Equipment used in underwater sensing and exploration typically relies on cables or batteries for energy supply,resulting in a limited and inconvenient energy supply and marine environmental pollution that hinder the s...Equipment used in underwater sensing and exploration typically relies on cables or batteries for energy supply,resulting in a limited and inconvenient energy supply and marine environmental pollution that hinder the sustainable development of distributed ocean sensing networks.Here,we design a deep-sea differential-pressure triboelectric nanogenerator(DP-TENG)based on a spiral shaft drive using modified polymer materials to harness the hydrostatic pressure gradient energy at varying ocean depths to power underwater equipment.The spiral shaft structure converts a single compression into multiple rotations of the TENG rotor,achieving efficient conversion of differential pressure energy.The multi-pair electrode design enables the DP-TENG to generate a peak current of 61.7μA,the instantaneous current density can reach 0.69μA cm^(-2),and the output performance can be improved by optimizing the spiral angle of the shaft.The DP-TENG can charge a 33μF capacitor to 17.5 V within five working cycles.It can also power a digital calculator and light up 116 commercial power light-emitting diodes,demonstrating excellent output capability.With its simple structure,low production cost,and small form factor,the DP-TENG can be seamlessly integrated with underwater vehicles.The results hold broad prospects for underwater blue energy harvesting and are expected to contribute to the development of self-powered equipment toward emerging“smart ocean”and blue economy applications.展开更多
Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the me...Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the mechanical properties of cells,characterizing cell physiology,and performing microsurgery.Numerous approaches have been reported for rotating cells in a wide range of academic and industrial applications.Among them,the most popular are micro-robot-based direct contact manipulation and field-based non-contact methods(e.g.,optical,magnetic,electric,acoustic,and hydrodynamic methods).This review first summarizes the fundamental mechanisms,merits,and demerits of these six main groups of approaches,and then discusses their differences and limitations in detail.We aim to bridge the gap between each method and illustrate the development progress,current advances,and prospects in the field of cell rotation.展开更多
The electrical penetration of the cell membrane is vital for determining the cell interior via impedance cytometry.Herein,we propose a method for determining the conductivity of the cell membrane through the tilting l...The electrical penetration of the cell membrane is vital for determining the cell interior via impedance cytometry.Herein,we propose a method for determining the conductivity of the cell membrane through the tilting levels of impedance puises.When electrical penetration occurs,a high-frequency current freely passes through the cell membrane;thus,the intracellular distribution can directly act on the high-frequency impedance pulses.Numerical simulation shows that an uneven intracellular component distribution can affect the tilting levels of impedance pulses,and the tilting levels start increasing when the cell membrane is electrically penetrated.Experimental evidence shows that higher detection frequencies(>7 MHz)lead to a wider distribution of the tilting levels of impedance pulses when measuring cell populations with four-frequency impedance cytometry.This finding allows us to determine that a detection frequency of 7 MHz is able to pass through the membrane of Euglena gracilis(E.gracilis)cells.Additionally,we provide a possible application of four-frequency impedance cytometry in the biomass monitoring of single E.grailis cells.High-frequency impedance(≥7 MHz)can be applied to monitor these biomass changes,and low-frequency impedance(<7 MHz)can be applied to track the corresponding biovolume changes.Overall,this work demonstrates an easy determination method for the electrical penetration of the cell membrane,and the proposed platform is applicable for the multiparameter assessment of the cell state during cultivation.展开更多
基金supported by the National Key R&D Program of China(2021YFC3101300)National Natural Science Foundation of China(42222606,52070006,62103400,42376219,42211540003)+3 种基金Independent Project Deployed by the Innovative Academy of Marine Information Technology of CAS(CXBS202103)2024 Hainan International Science and Technolog.Cooperation Research and Development Project(GHYF2024013)Sanya Science and Technology Special Fund 2022KJCX66CAS Key Laboratory of Science and Technology on Operational Oceanography(No.OOST2021-07).
