter-current chromatography(HSCCC)with a twophase-solvent system composed of ethyl acetate-nbutanol-water(4∶1∶5,v/v)from the leaves of Nelumbo nucifera(Lotus).A total of 5.0 mg of the targeted compound with a purity of 98.6%as determined by high performance liquid chromatography(HPLC)was obtained from 100 mg of the crude extract cleaned up by AB-8 macroporous resin in a one-step separation.Quercetin-3-O-sambubioside was a novel flavonoid glycoside from the leaves of Nelumbo nucifera,and its chemical structure was identified by means of ESI-MS,1D NMR and 2D NMR.
基金funded by the National Natural Science Funds of China(Grant 31972970 and 21964012)found by State Key Laboratory of Food Science and Technology,Nanchang University(Project No.SKLF-ZZB-202119).
文摘Lipophilic(lycopene,β-carotene)and hydrophilic(caffeic acid,p-coumaric acid)phytochemicals were mixed in molar mass at different concentrations/ratios to explore the antioxidant interactions on H_(2)O_(2)-treated H9c2 cells.Reactive oxide species(ROS),expression of antioxidant enzymes,and oxidation products were analyzed and evaluated by synergistic rate(SR)and combination index(CI).At constant concentrations,antioxidant interactions were influenced by ratios.Group contained more hydrophilic compounds(eg.,p-coumaric acid:β-carotene=9:1)showed antioxidant synergism(SR=22.33±1.23,SR>0)at 3.0μM,while groups that contained more lipophilic compounds(eg.,p-coumaric acid:β-carotene=1:9)showed antioxidant antagonism(SR=-11.68±1.25,SR<0)at 3.0μM.Antioxidant interactions were also influenced by concentrations.From results,ROS generation of group(p-coumaric acid:β-carotene=1:9)changed from synergism(SR=12.27±2.34,SR>0)to antagonism(SR=-11.68±1.25,SR<0)as their total concentrations increased from 1.5μM to 3.0μM.Therefore,this study discussed the possible mechanism of ratio and concentration on the antioxidant interaction among hydrophilic and lipophilic phytochemicals.It also suggested that proper concentrations/ratios of hydrophilic and lipophilic phytochemicals should be considered in diet and formulation of functional foods.