文摘Equipment used in underwater sensing and exploration typically relies on cables or batteries for energy supply,resulting in a limited and inconvenient energy supply and marine environmental pollution that hinder the sustainable development of distributed ocean sensing networks.Here,we design a deep-sea differential-pressure triboelectric nanogenerator(DP-TENG)based on a spiral shaft drive using modified polymer materials to harness the hydrostatic pressure gradient energy at varying ocean depths to power underwater equipment.The spiral shaft structure converts a single compression into multiple rotations of the TENG rotor,achieving efficient conversion of differential pressure energy.The multi-pair electrode design enables the DP-TENG to generate a peak current of 61.7μA,the instantaneous current density can reach 0.69μA cm^(-2),and the output performance can be improved by optimizing the spiral angle of the shaft.The DP-TENG can charge a 33μF capacitor to 17.5 V within five working cycles.It can also power a digital calculator and light up 116 commercial power light-emitting diodes,demonstrating excellent output capability.With its simple structure,low production cost,and small form factor,the DP-TENG can be seamlessly integrated with underwater vehicles.The results hold broad prospects for underwater blue energy harvesting and are expected to contribute to the development of self-powered equipment toward emerging“smart ocean”and blue economy applications.
基金supported by JSPS Grant-in-Aid for Scientific Research(20K15151)Australian Research Council Discovery Projects(DP200102269)+2 种基金JSPS Core-to-Core programAmada FoundationWhite Rock Foundation。
文摘Cell rotation is one of the most important techniques for cell manipulation in modern bioscience,as it not only permits cell observation from any arbitrary angle,but also simplifies the procedures for analyzing the mechanical properties of cells,characterizing cell physiology,and performing microsurgery.Numerous approaches have been reported for rotating cells in a wide range of academic and industrial applications.Among them,the most popular are micro-robot-based direct contact manipulation and field-based non-contact methods(e.g.,optical,magnetic,electric,acoustic,and hydrodynamic methods).This review first summarizes the fundamental mechanisms,merits,and demerits of these six main groups of approaches,and then discusses their differences and limitations in detail.We aim to bridge the gap between each method and illustrate the development progress,current advances,and prospects in the field of cell rotation.
基金This work is supported by JSPS.Core-to-Core programJSPS Grant-in-Aid for Scientific Research(No.20K15151)+6 种基金Amada Foundation,JapanSasakawa Scientific Research Grant,JapanNSG Foundation,JapanWhite Rock Foundation,JapanAustalian Research Council(ARC)Discovery Project(DP200102269),Australiand the Nara Institute of Science and Technology Support Foundation,JapanJST Support for Pioneering Research Initiated by the Next Generation program and Nara Institute of Science and Technology Touch stone program.
文摘The electrical penetration of the cell membrane is vital for determining the cell interior via impedance cytometry.Herein,we propose a method for determining the conductivity of the cell membrane through the tilting levels of impedance puises.When electrical penetration occurs,a high-frequency current freely passes through the cell membrane;thus,the intracellular distribution can directly act on the high-frequency impedance pulses.Numerical simulation shows that an uneven intracellular component distribution can affect the tilting levels of impedance pulses,and the tilting levels start increasing when the cell membrane is electrically penetrated.Experimental evidence shows that higher detection frequencies(>7 MHz)lead to a wider distribution of the tilting levels of impedance pulses when measuring cell populations with four-frequency impedance cytometry.This finding allows us to determine that a detection frequency of 7 MHz is able to pass through the membrane of Euglena gracilis(E.gracilis)cells.Additionally,we provide a possible application of four-frequency impedance cytometry in the biomass monitoring of single E.grailis cells.High-frequency impedance(≥7 MHz)can be applied to monitor these biomass changes,and low-frequency impedance(<7 MHz)can be applied to track the corresponding biovolume changes.Overall,this work demonstrates an easy determination method for the electrical penetration of the cell membrane,and the proposed platform is applicable for the multiparameter assessment of the cell state during cultivation.
